以一套用籼稻品种9311为受体、粳稻品种日本晴为供体构建的128个染色体片段代换系为材料，按随机区组实验设计种植，于成熟后考查代换系粒长、粒宽性状，利用多元回归分析方法，结合Bin图谱，鉴定出6个与粒长相关的QTL、2个与粒宽相关的QTL。其中，qGL3.1被定位在水稻第3染色体的5 792 954 bp区间内；qGL3.2被定位在第3染色体的917 878 bp区间内；qGL8.1被定位在第8染色体的889 543 bp区间内；qGL8.2被定位在第8染色体的208 614 bp区间内；qGL9.1被定位在第9染色体的1 149 685 bp区间内；qGL11.1被定位在第11染色体的3 184 760 bp区间内；qGW1.1被定位在第1染色体的200 070 bp区间内；qGW5.1被定位在第5染色体的704 905 bp区间内。上述QTL的准确定位，为进一步精细定位及克隆相应QTL和开展水稻粒型分子育种奠定了基础。
徐建军1, 2, 赵强3, 汤在祥1, 赵元凤1, 朱磊1, 徐辰武1, 顾铭洪1, 韩斌3
. 利用重测序的染色体片段代换系群体定位水稻粒型QTL[J]. 中国水稻科学, 2011
, 25(4)
: 365
-369
.
DOI: 10.3969/j.issn.1001-7216.2011.04.004
One hundred and twentyeight chromosome segment substitution lines derived from 9311 as recipient and Nipponbare as donor, were used for mapping QTLs for grain shape by combining the sequencingbased Binmap with a multiple linear regression analysis. Six and two QTLs were identified for grian length(GL) and grian width(GW), respectively. qGL3.1 was mapped in a region of 5 792 954 bp on chromosome 3, qGL3.2 in a region of 917 878 bp on chromosome 3; qGL81 in a region of 889 543 bp on chromosome 8; qGL8.2 in a region of 208 614 bp on chromosome 8; qGL9.1 in a region of 1 149 685 bp on chromosome 9; qGL11.1 in a region of 3 184 760 bp on chromosome 11; qGW1.1 in a region of 200 070 bp on chromosome 1; and qGW5.1 in a region of 704 905 bp on chromosome 5. The results provided a foundation for fine mapping QTLs and subsequent gene cloning.
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