采用SSR标记分析了304份我国20世纪50-90年代生产上广泛应用的常规稻主栽品种的遗传结构及不同时期籼粳组分的变化。结果显示,我国常规稻主栽品种可明显分为籼、粳两类,籼稻的亚遗传结构比粳稻更为复杂,但晚籼和早粳类型的遗传背景略为单一。早、中、晚各季节类型与遗传结构的分型结果符合度较低。利用12个籼粳分化特异的SSR位点分析各品种的籼粳组分,发现20世纪90年代晚籼类型的粳型组分明显增加,而其他类型不同时期间籼粳组分的差异较小。研究结果对了解我国常规稻品种的遗传结构以及籼粳交育种具有一定的意义。
SSR markers were used to analyze the genetic structure and compare indica or japonica component of Chinese major inbred rice varieties from the 1950s to the 1990s. The results showed that the genetic structure of major inbred rice varieties in China was distinctly divided into two populations, indica and japonica rice. The substructure of indica rice was more complex than that of japonica rice. Lateseason indica rice and earlyseason japonica rice had a little simplex genetic background. The seasonal ecotypes were not quite in conformity with the subtypes of genetic structure. A total of 12 SSR loci with specific differentiation between indica and japonica rice were used to calculate the indica or japonica component. The difference of indica or japonica component among the five decades was not significant except lateseason indica rice. The japonica component of lateseason indica rice planted in the 1990s was increased obviously. These results will be helpful to understand the genetic structure of Chinese major inbred rice varieties and indicajaponica hybridization breeding in China.
[1]Zhang D L, Zhang H L, Wang M X, et al. Genetic structure and differentiation of Oryza sativa L. in China revealed by microsatellites. Theor Appl Genet, 2009, 119(6): 1105-1117.
[2]丁颖. 中国栽培稻的起源及其演变. 农业学报, 1957, 8: 243-260.
[3]杨守仁. 籼粳稻杂交育种研究. 作物学报, 1962, 1(2): 97-102.
[4]杨守仁. 籼粳稻杂交育种研究. 遗传学通讯, 1973, 2: 34-38.
[5]朱立宏. 籼粳稻杂交育种研究. 作物学报, 1964, 3(1): 69-84.
[6]曾世雄, 杨秀青, 卢庄文. 栽培稻籼粳亚种间杂种一代优势的研究. 作物学报, 1980, 6(4): 193-202.
[7]林世成, 闵绍楷. 中国水稻品种及其系谱. 上海: 上海科学技术出版社, 1991: 70-138.
[8]魏兴华, 汤圣祥, 江云珠, 等. 中国栽培稻选育品种等位酶多样性及其与形态学性状的相关分析. 中国水稻科学, 2003, 17(2): 123-128.
[9]齐永文, 张冬玲, 张洪亮, 等. 中国水稻选育品种遗传多样性及其近50年变化趋势.科学通报, 2006, 51(6): 693-699.
[10]华蕾, 袁筱萍, 余汉勇, 等. 我国水稻主栽品种SSR多样性的比较分析. 中国水稻科学, 2007, 21(2): 150-154.
[11]魏兴华, 袁筱萍, 余汉勇, 等. 我国常规稻主栽品种的遗传变异分析. 中国水稻科学, 2009, 23(3): 237-244.
[12] Wei X H, Yuan X P, Yu H Y, et al. Temporal changes in SSR allelic diversity of major rice cultivars in China. J Genet Genom, 2009, 36: 363-370.
[13] Zheng K L, Subudhi P K, Domingo J, et al. Rapid DNA isolation for marker assisted selection in rice breeding. Rice Genet Newsl, 1995, 12: 255-258.
[14]Panaud O, Chen X, McCouch S R. Development of a microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (O. sativa L.). Mol Gen Genet, 1996, 252: 597-607.
[15] Liu K, Muse S V. Integrated analysis environment for genetic marker data. Bioinformatics, 2005, 21: 2128-2129.
[16]Excoffier L, Laval G, Schneider S. Arlequin ver. 3.0: An integrated software package for population genetics data analysis. Evol Bioinform Online, 2005, 1: 47-50.
[17]Falush D, Stephens M, Pritchard J K. Inference of population structure using multilocus genotype data: Linked loci and correlated allele frequencies. Genetics, 2003, 164: 1567-1587.