<FONT face="Verdana">一个水稻类树稻突变体(leafy head  3)基因的精细定位</FONT>

吴立文,姜 华,胡 江,胡兴明,高振宇,曾大力,朱观林,董国军,钱 前,郭龙彪

doi:10.3969/j.issn.1001-7216.2009.06.04

中国水稻科学 >

2009 , Vol. 23 >Issue 6: 583 - 588

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.06.04

研究报告

一个水稻类树稻突变体(leafy head 3)基因的精细定位

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中国水稻研究所水稻生物学国家重点实验室，浙江杭州 310006； *通讯联系人， E-mail: guolongb@mail.hz.zj.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-11-10

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Genetic Analysis and FineMapping of a Leafy Head 3 Mutant in Rice

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State Key Laboratory of Rice Biology， China National Rice Research Institute， Hangzhou 310006, China; *Corresponding author, E-mail: guolongb@mail.hz.zj.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-11-10

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摘要

从常规粳稻常优94后代中筛选到一份自然突变的抽穗期延迟的类树稻突变体lhd3（leafy head 3）。在短日照条件下，与野生型比较，lhd3突变体在生长后期，上部节间会继续长出叶片（一般为3片）和高位分蘖，类似于树的侧枝生长，抽穗期延迟，但基部分蘖数不受影响。经典遗传分析表明，lhd3与籼稻南京6号的F2群体中，正常植株与类树稻植株的分离比符合3∶1，说明此性状受单隐性基因控制。利用该群体进行图位克隆，将LHD3基因定位在水稻第1染色体短臂的两个新发展的STS标记wpla3和wpla25之间。再利用5个新发展的STS和CAPS标记，最终将该基因精细定位在WX6和CAPS1两个标记之间，物理距离约为60 kb。通过水稻基因组注释系统共预测到10个开放阅读框（ORF）。对该基因的进一步克隆将有助于阐明水稻生育期和叶原基发育调控机理。

关键词： 水稻; 突变体; 抽穗期; 基因; 精细定位

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吴立文,姜华,胡江,胡兴明,高振宇,曾大力,朱观林,董国军,钱前,郭龙彪 . 一个水稻类树稻突变体(leafy head 3)基因的精细定位[J]. 中国水稻科学, 2009 , 23(6) : 583 -588 . DOI: 10.3969/j.issn.1001-7216.2009.06.04

Abstract

A natural rice mutant lhd3（leafy head 3） with delayed heading date was isolated from progenies of a japonica cultivar, Changyou 94. Under shortday conditions, outgrowth of new leaves (generally 3 leaves) and tillering on upper nodes continues at the late stage of vegetative growth in the lhd3 mutant, which is similar to tree branching. Also it holds the delay in heading date. Classical genetic analysis on F2 population of lhd3/Nanjing 6 indicated that the lhd3 phenotype was controlled by a single recessive gene. LHD3 gene was mapped with the F2 population between two newlydeveloped STS markers, wpla3 and wpla25, on the short arm of rice chromosome 1. Fine mapping delimited it to a 60 kb physical distance between a STS marker and a CAPS marker, WX6 and CAPS1, where 10 ORFs were predicted by Rice Genome Annotation (http://rice.plantbiology.msu.edu/index.shtml).Further cloning of LHD3 will contribute to the clarification of regulation mechanism for growth period and leafprimordium development in rice.

Key words： rice; mutant; heading date; gene; fine mapping

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