The expressions of active methyl cycle and transfer genes in rice leaves of Zhonghan 3, Shanyou 63 and Aizaizhan were investigated by using genechip and mRNA differential display under 10% PEG6000 simulated drought stress. The results showed that the methyl cycle pathway was activated in leaves of Zhonghan 3 and Shanyou 63, but inhibited in leaves of Aizizhan under drought stress. The expression of methyltransferase genes was induced by drought stress in Zhonghan 3 leaves, especially for the transcription of methylation modification gene of Rubisco, which could reduce the degration of Rubisco protein. Drought stress inhibited the gene transcription of DNA methyltransferse and histone methyltransferse in Aizaizhan leaves. The results confirmed that the active methyl cycle and transfer genes were involved in rice drought resistance.
[1]Espartero J, Pintor-Toro J A, Pardo J M. Differential accumulation of S-adenosylmethionine synthetase transcripts in response to salt stress. Plant Mol Biol, 1994, 25(2): 217-227.
[2]Narita Y, Taguchi H, Nakamura T, et al. Characterization of the salt-inducible methionine synthase from barley leaves. Plant Sci, 2004, 167: 1009-1016.
[3]董建国, 李振国. 乙烯生物合成中间体-1-氨基环丙烷-1-羧酸及其丙二酸结合物的测定. 植物生理学通讯, 1983, 19: 46-48.
[4]Wong W S, Li G G, Ning W, et al. Repression of chilling-induced ACC accumulation in transgenic citrus by over-production of antisense 1-aminocyclopropane-1-carboxylate synthase RNA. Plant Sci, 2001, 161: 969-977.
[5]段辉国, 袁澍, 刘文娟, 等. 多胺与植物逆境胁迫的关系. 植物生理学通讯, 2005, 41(4): 531-536.
[6]葛才林, 杨小勇, 刘向农, 等. 重金属对水稻和小麦DNA甲基化水平的影响. 植物生理与分子生物学学报, 2002, 28(5): 363-368.
[7]Pal-Bhadra M, Bhadra U, Jackson D E, et al. Distinct methylation patterns in histone H3 at Lys-4 and Lys-9 correlate with up- & down-regulation of genes by ethanol in hepatocytes. Life Sci, 2007, 81: 979-987.
[8]肖爱国, 刘荣甫, 黄新平, 等. 中旱3号的特征特性及旱直播标准化栽培技术. 安徽农业学报, 2006, 34(4): 647.
[9]李艳, 马均, 王贺正, 等. 水稻品种苗期抗旱性鉴定指标筛选及其综合评价. 西南农业学报, 2005, 18(3): 250-255.
[10] 戴高兴, 彭克勤, 萧浪涛, 等. 聚乙二醇模拟干旱对耐低钾水稻丙二醛、脯氨酸含量和超氧化物歧化酶活性的影响. 中国水稻科学, 2006, 20(5): 557-559.
[11]Heath R L, Packer L. Photoperoxidation in isolated chloroplasts: Ⅰ. Kinetica and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys, 1968, 125: 189-198.
[12]Eckermann C, Eichel J, Schr der J. Plant methionine synthase: New insights into properties and expression. Biol Chem, 2000, 381: 695-703.
[13]Narita Y, Taguchi H, Nakamura T, et al. Characterization of the salt-inducible methionine synthase from barley leaves. Plant Sci, 2004, 167: 1009-1016.
[14]Ge C L, Wan D Z, Wang Z G, et al. A proteomic analysis of rice seedlings responding to 1,2,4-trichlorobenzene stress. J Environ Sci, 2008, 19(3): 1-11.
[15]Gong Z Z, Koiwa H, Cushman M A, et al. Genes that are uniquely stress regulated in salt overly sensitive (sos) mutants. Plant Physiol, 2001, 126(3): 363-375.
[16]Helena S, Strid A. Six genes strongly regulated by mercury in Pisum sativum roots. Plant Physiol Biochem, 2004, 42(2): 135-142.
[17]Mudegett M B, Clarke S. Hormonal and environmental responsiveness of a developmentally regulated protein repair L-isoaspartyl methy-transferase in wheat. Biol Chem, 1994, 269: 25605-25612.
[18]Nuccio M L, Ziemak M J, Henry S A, et al. cDNA cloning of phosphoethanolamine N-Methyltransferase from spinach by complementation in Schizosaccharomyces pombe and characterization of the recombinant enzyme. Biol Chem J, 2000, 275: 14095-14101.
[19]Smith D D, Summers P S, Weretilnyk E A. Phosphocholine synthesis in spinach: Characterization of phosphoethanolamine N-methyltransferase. Physiol Plant, 2000, 108: 286-294.
[20]Kinney A J, Clarkson D T, Loughman B C. The regulation of phosphatidylcholine biosynthesis in rye( Secale cereale) roots: Stimulation of the nucleotide pathway by low temperature. Biochem J, 1987, 242: 755-759.
[21]赵华燕, 沈庆喜, 吕世友, 等. 水稻咖啡酰辅酶A-O-甲基转移酶基因(CCoAOMT)表达特性分析. 科学通报, 2004, 49(14): 1390-1394.
[22]苏玉, 王溪, 朱卫国. DNA甲基转移酶的表达调控及主要生物学功能. 遗传学报, 2009, 31(11): 1087-1093.
[23]Trievel R C, Flynn E M, Houtz R L, et al. Mechanism of multiple lysine methylation by the SET domain enzyme Rubisco LSMT. Nat Struct Biol, 2003, 10: 545-552.
[24]Albuquerque J A, Esquivel M G, Teixeira A R, et al. The catabolism of ribulose bisphosphate carboxylase from higher plants: A hypothesis. Plant Sci, 2001, 161(1): 55-65.
[25]Zheng Q, Simel E J, Klein P E, et al. Expression, purification, and characterization of recombinant ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit N-methyltransferase. Protein Expr Purif, 1998, 14: 104-112.
[26]Gomez-Cadenas A, Tadeo F R, Talon M, et al. Leaf abscission induced by ethylene in water-stressed intact seedlings of Cleopatra mandarin requires previous abscisic acid accumulation in roots. Plant Physiol, 1996, 112: 401-408.
[27]Tudela D, Primo-Millo E. 1-aminocyclopropane-1-carboxylic acid transported from roots to shoots promotes leaf abscission in Cleopatra mandarin seedlings rehydrated after water stress. Plant Physiol, 1992, 100: 131-137.
[28] Weiss C, Bevan M. Ethylene and a wound signal modulate local and systemic transcription of win2 gene in transgenic potato plants. Plant Physiol, 1991, 96: 943-951.
