<FONT face="Verdana">中国水稻白叶枯病菌小种C8菌株无毒基因的分离及功能鉴别 <BR></FONT>

纪志远,杨 娟,王寅鹏,周 丹,李玉蓉,邹丽芳,陈功友

doi:10.3969/j.issn.10017216.2009.05.03

中国水稻科学 >

2009 , Vol. 23 >Issue 5: 463 - 469

DOI: https://doi.org/10.3969/j.issn.10017216.2009.05.03

研究报告

中国水稻白叶枯病菌小种C8菌株无毒基因的分离及功能鉴别

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¹南京农业大学植物保护学院/农业部病虫监测与治理重点开放实验室，江苏南京 210095； ²上海交通大学农业与生物学院，上海 200240； ^#共同第一作者； *通讯联系人, E-mail: gyouchen@njau.edu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-09-10

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Identification of a Specific Avirulence Gene from a Chinese Race C8 of Xanthomonas oryzae pv. oryzae

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¹College of Plant Protection/Key Laboratory for Monitoring and Management of Plant Diseases and Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China; ² College of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China; ^# These authors contributed equally to this paper; *Corresponding author, E-mail: gyouchen@njau.edu.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-09-10

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摘要

白叶枯病菌与水稻间的互作符合基因对基因模式，不同小种中存在特异的avrBs3/pthA家族基因。对中国水稻白叶枯病菌小种C8菌株基因组DNA进行BamHⅠ酶切和Southern杂交，发现了其特有的约6.0 kb大小、含avrBs3/pthA家族基因的信号条带。经文库筛选、Southern杂交和限制性酶切分析，获得了C8小种avr/pthC8a基因。将该基因导入菲律宾小种6 的代表菌株PXO99A中，发现avr/pthC8a可使PXO99A丧失对水稻品种Asominori（携有成株期抗性基因Xa17）的毒性，暗示avr/pthC8a可能是与Xa17相应的无毒基因。序列分析发现，avr/pthC8a基因大小为3816 bp，编码1272个氨基酸，由102 bp编码的34个氨基酸重复单元在基因内的重复数为20.5个，其3′端的BamHⅠ酶切位点由GGATCC突变为AGATCC，其他特征均与avrBs3/pthA家族的成员相似，即C端存在1个亮氨酸拉链、3个核定位信号和1个酸性转录激活域。avr/pthC8a基因3′端的BamHⅠ酶切位点发生突变在avrBs3/pthA家族基因中鲜有报道。中国C8小种avr/pthC8a基因的发掘，为进一步了解水稻黄单胞菌毒性变异以及avrBs3/pthA家族基因的进化提供了理论依据。

关键词： 水稻; 白叶枯病菌; 无毒基因; 基因功能

本文引用格式

纪志远,杨娟,王寅鹏,周丹,李玉蓉,邹丽芳,陈功友 . 中国水稻白叶枯病菌小种C8菌株无毒基因的分离及功能鉴别
[J]. 中国水稻科学, 2009 , 23(5) : 463 -469 . DOI: 10.3969/j.issn.10017216.2009.05.03

Abstract

The interaction between Xanthomonas oryzae pv. oryzae (Xoo) and rice follows geneforgene model. A 6.0 kb particular fragment was identified from a Chinese race C8 strain of Xoo, through screening its genomic library by Southern blot with a highly conserved EcoRⅠBamHⅠ fragment of the avrBs3/pthA family as probe. This specific DNA contained an avirulence gene, namely avr/pthC8a, which transmitted the Philippine strain PXO99A from compatible into incompatible interactions with rice Asominori carrying R gene Xa17, suggesting that avr/pthC8a is possibly corresponding for Xa17 specificity. Sequence analysis showed that avr/pthC8a, 3819 bp in size, is a avrBs3/pthA family member, and the repeat number of the 102 bp repeat unit is 20.5. The structure of the gene is almost as same as those of the avrBs3/pthA family genes at 5′ and 3′terminals. The putative protein has a lucine zipper, three nucleotide location signals and an acid transcriptional activation domain at Cterminal. The novel is that the BamHⅠ site at 3′terminal of the gene is mutagenesis from GGATCC into AGATCC, which has not been reported so far. Thus, identification of avr/pthC8a of Chinese race C8 provides a fundamental understanding of virulence differentiation among different races of Xoo and coevolution of avrBs3/pthA genes with rice.

Key words： rice; Xathomonas oryzae pv. oryzae; avirulence gene; gene function

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