<FONT face="Verdana">粳稻垩白性状的QTL检测</FONT>

黎毛毛,徐 磊,任军芳,曹桂兰,余丽琴,高熙宗,贺浩华韩龙植,

doi:10.3969/j.issn.1001-7216.2009.04.06

中国水稻科学 >

2009 , Vol. 23 >Issue 4: 371 - 376

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.04.06

研究报告

粳稻垩白性状的QTL检测

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¹中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/农业部作物种质资源与生物技术重点开放实验室，北京 100081； ²江西农业大学农学院，江西南昌 330045； ³江西省农业科学院水稻研究所，江西南昌 330200； ⁴首尔大学校农业生命科学大学农学科，韩国首尔 151921； *通讯联系人， E-mail: lzhan58@yahoo.com.cn； hhhua64@yahoo.com.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-07-10

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Detection of Quantitative Trait Loci for Chalky Traits in japonica Rice

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¹Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/The National Key Facility for Crop Gene Resources and Genetic Improvement/Key Laboratory of Crop Germplasm Resources and Biotechnology, Ministry of Agriculture, Beijing 100081, China; ²College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, China; ³Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China; ⁴School of Plant Science, College of Agriculture and Life Science, Seoul National University, Seoul 151-921, Korea; *Corresponding author, E-mail: lzhan58@yahoo.com.cn； hhhua64@yahoo.com.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-07-10

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摘要

利用大粒粳稻DL115与小粒粳稻XL005杂交获得的F2群体200个单株为作图群体，采用复合区间作图方法，利用SSR标记对稻米垩白性状进行了数量性状基因座(QTL)检测。研究结果表明，稻米垩白粒率、垩白大小和垩白度在F3株系均呈连续分布，表现为由多基因控制的数量性状。检测到与稻米垩白性状相关的QTL 8个，分别位于第3（5个）、第5（2个）和第6（1个）染色体上，包括与垩白粒率有关的QTL 3个，与垩白大小相关的QTL 2个，与垩白度有关的QTL 3个。其中位于第3染色体RM6832-RM411、RM15456-RM6832和RM6266-RM15456区间的qPGWC3、qACE3b和qDEC3b，分别解释垩白粒率、垩白大小和垩白度表型变异的43.89%、18.83%和19.57%，为主效QTL。上述3个主效QTL所在染色体上的位置与前人研究结果均不一致，认为是新的QTL。所检测到的8个QTL中，除qPGWC6的增效等位基因来自无垩白亲本XL005外，其他7个QTL的增效等位基因均来自垩白性状值较大的亲本DL115。垩白粒率和垩白大小基因作用表现为部分显性，垩白度基因作用表现为加性。

关键词： 粳稻; 垩白性状; 微卫星标记; 数量性状基因座

本文引用格式

黎毛毛,徐磊,任军芳,曹桂兰,余丽琴,高熙宗,贺浩华韩龙植, . 粳稻垩白性状的QTL检测[J]. 中国水稻科学, 2009 , 23(4) : 371 -376 . DOI: 10.3969/j.issn.1001-7216.2009.04.06

Abstract

Using an F2 population including 200 individuals derived from a cross between two japonica rice DL115 with large grain and XL005 with small grain, the quantitative trait loci (QTLs) for chalky traits of rice were identified by composite interval mapping using SSR markers. Three chalky traits, i.e. percentage of grains with chalkiness (PGWC), area of chalky endosperm (ACE) and degree of endosperm chalkiness (DEC), exhibited a continuous distribution in F3 lines, indicating that these chalky traits were quantitative traits controlled by multiple genes. Five, two and one QTLs conferring chalky traits were detected on chromosomes 3, 5 and 6, respectively, including three QTLs for PGWC, two QTLs controlling ACE and three QTL conferring DEC. qPGWC3, qACE3b and qDEC3b were located in RM6832-RM411, RM15456-RM6832 and RM6266-RM15456, and explained 43.89%, 18.83% and 19.57% of the observed phenotypic variance for PGWC, ACE, and DEC, respectively. They were major QTLs and new QTLs. Among the detected eight QTLs, qPGWC6 allele was derived from XL005 without chalky grain, the other seven QTL alleles were derived from DL115 with big chalky grain. The gene actions were partially dominant at PGWC and ACE, and additive at DEC.

Key words： japonica rice; chalky trait; microsatellite marker; quantitative trait locus

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