<P><FONT face="Verdana">一个水稻叶片白化转绿叶突变体的遗传分析和精细定位</FONT></P>

郭士伟, 王永飞, 马三梅, 李霞,高东迎

doi:10.3969/j.issn.1001-7216.2011.01.015

中国水稻科学 >

2011 , Vol. 25 >Issue 1: 95 - 98

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.015

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一个水稻叶片白化转绿叶突变体的遗传分析和精细定位

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¹江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心，江苏南京210014; ²暨南大学生物工程学系，广东广州 510632

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2011-01-10

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Genetic Analysis and Fine Mapping of a GreenRevertible Albino Leaf Mutant in Rice

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¹Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R ＆ D Center, Nanjing 210014, China; ²Department of Bioengineering, Jinan University, Guangzhou 510632, China

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-01-10

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摘要

在水稻品种宜香B中发现了一个白化转绿叶突变体，经过多代自交获得了稳定的白化转绿叶色突变体。该突变体在4叶期前叶色为黄绿色，之后逐渐变绿，从苗龄4周到12周，突变体/野生型叶绿素含量比值从34.5%逐渐升高到99.4%。遗传分析表明该突变受1对隐性核基因控制，暂命名为gra。利用微卫星标记将gra初步定位于第10染色体标记RM596和RM5620之间，进一步利用极端个体定位法把gra精细定位于标记RM25522和RM25535之间。gra基因距RM25522和RM25535标记的遗传距离均为0.05 cM，其物理距离约为136 kb。

关键词： 水稻; 白化转绿叶突变体

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郭士伟, 王永飞, 马三梅, 李霞,高东迎 .

一个水稻叶片白化转绿叶突变体的遗传分析和精细定位

[J]. 中国水稻科学, 2011 , 25(1) : 95 -98 . DOI: 10.3969/j.issn.1001-7216.2011.01.015

Abstract

A greenrevertible albino leaf mutant was found in an indica rice variety, Yixiang B, in the field. A stably inherited mutant with greenrevertible albino leaf was obtained after five successive generations of selfcrossing, and temporarily named as gra. The leaf color of gra was albino before 4th leaf stage, and then gradually turned green. As the content of chlorophyll in leaves increased from 4weekold to 12weekold seedlings, the ratio of mutant/wild chlorophyll content in rice leaves rose from 34.5% to 99.4%. Genetic analysis of F2 populations showed that the mutation was controlled by a single recessive nuclear gene. The gene was finely mapped between the SSR markers RM25522 and RM25535, with genetic distances of 0.05 cM to each marker, respectively, and a physical distance of 136 kb.

Key words： rice; greenrevertible albino leaf mutant; gene; fine mapping

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