水稻microRNA 单核苷酸多态性

于颢, 闫旭, 郭卫东, 辛德东*

doi:10.3969/j.issn.10017216.2011.05.003

中国水稻科学 >

2011 , Vol. 25 >Issue 5: 467 - 474

DOI: https://doi.org/10.3969/j.issn.10017216.2011.05.003

研究报告

水稻microRNA 单核苷酸多态性

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浙江师范大学化学与生命科学学院，浙江金华321004

收稿日期: 2010-12-06

修回日期: 2011-01-08

网络出版日期: 2011-09-10

基金资助

浙江师范大学博士科研启动基金（ZC304009154）

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Single Nucleotide Polymorphism of MicroRNA in Rice

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Chemistry and Life Science College, Zhejiang Normal University, Jinhua 321004, China

Received date: 2010-12-06

Revised date: 2011-01-08

Online published: 2011-09-10

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摘要

microRNA（miRNA）是一类内源性非编码小RNA，长度约22个核苷酸。miRNA通过与靶基因mRNA特定位点结合，调控植物生长、发育和胁迫耐受性。premiRNA的单核苷酸突变会影响miRNA的成熟过程和调控功能，因此，研究miRNA基因中的单核苷酸多态性（SNP）分布对研究miRNA功能分化和基因进化有重要意义。为探讨水稻miRNA基因单核苷酸多态性及其潜在生物学意义，基于现有水稻SNP数据，分析了水稻premiRNA区、侧翼区（位于premiRNA上下游，与premiRNA等长的区间）的SNP分布。与已经报道过的绝大多数生物不同， SNP在水稻premiRNA区的频率与侧翼区无显著差异。进一步研究SNP在premiRNA二级结构中的分布，发现种子区SNP频率跟茎区、环区也无显著差异。然而，SNP在水稻基因区分布跟其他生物相似，即基因区SNP频率显著低于基因侧翼区。这可能暗示premiRNA侧翼区功能对miRNA非常重要，也可能与SNP在不同miRNA基因之间的分布不均衡有关，一部分miRNA基因正经历纯化选择压力放松，积累较多突变而成为新的基因或失去功能。但是以上结果并不排除是由于当前水稻SNP数据可靠性不高而导致的偏差。

关键词： microRNA; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;水稻; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;单核苷酸多态性

本文引用格式

于颢, 闫旭, 郭卫东, 辛德东* . 水稻microRNA 单核苷酸多态性[J]. 中国水稻科学, 2011 , 25(5) : 467 -474 . DOI: 10.3969/j.issn.10017216.2011.05.003

Abstract

MicroRNAs （miRNAs） are a class of endogenous small (about 22 nucleotides), noncoding RNAs, which play important roles in plant growth, development and stress tolerance by binding to the target sites in mRNAs. Since a single nucleotide change in miRNAs can affect the maturity and regulation of miRNA, study on the distribution of single nucleotide polymorphisms (SNPs) in miRNA gene may be of great importance for functional and evolutionary research. In order to explore the potential functional significance of SNPs in the premiRNA in rice, we analyzed the distribution of SNPs in premiRNA region and flanking region, including 5′ and 3′ flanking regions in rice. The results showed that there was no significant difference in the frequency of SNPs between the premiRNA region and its flanking region in rice, which was different from the results in other species. Further study on the frequency of SNPs across premiRNA secondary structures showed that there was no significant difference between seed region and loop region, which was thought to undergo less negative selection pressure in other species. However, in consistent with other species, the frequency of SNPs in proteincoding gene region of rice was significantly lower than that in gene flanking region, indicating that the flanking region of premiRNA may be essential for the function of miRNA. Another reseaon was the uneven distribution of SNPs in different miRNAs. Some miRNAs were less important to rice function, thus was subjected to relaxation of selective pressure, accumulating more and more mutations to become a new gene or loss of functions. However, we could not rule out the bias in the results because of the unreliable data in current SNP database.

Key words： microRNA; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;rice; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;single nucleotide polymorphism

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