<FONT face="Verdana">通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系</FONT>

范宏环,王林友,张礼霞,于 新,王 曦,金庆生,王建军

doi:10.3969/j.issn.1001-7216.2011.03.016

中国水稻科学 >

2011 , Vol. 25 >Issue 3: 331 - 334

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.03.016

研究简报

通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系

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¹浙江师范大学化学与生命科学学院, 浙江金华 321004; ²浙江省农业科学院作物与核技术利用研究所, 浙江杭州 310021； *通讯联系人, E-mail： zwsjsxz@zaas.org

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2011-05-10

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Breeding of Rice Lines with Bacterial Blight Resistance Gene Xa23 by Using MarkerAssisted Selection

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¹College of Chemistry and Life Sciences, Zhejiang Normal University, Hangzhou 321004, China; ²The Institute of Crops and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; * Corresponding author, E-mail: zwsjsxz @zaas.org

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-05-10

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摘要

通过杂交和分子标记辅助选择技术，将亲本材料18113携有的水稻白叶枯病抗病基因Xa23导入亚种间恢复系H705和优质恢复系H706。利用EST标记C189检测目的基因，在18113/H705 F3和18113/H706 F3株系中，分别获得71和52份携有Xa23纯合基因型恢复系。采用水稻白叶枯病Ⅳ型小种代表菌株浙173进行剪叶接种，分别鉴定出61和44份抗病株系，它们的分子标记辅助选择准确率分别为85.92%和84.61%。研究结果表明，C189是检测Xa23基因的有效分子标记之一，不过，其假阳性的几率高于理论交换率的现象还有待于进一步研究。

关键词： 水稻; 白叶枯病; 分子标记辅助选择

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范宏环,王林友,张礼霞,于新,王曦,金庆生,王建军 . 通过分子标记辅助选择技术选育携有水稻白叶枯病抗性基因Xa23的水稻株系[J]. 中国水稻科学, 2011 , 25(3) : 331 -334 . DOI: 10.3969/j.issn.1001-7216.2011.03.016

Abstract

The rice bacterial blight resistance gene Xa23, which is carried by the donor parent 18113, was introduced into the intersubspecific restorer line H705 and good grain quality restorer line H706 by crossing and molecular markerassisted selection(MAS). Seventyone and 52 homozygous lines carrying Xa23 were obtained from the 18113/H705 F3 and 18113/H706 F3 lines, respectively by PCR with EST marker C189. The bacterial blight resistance of these lines was investigated with the pathotype Ⅳ representative strain Zhe 173 by clipping method. Sixtyone and 44 lines showed the bacterial blight resistance, with the MAS accuracies of 85.92% and 84.61%, respectively. The C189 was one of the effective MAS markers for testing Xa23 gene. In addition, further research is needed to elucidate the mechanisms of higher false positive rate than theoretical exchange rate.

Key words： rice; bacterial blight; markerassisted selection; resistance gene; breeding

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