研究简报

一个新的水稻半矮化小穗突变体的遗传分析与基因定位

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  • 1浙江师范大学 化学与生命科学学院,   浙江 金华 321004;  2 浙江省农业科学院 作物与核技术利用研究所,   浙江 杭州  310021;  3 无锡求是生物农业有限公司;

收稿日期: 2010-12-22

  修回日期: 2011-02-25

  网络出版日期: 2011-11-10

基金资助

浙江省自然科学基金重点项目(Z3110509);国家转基因生物新品种科技重大专项(2008ZX08010002)

Genetic Analysis and Gene Mapping of a New SemiDwarf and Small Panicle Mutant in Rice

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  • 1 College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China; 2 Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences,  Hangzhou 310021, China;  3 Wuxi Qiushi BioAgricultural Co., Ltd.;

Received date: 2010-12-22

  Revised date: 2011-02-25

  Online published: 2011-11-10

摘要

从粳稻中花 11转基因后代中发现了一个半矮化小穗突变体,表现为植株半矮化、生长势弱、半包茎穗、穗型变小等特点,将其命名为sdsp2(semidwarf and small panicle 2)。遗传分析显示,该突变体表型受1对隐性核基因控制。以sdsp2突变体为母本与龙特甫B杂交构建F2分离群体,将该基因定位在水稻第6染色体的RH632和RH640之间的116 kb的物理距离内。通过水稻基因组注释系统在此区域预测到14个开放阅读框,未发现与已报道穗型发育相关基因的同源基因。

本文引用格式

汪庆1,2,3汪得凯2,*陶跃之1,2,* . 一个新的水稻半矮化小穗突变体的遗传分析与基因定位[J]. 中国水稻科学, 2011 , 25(6) : 677 -680 . DOI: 10.3969/j.issn.10017216.2011.06.017

Abstract

A  semidwarf and small panicle mutant was obtained    from  a japonica  rice variety Zhonghua 11 through TDNA transformation. The mutant, characterized by  semidwarf  plant,  abnormal small  and semienclosed  panicle, and poor growth potential   was designated as sdsp2(semidwarf and small panicle 2). Genetic analysis indicated that the mutant was controlled by a single recessive gene. A genetic linkage map was constructed using an  F2 mapping population derived from a cross between  the sdsp2  mutant and an  indica  variety Longtefu B on chromosome 6. The gene was finally located on a 116kb region between the STS markers RH632 and RH640,where 14 ORFs were predicted based on Rice Genome Annotation.

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