<FONT face="Verdana">水稻根尖边缘细胞对铁胁迫的缓解效应</FONT>

宋金敏刘 鹏徐根娣,蔡妙珍,章 艺蔡慧姝

doi:10.3969/j.issn.10017216.2009.05.09

中国水稻科学 >

2009 , Vol. 23 >Issue 5: 503 - 508

DOI: https://doi.org/10.3969/j.issn.10017216.2009.05.09

研究报告

水稻根尖边缘细胞对铁胁迫的缓解效应

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¹浙江师范大学植物学实验室, 浙江金华 321004; ²衢州学院科研处, 浙江衢州 324000; 3浙江省镇海中学，浙江宁波 315200; *通讯联系人, E-mail: sky79@zjnu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-09-10

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Border Cells Alleviating Ferrous Toxicity in Rice Root Tips

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¹Key Laboratory of Botany, Zhejiang Normal University, Jinhua 321004, China; ²Quzhou College, Quzhou 324000, China; ³Zhejiang Zhenhai Middle School, Ningbo 315200, China; *Corresponding author, E-mail: sky79@zjnu.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-09-10

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摘要

从20个水稻品种中筛选出较耐亚铁的水稻品种协优9308和敏感品种IR64，采用静置培养（使边缘细胞附于根尖）和摇床培养（移除根尖边缘细胞），测定根相对伸长率、含水量、根尖保护酶（POD、SOD、CAT）活性以及各根段铁含量，研究边缘细胞对水稻根尖铁胁迫的缓解效应。结果表明，铁毒处理抑制根的伸长、降低幼根的含水量，移除边缘细胞的根相对伸长率以及含水量都低于保留边缘细胞的根，且随着铁毒处理时间的增加，根相对伸长率和幼根的含水量总体呈现下降趋势。在200 mg/L铁毒处理下，根尖保护酶（POD、SOD、CAT）活性受到抑制（除静置培养下协优9308的POD活性外），摇床培养对酶活性的抑制程度较大。铁毒处理使根段铁含量显著上升，静置培养下根段铁含量从0~2 mm到8~10 mm依次增大，摇床培养除8~10 mm根段外其余均无显著差异。说明边缘细胞在水稻根尖铁毒防御中起着一定的作用。

关键词： 水稻; 边缘细胞; 铁胁迫; 过氧化物酶; 超氧化物歧化酶; 过氧化氢酶; 根

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宋金敏刘鹏徐根娣,蔡妙珍,章艺蔡慧姝 . 水稻根尖边缘细胞对铁胁迫的缓解效应[J]. 中国水稻科学, 2009 , 23(5) : 503 -508 . DOI: 10.3969/j.issn.10017216.2009.05.09

Abstract

A Fe2+ tolerant rice variety （Xieyou 9308） and a Fe2+ sensitive variety (IR64) screened from 20 rice varieties were used to study the function of border cells in alleviating ferrous toxicity in rice root tips. The relative elongation rates, water content, activities of peroxidase (POD), superoxide dismutase (SOD), catalase (CAT) and content of Fe in rice root were measured at 0 (CK) and 200 μmol/L Fe2+ for 6 h, 12 h and 24 h respectively, under static culture (to maintain border cells adhered to the root tips) and shaking culture (to remove border cells from root tips completely). The results showed that the relative elongation rates and water content of rice root were reduced by ferrous iron, especially under shaking culture. With the increase of Fe2+ treatment time, the relative elongation rates and water content of rice root declined. At the same time, POD, SOD and CAT activities decreased in 200 mg/L Fe2+ treatment except the POD activity of Xieyou 9308 under the static culture. The restraining rates of POD, SOD and CAT activities were higher in rice root under the shaking culture. The content of Fe in rice root increased heavily and had a significant difference between CK and Fe+ stress treatment group. The content of Fe in section of rice root from 0-2 mm to 8-10 mm was increased gradually under the static culture, but had no significant difference in rice root under the shaking culture except the section of 8-10 mm rice root. These results indicate that root border cells play a significant role in resistance to ferrous toxicity of rice.

Key words： rice; border cell; ferrous toxicity; peroxidase; superoxide dismutase; catalase; root

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