鉴定了稻瘟病菌孢子萌发过程中分泌产生的蛋白质。将稻瘟病菌菌株200550、200581未萌发孢子、萌发孢子以及含有孢子萌发液的萌发孢子分别接种后，病情指数分别为11.13, 35.06, 49.01和49.6, 8.4, 62.7；说明孢子萌发液中具有能够帮助稻瘟病菌侵染的物质。利用SDSPAGE电泳分析稻瘟病菌孢子萌发液，发现孢子萌发液中含有蛋白质类物质。进一步利用生物质谱鉴定和数据库资源检索，共检索到8个稻瘟病菌的蛋白质基因，其基因编号分别为MGG_01305，MGG_13188，MGG_12454等。提示这些蛋白质可能在稻瘟病菌侵染过程中具有重要的功能。
Secreted proteins during spore germination of Magnaporthe oryzaewere identified. Ungerminated spores, germinated spores and the mixture of germinated spores with germination liquid of M. oryzae (200550, 200581) were inoculated on rice seedlings, respectively. The disease indices were 1113, 3506, 4901 and 4901, 84, 627, indicating that germination liquid played a driving role in infection process. SDSPAGE amplification revealed that secreted proteins were contained. According to the results of mass spectrometer, and database query, eight genes were involved, including MGG_01305, MGG_13188, MGG_12454, etc. The results indicated that these proteins played key roles and important function during M. oryzae infection.
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