在福建省政和县稻田发现大面积的严重水稻根结线虫病，沟渠内禾本科杂草油芒(Eccoilopus sp.)也遭受根结线虫（Meloidogyne sp.）的严重侵染。这两种寄主的根结线虫形态特征与拟禾本科根结线虫(Meloidogyne graminicola，Golden and Birchfield，1965)原始描述相符；对根结线虫的rDNAITS区、28S rDNAD2/D3区进行了扩增、克隆与测序，其序列与GenBank中拟禾本科根结线虫的相似性达99%以上，据此将水稻和油芒上的根结线虫鉴定为拟禾本科根结线虫。通过交叉接种试验和田间调查，证实油芒是水稻根结线虫的重要寄主，为田间水稻根结线虫病的重要侵染源。
The severe occurrence of rice rootknot disease on a large scale was found in Zhenghe, Fujian Province， China. Severe rootknot nematode infestation was also found on grass Eccoilopus sp. of Gramineae in drainage ditch adjacent to rice fields. Morphological characteristics and measurements of root knot nematode on rice or on Eccoilopus sp. were consistent with the original description of Meloidogyne graminicola. The regions of rDNAITS and 28S rDNAD2/D3 of the root knot nematodes were amplified, cloned and sequenced. The sequence each of region had a high degree of genetic similarity (>99%) to the relevant regional sequences of M. graminicola in GenBank. The results showed that the species of rootknot nematodes on rice or Eccoilopus sp. was M. graminicola.The results of habitat investigation and inoculation experiments showed that Eccoilopus sp. was an important host of M. graminicola， and served as an important source of infection of rice rootknot nematode disease in fields.
[1]谢志成.水稻根部线虫鉴定及潜根线虫根结线虫对水稻的致病性[学位论文]. 福州：福建农林大学， 2007： 5-10.
[2]Mulk M M. Meloidogyne graminicola.CIH Descriptions of Plant-parasitic Nematode, Set 6， No. 87. Commonwealth Agricultural Bureau, UK, 1976.
[3]Bridge J, Luc M, Plowight R A. Nematode parasites of rice//Plant Parasitic Nematodes in Subtropical and Tropical Agriculture. Aberystwyty: Cambrian Printers Ltd, 1990: 69-108.
[4]Ejsenback J D， Hirschmann H. Root knot nematodes：Meloidogyne species and races//Manual of Agricultural Nematology．New York: Marcel Dekker, 1991: 191-274.
[5]Padgham J L, Duxbury J M, Mazid A M, et al. Yield loss caused by Meloidogyne graminicola on lowland rainfed rice in Bangladesh. J Nematol, 2004, 36(1): 42-48.
[6]Sharma-Poudyal D, Pokharel R R, Shrestha S M, et al. Evaluation of common Nepalese rice cultivars against rice root knot nematode. Nepal Agric Res J, 2004, 5: 33-36.
[7]Dangal N K, Shrestha S M, Sharma-Poudyal D, et al. Infestation of rice root-knot nematode in rice nurseries in Chitwan. Nepal J Sci Technol, 2009, 10: 45-49.
[8]冯志新， 关燕如， 黎少梅．水稻根结线虫病的研究．华南农学院学报， 1980, 1（1）： 73-82
[9]杨宝君， 胡凯基， 徐炳文．水稻根结属一新种——林氏根结线虫Meloidogyne lini n.sp. 云南农业大学学报, 1988, 3(1): 11-17.
[10]廖金铃， 冯志新． 根结线虫属一新种——海南根结线虫． 华南农业大学学报， 1995, 16(3)：34-39.
[11]赵洪海, 刘维志, 梁晨, 等. 根结线虫在中国的一新纪录种——拟禾本科根结线虫Meloidogyne graminicola.植物病理学报, 2001, 31(2): 184-188.
[12]胡先奇. 根结线虫的种类及其致病性研究[学位论文]. 广州：华南农业大学, 2003: 48-49.
[13]许文耀. 普通植物病理学实验指导. 北京： 科学出版社, 2006: 122.
[14]张绍升. 植物线虫病害诊断与治理. 福州:福建科学技术出版社， 1999: 71-89.
[15]谢辉. 植物线虫分类学. 2版. 北京：高等教育出版社， 2005: 30.
[16]胡永坚, 王暄, 李红梅. 西班牙根结线虫rDNA特性及其检测方法. 南京农业大学学报, 2007, 30(4): 62-65.
[17]Vrain T C, Wakarchuk D A, Levesque A C, et al. Intraspecific rDNA restriction fragment length polymorphism in the Xiphinema americaum group. Fund Appl Nematol, 1992, 15(6): 563-573.
[18]Amiri S, Subbotin S A, Moens M. Identification of the beet cyst nematode Heterodera schachtii by PCR. Eur J Plant Pathol, 2002, 108: 497-506.
[19]Golden A M, Birchfield W. Meloidogyne graminicola (Heteroderidae) a new species of root-knot nematode from grass. Proceedings of the Helminthologleal Society of Washington, 1965， 32： 228-231．
