利用Wx和fgr基因双功能性标记高效选育优质水稻保持系

陈深广,周屹峰,赵霏,金亮,沈圣泉,

doi:10.3969/j.issn.1001-7216.2011.01.005

中国水稻科学 >

2011 , Vol. 25 >Issue 1: 31 - 36

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.005

研究报告

利用Wx和fgr基因双功能性标记高效选育优质水稻保持系

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¹中国水稻研究所，浙江杭州310006； ²浙江大学农业与生物技术学院，浙江杭州 310029； *通讯联系人， E-mail: shenshq@zju.edu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2011-01-10

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Efficient Selection of Fine Rice Quality Maintainer Lines with Functional Markers of Wx and fgr Genes

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¹China National Rice Research Institute, Hangzhou 310006, China; ²College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China; *Corresponding author, E-mail: <A href=

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-01-10

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摘要

对选取的13份籼型保持系进行直链淀粉含量和香味测定及分子标记（484/485和fgr1F/1R）检测，筛选出宜香1B作为试验用分子育种优质供体亲本。配组Ⅱ32B/宜香1B，F2代进行该双标记分离分析，结果表明，不仅F2单株间特异性谱带差别明显便于鉴别，且呈现出两对独立遗传基因分离模式。选取F2代 484/485和fgr1F/1R呈Ⅰ型（与宜香B相同的带型）和Ⅲ型（杂合带型）的优良单株，经F3~F6加代，并结合农艺性状（株型、播抽历期等）和稻米品质性状（粒型、垩白度、透明度等）鉴定和选择，在F6再利用484/485和fgr1F/1R分子标记进行复测和验证，从而获得了一批株系内性状基本稳定、株型好、直链淀粉含量中等、具有香味、外观和蒸煮食用品质优良的高世代株系。经测交鉴定，发现其保持系株系比例约占75.5%。表明利用Wx和fgr基因功能性分子标记的早世代检测和辅助选择，结合常规优质保持系鉴定和选育方法，可以快速获得直链淀粉含量中等、香味、长粒型、透明、低垩白、较易糊化等综合性状较优的保持系。

关键词： 籼稻; 分子标记辅助选择; 直链淀粉含量; 香味; 功能性标记

本文引用格式

陈深广,周屹峰,赵霏,金亮,沈圣泉, . 利用Wx和fgr基因双功能性标记高效选育优质水稻保持系[J]. 中国水稻科学, 2011 , 25(1) : 31 -36 . DOI: 10.3969/j.issn.1001-7216.2011.01.005

Abstract

According to the genotypes detected by two primers of functional markers, 484/485 (for Wx gene) and fgr1F/1R(for fgr gene), amylose contents and fragrance of 13 indica maintainer lines, Yixiang 1B was selected as good rice quality donor parent. The two markers were used to genotype the F2 individual plants derived from Ⅱ32B/Yixiang 1B. There were significant difference in electrophoretogram among individual plants, so that they were easy to distinguish, and these two markers showed independent inheritance. Superior individuals with fgr and Wx genotypeⅠ(same as Yixiang 1B) and Ⅲ (heterozygote) were selected. Then combined with the selection of agronomic and rice quality characters, the detection in selected plants or individual plants in F3 to F6 generations were carried out. In F6 generation, the plants were detected by 484/485 and fgr1F/1R again, advanced plants were obtained, which had stable characters, good plant type, obvious fragrance, medium amylose content and so on. Results of testcross showed that 75.5% plants were maintainer lines. It is suggested that detection and selection of maintainer lines of indica rice with functional markers of Wx and fgr genes in earlygeneration combined with conventional breeding could provide a rapid way to obtain good rice quality maintainer lines with fragrance, medium amylose content, slender grain shape, low chalkiness degree, high transparency and so on.

Key words： indica rice; markerassisted selection; amylose content; fragrance; functional marker

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