<FONT face="Verdana">利用SSR分析普通野生稻自然居群交配系统</FONT>

李小湘, 刘勇,段永红,王淑红,詹庆才,孙桂华,高立志,

doi:10.3969/j.issn.1001-7216.2010.06.007

中国水稻科学 >

2010 , Vol. 24 >Issue 6: 601 - 607

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.06.007

研究报告

利用SSR分析普通野生稻自然居群交配系统

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¹湖南省农业科学院水稻研究所, 湖南长沙 410125； ²中南大学研究生院, 湖南长沙 410083;³中国科学院昆明植物研究所, 云南昆明 650204；*通讯联系人, E-mail: haoasliu@163.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-11-10

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Estimation of Mating System in Natural Oryza rufipogon Populations by SSR Markers

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¹Rice Research Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, China; ²Graduate School of Central South University, Changsha 410083, China; ³Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650204, China; *Corresponding author, E-mail: haoasliu@163.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-11-10

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摘要

采用SSR标记技术检测了6个自然居群的遗传多样性和交配系统参数，旨在了解其居群交配系统，为有效原位保护、异位保存提供指导。结果表明，6个居群遗传多样性水平存在差异（基因多样性指数变幅为0.3166～0.6075），居群间分化明显（Fst=0.4220）。广东高州大岭居群和广东高州朋山居群有过剩的杂合子（F<0）,而另外4个居群有过剩的纯合体（F>0）。普通野生稻6个取样居群交配系统为混合交配类型，居群之间的异交率相差较大（多位点异交率变幅为15.1%～418%，单位点异交率变幅为10.1%～28.5%）。6个居群的多位点异交率都高于单位点异交率，而且位点亲本相关度比较大，说明居群内存在一定程度的近交。同时，各居群单位点相关度与多位点相关度差值较大，表明居群内存在亚结构。还讨论了进一步改善普通野生稻原位和异位保护的措施。

关键词： 普通野生稻; 交配系统; 原位保护; 异位保存; 遗传多样性; 简单重复序列

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李小湘, 刘勇,段永红,王淑红,詹庆才,孙桂华,高立志, . 利用SSR分析普通野生稻自然居群交配系统[J]. 中国水稻科学, 2010 , 24(6) : 601 -607 . DOI: 10.3969/j.issn.1001-7216.2010.06.007

Abstract

Oryza rufipogon Griff．is the most important germplasm for rice improvement．Better understanding its mating system will provide useful information for further in situ conservation and management of O. rufipogon． By using SSR method，the genetic diversity and outcrossing rate of six natural O.rufipogon populations were estimated. The levels of genetic diversity varied among the populations, with the Nei′s diversity indices ranged from 0.3166 to 0.6075. There was a significant genetic differentiation (Fst=0.422) among the sampled populations. Two populations exhibited a heterozygosity excess, whereas the other four populations had a heterozygosity deficit. The O.rufipogon populations possessed a mixed type of mating system, although the outcrossing rate was variable among the populations. The multilocus outcrossing rate ranged from 15.1% to 418%，and the singlelocus outcrossing rate from 10.1% to 28.5%．There were significant differences between the multilocus outcrossing rate and the singlelocus outcrossing rate, and high values of correlation of paternity in the populations, indicating a considerable inbreeding existed in the populations. The correlation of paternity for the multilocus significantly differed from the correlation of paternity for the singlelocus, suggesting that there was a substructure occurred in each sampled population. Some measures for further improving insitu and exsitu conservation of O. rufipogon were also discussed.

Key words： Oryza rufipogon; mating system; in-situ conservation; ex-situ conservation; genetic diversity; simple sequence repeats

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