利用已公布的OsCRY1a基因的序列设计引物，扩增部分基因片段，构建RNAi植物表达载体并转化水稻，导致该基因表达水平下降和功能缺失。根据转基因植株重要农艺性状的表现，分析该基因的功能。结果表明，抑制OsCRY1a基因的表达，水稻开花期推迟16 d，株高和粒长明显增加，而其他重要农艺性状如剑叶长、宽和穗长等无明显变化。
Using primers designed according to the published sequence of OsCRY1a gene， part of the gene fragment was obtained by PCR and a RNAi expression vector was constructed. The vector was then introduced into rice, leading to the downregulation of the expression level and lossoffunction of the gene. Based on the performance of the transgenic plants, the functions of OsCRY1a was analyzed and deduced. The results indicated that suppressing the expression of OsCRY1a retarded flowering for 16 d with the plant height and grain length significantly increased while other important agronomic traits remained unchanged apparently.
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