<FONT face="Verdana">水稻中一个与OsHSP90互作的新基因特性分析</FONT>

马炳田,万佳,张 琦,江 斌,徐永菊,李冬梅,徐正君

doi:10.3969/j.issn.1001-7216.2009.04.02

中国水稻科学 >

2009 , Vol. 23 >Issue 4: 342 - 348

DOI: https://doi.org/10.3969/j.issn.1001-7216.2009.04.02

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水稻中一个与OsHSP90互作的新基因特性分析

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¹四川农业大学水稻研究所，四川温江 611130； ²四川农业大学西南作物基因资源与遗传改良教育部重点实验室，四川雅安 625014； ³四川省农业厅植物检疫站，四川成都 610041； ﹟共同第一作者；*通讯联系人， E-mail: mywildrice@yahoo.com.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2009-07-10

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Characteristic Analysis on a Novel Gene Interacted with OsHSP90 from Rice

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¹Rice Research Institute, Sichuan Agricultural University, Wenjiang 611130, China; ²Key Laboratory of Crop Genetic Resources and Improvement, Ministry of Education, Sichuan Agricultural University, Ya’an 625014, China; ³ Plant Quarantine Station of Sichuan Agriculture Department, Chengdu 610041, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: mywildrice@yahoo.com.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2009-07-10

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摘要

OsHSP90是水稻中一类可以在不同逆境条件下被诱导的基因。利用酵母双杂交系统，在水稻低温和干旱cDNA文库中筛选出1个与OsHSP90互作的未知基因，命名为OsHSPBP。序列分析表明，该基因全长1321 bp，编码一个具有244个氨基酸残基的多肽，推测分子量为25.5 kD。在OsHSPBP蛋白的97-132位氨基酸残基间存在tify功能域，185-211位氨基酸残基构成CCT_2基序，与其他植物中的同源蛋白相似性介于30.66%～70.40%。经分析，该基因启动子区域存在6种不同的逆境反应顺式作用元件。RTPCR半定量结果表明， OsHSP90与OsHSPBP在逆境胁迫下，均能被不同程度地诱导并呈现相似的表达趋势，说明两者可能与水稻逆境胁迫的应答和信号传递有关。

关键词： 水稻; 逆境胁迫; 诱导基因; 基因表达; 氨基酸序列; 酵母双杂交系统

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马炳田,万佳,张琦,江斌,徐永菊,李冬梅,徐正君 . 水稻中一个与OsHSP90互作的新基因特性分析[J]. 中国水稻科学, 2009 , 23(4) : 342 -348 . DOI: 10.3969/j.issn.1001-7216.2009.04.02

Abstract

OsHSP90 is an abiotic stressinducible gene in rice. From the chilling and droughtinducible cDNA library of rice, a novel gene, OsHSPBP, which interacted with OsHSP90 was identified by using yeast twohybrid system. The 1321 bp full length cDNA of OsHSPBP was cloned. Its open reading frame (ORF) predicts a 244amino acid(aa) polypeptide with calculated molecular weight of 25.5 kD. A tify motif and a CCT_2 motif were found at the aa 97-132 and aa 185-211, respectively. Sequence similarity analysis indicated that the OsHSPBP had 30.66%-70.40% identities with the tify family proteins from other plants. Among the putative promoter region, six factors matched to ciselements related to the abiotic stress response were identified using the PlantCARE software. Semiquantitative RTPCR showed that the OsHSP90 and OsHSPBP might be detected in the similar degree under different stresstreatments, suggesting that OsHSP90 and OsHSPBP could be involved in the signal transmission and response to the abiotic stress in rice.

Key words： rice; abiotic stress; inducible gene; gene expression; amino acid sequence; yeast twohybrid system

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