The pollen viability of a genetically-modified japonica rice, B2, containing bar gene with herbicide Basta resistance was investigated under nine temperatures(5℃, 10℃, 15℃, 20℃, 25℃, 30℃, 35℃, 40℃, and 45℃) by 2,3,5-triphenyl tetrazolium chloride (TTC) method in vitro after 0, 1, 2, 3, 4, 5, 6, 8, 12, and 14 min from anther. The result showed that the pollen viability decreased with the passing time in vitro, like as “S curve”, which could be accurately described by the Logistic equation. In vitro pollen viability first increased and then decreased with the rising temperature. The optimal temperature for pollen viability ranged from 20℃ to 30℃, and 25℃ was the most optimum temperature. High temperature stress on the pollen viability was more obvious than low temperature. Two equations, an exponential one and a Logistic one, could give the satisfied predicts of the pollen viability duration and pollen longevity, helping to estimate the risk of transgenic flow.
[1]戎俊, 宋志平, 苏军, 等. Bt/GpT1转基因稻及其非转基因亲本对照在间隔种植条件下的转基因漂移. 生物多样性, 2006, 14(4): 309-314.
[2]宋小玲, 强胜, 刘琳莉, 等. 药用野生稻(Oryza officinalis Wall)和转bar基因水稻(Oryza sativa L.)花粉杂交的基因漂移. 南京农业大学学报, 2002, 25(3): 5-8.
[3]Messeguer J, Foghel C, Guiderdoni E, et al. Field assessments of gene flow from transgenic to cultivated rice (Oryza sativa L.) using a herbicide resistance gene as tracer marker. Theor Appl Genet, 2001, 103(8): 1151-1159.
[4]Shivrain V K, Burgos N R, Anders M M, et al. Gene flow between ClearfieldTM rice and red rice. Crop Prot, 2007, 26(5): 349-356.
[5]Grover J. Gene flow study: Implication for GM crop release in Australia. Canberra: Bureau of Rural Sciences, 2002.
[6]李训贞, 梁满中, 周广洽, 等. 水稻开花时的环境条件对花粉活力和结实的影响. 作物学报, 2002, 28(3): 417-420.
[7]郑建初, 盛婧, 汤日圣, 等. 南京和安庆地区高温发生规律及高温对水稻结实率的影响. 江苏农业学报, 2007, 23(1): 1-4.
[8]张彬, 芮雯奕, 郑建初, 等. 水稻开花期花粉活力和结实率对高温的响应特征. 作物学报, 2007, 33(7): 1177-1181.
[9]谢晓金, 李秉柏, 申双和, 等. 抽穗期高温胁迫对水稻花粉活力与结实率的影响. 江苏农业学报, 2009, 25(2): 238-241.
[10]李太贵. 水稻开花期的低温对结实率的影响. 作物学报, 1988, 14(1): 66-70.
[11]Mohammed A R, Tarply L. High nighttime temperatures affect rice productivity through altered pollen germination and spikelet fertility. Agric For Meterol, 2009, 149(6/7): 999-1008.
[12]Prasad P V V, Boote K J, Allen J L H, et al. Species, ecotype and cultivar differences in spikelet fertility and harvest index of rice in response to high temperature stress. Field Crop Res, 2006, 95(2/3): 398-411.
[13]Matsui T, Omasa K, Horie T. High temperature at flowering inhibits swelling of pollen grains, a driving force of thecae dehiscence in rice (Oryza sativa L.). Plant Prod Sci, 2000, 3(3): 430-434.
[14]魏兴华, 袁筱萍, 余汉勇, 等. 转基因插入对水稻花粉活力和杂交结实的影响. 应用生态学报, 2005, 16(1): 115-118.
[15]Zhu B, Huang D N, Yang W, et al. Production of herbicide resistant transgenic rice plants from immature embryos using biolistic method. Sci Agric Sin, 1996, 29(6): 15-20.
[16]富昊伟, 李秋平, 李金军. 转bar基因水稻后代除草剂抗性及农艺性状表现. 浙江农业科学, 2001, 34(5): 247-249.
[17]Hua Z H, Zhu X F, Wu M G, et al. Inheritance of exo-genes integrated into the rice genomes. Acta Agron Sin, 2003, 29(1): 44-48.
[18]Koga Y, Akihama T, Fujimaki H. Study on the longevity of pollen grains of rice, Oryza sativa L.: Ⅰ. Morphological change of pollen grains after shedding. Cytologia, 1971, 36(1): 104-110.
[19]Kumar A, Chowdhury R K, Dahiya O S. Pollen viability and stigma receptivity in relation to meteorological parameters in pearl millet. Seed Sci Technol, 1995, 23(2): 147-156.
[20]徐海波, 王光明, 隗溟, 等. 高温胁迫下水稻花粉粒性状与结实率的相关分析. 西南农业大学学报, 2001, 23(3): 205-207.
[21]郭军洋, 陈龙正, 曹清河, 等. 黄瓜现采花粉生活力最佳染色方法的筛选. 广东农业科学, 2004, 6(1): 48-49.
[22]Khatun S, Flowers T J. The estimation of pollen viability in rice. J Exp Bot, 1995, 46(1): 151-154.
[23]谢晓金, 申双和, 李秉柏, 等. 抽穗期高温胁迫对水稻开花结实的影响. 中国农业气象, 2009, 30(2): 252-256.
[24]Nishiyama I. Effects of temperature on the vegetative growth of rice plants//Proceedings of the Symposium on Climate and Rice. Manila: IRRI, 1974: 159-185.
[25]温婵, 罗海伟, 彭富海, 等. 适宜水稻花粉萌发的液体培养基的优化. 中国农学通报, 2010, 26(2): 68-70.
[26]周光洽, 邓国础, 李训贞, 等. 水稻花粉生活力及其贮藏条件的研究. 湖南农业科学, 1984(2): 17-20.
[27]Song Z P, Lu B R, Chen J K. A study of pollen viability and longevity in Oryza rufipogon, O. sativa, and their hybrids. Int Rice Res Newsl, 2001, 26(2): 31-32.
[28]高亮之, 李林.水稻生态气象. 北京: 农业出版社, 1992: 457-458.
[29]陶龙兴, 沈波, 符冠富, 等. 水稻开花期高温胁迫的研究概述. 浙江农业科学, 2009, 21(1): 4-7.
[30]陈良碧, 肖辉海, 李要民. 不同温湿条件下贮藏的3种禾木科植物花粉的一些生理变化. 热带亚热带植物学报, 2003, 11(3): 236-240.
