<FONT face="Verdana">水稻皱曲叶突变体rtl1的遗传分析与分子定位</FONT>

方云霞,宋修娟,彭友林,董国军,郭龙彪,曾大力,张光恒,颜红岚,钱 前,

doi:10.3969/j.issn.1001-7216.2011.03.006

中国水稻科学 >

2011 , Vol. 25 >Issue 3: 261 - 266

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.03.006

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水稻皱曲叶突变体rtl1的遗传分析与分子定位

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¹中国水稻研究所水稻生物学国家重点实验室, 浙江杭州 310006; ²杭州师范大学生命与环境科学学院, 浙江杭州 310016; ³扬州大学教育部植物功能基因组学重点实验室, 江苏扬州 225009; *通讯联系人, E-mail: qianqian188@hotmail.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2011-05-10

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Genetic Analysis and Molecular Mapping of a Rice Rumpled and Twisted Leaf Mutant (rtl1)

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¹State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China; ²College of Life and Environment Science, Hangzhou Normal University, Hangzhou 310016, China; ³Key Laboratory of Plant Functional Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; *Corresponding author, E-mail: qianqian188@hotmail.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-05-10

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摘要

水稻扭曲叶突变体rtl1是利用甲基磺酸乙酯（EMS）诱变粳稻品种日本晴获得的。在苗期，该突变体的叶片就表现出皱缩和扭曲状。将该突变体分别与籼稻品种台中本地1号和浙辐802进行配组。遗传分析表明该突变体性状受1对隐性单基因控制。通过混合分离法，找到了位于第4染色体上的紧密连锁SSR标记RM1155，通过新发展的多态性STS标记，最终将该基因定位在STS标记T1591和SSR标记RM1359之间，其遗传距离分别为0.48和0.96 cM。为进一步克隆该基因打下了基础。

关键词： 水稻; 叶型; 遗传分析; 基因定位; 分子标记

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方云霞,宋修娟,彭友林,董国军,郭龙彪,曾大力,张光恒,颜红岚,钱前, . 水稻皱曲叶突变体rtl1的遗传分析与分子定位[J]. 中国水稻科学, 2011 , 25(3) : 261 -266 . DOI: 10.3969/j.issn.1001-7216.2011.03.006

Abstract

A rumpled and twisted leaf 1 (rtl1) mutant was derived from japonica cultivar Nipponbare by ethyl methane sulfonate (EMS) treatment, which was characterized by rumpled and twisted leaf at the seedling stage. The F2 population was constructed by crossing with indica cultivars TN1 and Zhefu 802, respectively. Genetic analysis confirmed that it was controlled by one recessive nuclear gene. The closely linked SSR marker RM1155 was obtained through bulked segregant analysis．Subsequently, new STS markers were developed using published rice genome sequence, and the gene was finally located between the STS marker T1591 and SSR marker RM1359 with the distances of 0.48 and 0.96 cM, respectively. This will contribute to cloning of the target gene in further studies.

Key words： rice; leaf type; genetic analysis; gene mapping; molecular marker

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