一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位

金怡1,2,刘合芹1,汪得凯1,*,陶跃之1,*

doi:10.3969/j.issn.10017216.2011.05.002

中国水稻科学 >

2011 , Vol. 25 >Issue 5: 461 - 466

DOI: https://doi.org/10.3969/j.issn.10017216.2011.05.002

研究报告

一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位

展开

¹ 浙江省农业科学院作物与核技术利用研究所，浙江杭州 310021;² 浙江师范大学化学与生命科学学院，浙江金华 321004;

收稿日期: 2010-12-30

修回日期: 2011-01-19

网络出版日期: 2011-09-10

基金资助

转基因生物新品种培育科技重大专项（2008ZX08010002）；浙江省自然科学基金重点项目（Z3110509）。

收起

Genetic Analysis and Gene Mapping of a White Striped Leaf and White Panicle Mutant in Rice

Expand

¹ Institute of Crop and Nuclear Technology Utilization， Zhejiang Academy of Agricultural Sciences, Hangzhou 310021， China; ²College of Chemistry and Life Science, Zhejiang Normal University, Jinhua 321004, China;

Received date: 2010-12-30

Revised date: 2011-01-19

Online published: 2011-09-10

Fold

摘要

从粳稻中花11组培后代中发现了一个苗期白条纹，抽穗期白穗的突变体。该突变体表现为1叶期叶全白，2叶期从新叶叶尖开始沿叶脉逐渐转绿，至成株期完全变绿，抽穗后内外颖表现为白色，穗轴和小枝梗表现为绿色，成熟后颖壳转黄。根据基因定位结果，将该突变体定名为wslwp(white striped leaf and white panicle)。与野生型相比，wslwp突变体2叶期及抽穗期叶片的叶绿素含量、类胡萝卜素含量及结实率均显著降低。遗传分析表明，该突变表型受1对隐性核基因控制，非TDNA插入引起。为了克隆WSLWP基因，利用wslwp突变体与籼稻品种龙特甫B杂交获得的F2分离群体进行基因定位，首先将该基因定位于水稻第7染色体上的SSR标记RM5711与RM6574之间。随后，利用已有的SSR标记和开发的STS标记，进一步将该基因定位在STS763和STS765之间，物理距离约为87 kb。

关键词： 水稻; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">; 白条纹叶及白穗突变体; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">; 微卫星标记; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;基因定位

本文引用格式

金怡1,2,刘合芹1,汪得凯1,*,陶跃之1,* . 一个水稻苗期白条纹叶及抽穗期白穗突变体的鉴定和基因定位[J]. 中国水稻科学, 2011 , 25(5) : 461 -466 . DOI: 10.3969/j.issn.10017216.2011.05.002

Abstract

A white striped leaf and white panicle mutant, designated as wslwp, was obtained from a rice (Oryza sativa L. ssp. japonica cv. Zhonghua 11) transfer DNA insertion mutant pool. The mutant was characterized by white leaves at the 1leaf stage, chlorotic leaves with longitudinal green stripes at the 2leaf stage, and green leaves before heading. Moreover, at the heading stage, the glume showed white color, but the panicle axis and rachis remained green. The grain changed into yellow at the maturity stage. Chlorophyll and carotenoid contents and seed setting rate of the mutant were significantly reduced. Genetic analysis showed that the phenotype of the mutnt was controlled by a single recessive nuclear gene. An F2 population generated by crossing the mutant with Longtefu B was used as a gene mapping population. WSLWP locus was preliminarily located between SSR molecular markers RM5711 and RM6574 on chromosome 7, then it was mapped to a physical distance 87 kb region between newlydeveloped polymorphic STS markers STS763 and STS765.

Key words： rice; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;white striped leaf ; and white panicle ; mutant; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;simple sequence repeat ; marker; font-family: 宋体; mso-bidi-font-family: 宋体; mso-ansi-language: EN-US; mso-fareast-language: ZH-CN; mso-bidi-language: AR-SA">;gene mapping

参考文献

\[1\]黄晓群, 赵海新, 董春林, 等. 水稻叶绿素合成缺陷突变体及其生物学研究进展. 西北植物学报, 2005, 25(8): 16851691.

\[2\]何冰，刘玲珑，张文伟, 等. 植物叶色突变体. 植物生理学通讯, 2006, 42(1): 19.

\[3\]李娜, 储黄伟, 文铁桥, 等. 水稻白色中脉Oswm 突变体的遗传分析与基因定位. 上海农业学报, 2007, 23(1): 14.

\[4\]Kensuke K, Akiko M, Mitsuo N, et al. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 1997, 12(6): 12411250.

\[5\]Sugimoto H, Kusumi K, Tozawa Y, et al. The virescent2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant Cell Physiol, 2004, 45(8): 985996.

\[6\]Sugimoto H, Kusumi K, Noguchi K, et al. The rice nuclear gene, VIRESCENT 2， is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 2007, 52(3): 512527.

\[7\]胡景涛, 张甲, 李园园, 等. 水稻白色中脉Oswm2的遗传分析与分子标记定位. 遗传, 2008， 30(9): 12011206.

\[8\]Yoo S H, Cho S H, Sugimoto H, et al. Rice Virescent3 and Stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 2009, 150(1): 388401.

\[9\]许凤华, 程治军, 王久林, 等. 水稻白条纹叶Gws基因的精细定位与遗传分析. 作物学报, 2010, 36(5): 713720.

\[10\]Sanchez A C, Khush G S. Chromosomal location of some marker genes in rice using primary trisomics. J Hered, 1994, 85: 297300.

\[11\]李红昌, 钱前, 王斌, 等. 水稻白穗突变体基因的鉴定和染色体定位. 科学通报, 2003, 48(3): 268270.

\[12\]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. Plant Physiol, 1994, 144(3): 307313.

\[13\]Rogers S O, Bendich A J. Extraction of DNA from plant tissues. Plant Mol Biol Manual, 1988, A6: 110.

\[14\]Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to diseaseresistance genes by bulked segregation analysis: A rapid method to detect markers in specific genomic regions by using segregation population. Proc Natl Acad Sci USA, 1991, 88(21): 98289832.

\[15\]Panaud O, Chen X, McCouch S R. Development microsatellite markers characterization of simple sequence length polymorphism (SSLPs) in rice (Oryza sativa L). Mol Gen Genet, 1996, 252(5): 597607.

\[16\]Lander E S, Green P, Abrahamson J, et al. MapMaker: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: 174181.

\[17\]赵海军，吴殿星，舒庆尧，等．携带白化转绿型叶色标记光温敏核不育系玉兔Ｓ的选育及其特征特性．中国水稻科学, 2004, 18(6): 515521.

\[18\]陈涛, 张亚东，赵凌, 等. 一个新的水稻白化转绿突变体的生理特性和基因定位. 遗传学报, 2007, 34(4): 331338.

\[19\]郭士伟, 王永飞, 马三梅, 等. 一个水稻叶片白化转绿叶突变体的遗传分析和精细定位. 中国水稻科学, 2011, 25(1): 9598.

\[20\]Yue R Q, Wang X F, Chen J Y, et al. A rice stromal processing peptidase regulates chloroplast and root development. Plant Cell Physiol, 2010, 51(3): 475485.

\[21\]沈圣泉，舒庆尧, 包劲松, 等．实用转绿型叶色标记不育系白丰Ａ的应用研究．中国水稻科学， 2004， 18(1): 3438．

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献