水稻第6染色体短臂上株高QTL qPH6-1的精细定位

鲍钱江樊叶杨,於卫东,陈 忱,范方军,杜景红,庄杰云,

doi:10.3969/j.issn.10017216.2009.05.04

中国水稻科学 >

2009 , Vol. 23 >Issue 5: 470 - 474

DOI: https://doi.org/10.3969/j.issn.10017216.2009.05.04

研究报告

水稻第6染色体短臂上株高QTL qPH6-1的精细定位

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¹中国水稻研究所国家水稻改良中心/水稻生物学国家重点实验室，浙江杭州 310006； ²杭州师范大学生命与环境科学学院, 浙江杭州 310036； *通讯联系人， E-mail: jz1803@hzcnc.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-09-10

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Fine Mapping of QTL qPH6-1 for Plant Height on the Short Arm of Rice Chromosome 6

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¹Chinese National Center for Rice Improvement/State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; ²College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; * Corresponding author, E-mail: jz1803@hzcnc.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-09-10

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摘要

应用衍生于水稻剩余杂合体的分离群体，开展株高QTL的检测和精细定位。应用1个在水稻第6染色体短臂约7.3 Mb区间分离、背景基本纯合的F2:3群体，种植于海南、浙江两地，检测到2个控制株高的QTL；然后，针对两地间作用稳定的qPH61，筛选出3个杂合区间缩小且呈阶梯状排列的单株，衍生F2群体，进一步验证了qPH61的作用，并将其界定于距离为96.4 kb的SSR标记RM3414和RM19417之间；最后，应用分离区间进一步缩小且呈阶梯状排列的3个F2群体，将qPH61定位于距离为51.7 kb的STS标记Si2925和SSR标记RM19417之间。基因组位置比较结果显示，该基因与所有已定位或克隆的水稻矮秆、半矮秆基因均非等位。

关键词： 株高; 数量性状基因座; 精细定位; 剩余杂合体; 水稻

本文引用格式

鲍钱江樊叶杨,於卫东,陈忱,范方军,杜景红,庄杰云, . 水稻第6染色体短臂上株高QTL qPH6-1的精细定位
[J]. 中国水稻科学, 2009 , 23(5) : 470 -474 . DOI: 10.3969/j.issn.10017216.2009.05.04

Abstract

Populations derived from residual heterozygous lines (RHLs) were used for the detection and fine mapping of quantitative trait loci (QTLs) for plant height in rice. An F2:3 population which segregates in a 7.3Mb region on the short arm of rice chromosome 6 in an isogenic background were transplanted in two trial sites in Hainan and Zhejiang Provinces, respectively. Two QTLs for plant height were detected, of which qPH61 exhibited a consistent effect across the two sites. Three plants having smaller heterozygous segments which overlap the qPH61 region were selected. Using the three F2 populations produced, qPH61 was validated and delimitated to a 96.4kb region flanked by SSR markers RM3414 and RM19417. Three more F2 populations which segregate in smaller regions covering qPH61 were developed. The qPH61 locus was delimited to a 51.7kb region flanked by STS marker Si2925 and SSR marker RM19417. It was also shown that this gene is nonallelic to all the major genes for dwarfism mapped or cloned in rice.

Key words： plant height; quantitative trait locus; fine mapping; residual heterozygous line; rice

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