<FONT face="Verdana">抗稻瘟病基因<EM>Pi9</EM>的STS连锁标记开发及在分子标记辅助育种中的应用</FONT>

殷得所,夏明元,李进波,万丙良,査中萍,杜雪树,戚华雄

doi:10.3969/j.issn.1001-7216.2011.01.004

中国水稻科学 >

2011 , Vol. 25 >Issue 1: 25 - 30

DOI: https://doi.org/10.3969/j.issn.1001-7216.2011.01.004

研究报告

抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用

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湖北省农业科学院粮食作物研究所, 湖北武汉 430064; *通讯联系人， E-mail: qihx@hbaas.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2011-01-10

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Development of STS Marker Linked to Rice Blast Resistance Gene Pi9 in Marker Assisted Selection Breeding

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Food Crops Institute, Hubei Academy of Agricultural Sciences, Wuhan 430064, China; *Corresponding author, E-mail: qihx@hbaas.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2011-01-10

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摘要

利用带有广谱抗稻瘟病基因Pi9的籼稻品系751127作为抗病基因供体亲本，用于扬稻6号和R6547抗病性的回交育种。通过比较751127、扬稻6号和日本晴的Pi9基因位点的DNA序列，开发出了与Pi9基因紧密连锁的共显性STS（序列标记位点）标记PB91，用于Pi9基因的分子标记辅助选择。结合田间农艺性状选择和分子标记辅助选择，培育出8个Pi9基因纯合的回交后代株系。其中，具有扬稻6号和R6547遗传背景的株系各4个。经湖北恩施和宜昌的病圃鉴定，携带有抗病基因株系的稻瘟病抗性水平较受体品种扬稻6号和R6547有不同程度的提高。具有R6547遗传背景的株系08C893配制的杂交组合在上述病区的抗性表现也明显优于对照品种扬两优6号。上述结果说明，共显性标记PB91在Pi9抗稻瘟病基因分子标记辅助育种中具有应用价值，并且Pi9基因作为稻瘟病抗源之一可以在湖北稻区进行有效利用。

关键词： 稻瘟病抗性; 分子标记辅助选择; 序列标记位点; 抗性基因; 育种; 水稻

本文引用格式

殷得所,夏明元,李进波,万丙良,査中萍,杜雪树,戚华雄 . 抗稻瘟病基因Pi9的STS连锁标记开发及在分子标记辅助育种中的应用[J]. 中国水稻科学, 2011 , 25(1) : 25 -30 . DOI: 10.3969/j.issn.1001-7216.2011.01.004

Abstract

Indica rice line 751127 carrying broadspectrum rice blast resistance gene Pi9 was used as resistance gene donor for genetic improvement of Yangdao 6 and R6547. By alignment of genomic sequences from the Pi9 locus of 751127, Yangdao 6 and Nipponbare, codominant STS (sequencetagged site) marker PB91, which linked to the Pi9 gene, was developed for molecular markerassisted selection (MAS). Eight backcross lines carrying Pi9 were obtained, among which four lines contained the genetic background of Yangdao 6 and the other four lines had the R6547 background. Under natural disease infection conditions in Enshi County and Yichang County of Hubei Province, China, the Pi9carrying lines showed higher blast disease resistance than Yangdao 6 and R6547 in various degrees. The hybrids originated from 08C893, an R6547derived rice line, showed higher blast disease resistance as compared to the control Yangliangyou 6. The results suggest PB91 is an effective codominant marker for MAS of Pi9 which is a valuable resistance gene for the control of rice blast disease in Hubei area.

Key words： blast resistance; molecular markerassisted selection; sequencetagged site; resistance gene; breading; rice

参考文献

[1]Baker B, Zambryski P, Staskawicz B, et al. Signaling in plant-microbe interactions. Science, 1997, 276(5313): 726-733.

[2]董继新, 董海涛, 李德葆. 水稻抗瘟性研究进展. 农业生物技术学报, 2000, 8(1): 99-120.

[3]杨勤忠, 林菲, 冯淑杰, 等. 水稻稻瘟病抗性基因的分子定位及克隆研究进展. 中国农业科学, 2009, 42(5): 1601-1615.

[4]吴俊, 刘雄伦, 戴良英, 等. 水稻广谱抗稻瘟病基因研究进展. 生命科学, 2007, 19(2): 233-238.

[5]Hittalmani S, Parco A, Mew T W, et al. Fine mapping and DNA marker-assisted pyramiding of the three major genes for blast resistance in rice. Theor Appl Genet, 2000, 100(7):1121-1128.

[6]Narayanan N N, Baisakh N, Oliva N P, et al. Molecular breeding: Marker-assisted selection combined with biolistic transformation for blast and bacterial blight resistance in indica rice (cv. CO39). Mol Breed, 14(1): 61-71.

[7]陈学伟, 李世贵, 黎汉云, 等. 水稻抗稻瘟病基因Pi-d(t)、Pi-b、 Pi-ta2的聚合及分子标记选择. 生物工程学报, 2004, 20(5): 708-714.

[8]陈志伟, 郑燕, 吴为人, 等. 抗稻瘟病基因Pi-2(t)紧密连锁的SSR标记的筛选与应用. 分子植物育种, 2004, 2(3): 321-325.

[9]柳武革, 王丰, 金素娟, 等. 利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系的稻瘟病抗性. 作物学报, 2008, 34(7): 1128-1136.

[10]陈红旗, 陈宗祥, 倪深, 等. 利用分子标记技术聚合3个稻瘟病基因改良金23B的稻瘟病抗性. 中国水稻科学, 2008, 22(1): 23-27.

[11]倪大虎, 易成新, 李莉, 等. 利用分子标记辅助选择聚合水稻基因Xa21和Pi9(t). 分子植物育种, 2005, 3(3): 329-334.

[12]陈健民, 付志英, 王锋, 等. 分子标记辅助培育双抗稻瘟病和白叶枯病杂交稻恢复系. 分子植物育种, 2009, 7(3): 465-470.

[13]Liu G F, Lu G, Zeng L R, et al. Two broad-spectrum blast resistance genes, Pi9(t) and Pi2(t), are physically linked on rice chromosome 6. Mol Genet Genomics, 2002, 267(4): 472-480.

[14]Qu S H, Liu G F, Wang G L, et al. The broad-spectrum blast resistance gene Pi9 encodes a nucleotide-binding site-leucine-rich repeat protein and is a member of a multigene family in rice. Genetics, 2006, 172(3): 1901-1914.

[15]Murray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acid Res, 1980, 8(19): 4321-4325.

[16]李进波, 夏明元, 戚华雄, 等. 水稻抗褐飞虱基因Bph14和Bph15的分子标记辅助选择. 中国农业科学, 2006, 39(10): 2132-2137.

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