<FONT face="Verdana">利用分子标记辅助选择改良水稻恢复系的稻瘿蚊抗性</FONT>

柳武革,王丰,肖汉祥,刘振荣,李金华,廖亦龙, 朱满山,黄慧君,黄炳超,张扬

doi:10.3969/j.issn.1001-7216.2010.06.004

中国水稻科学 >

2010 , Vol. 24 >Issue 6: 581 - 586

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.06.004

研究报告

利用分子标记辅助选择改良水稻恢复系的稻瘿蚊抗性

展开

¹广东省农业科学院水稻研究所，广东广州 510640； ²广东省农业科学院植物保护研究所，广东广州 510640； *通讯联系人， E-mail:fwang1631@163.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-11-10

收起

Improvement of Gall Midge Resistance for Restorer Line in Rice by Molecular
Marker Assisted Selection

Expand

¹Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China； ²Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China； *Corresponding author, E-mail: fwang1631@163.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-11-10

Fold

摘要

以含有华南4种稻瘿蚊生物型显性抗性基因Gm6的籼稻材料KG18为供体，三系优质恢复系广恢998为受体，通过杂交、多次回交和自交结合分子标记辅助选择技术，将Gm6基因导入广恢998中。综合目标基因的分子检测、田间农艺性状表现以及遗传背景的回复率，筛选获得6个株系。采用稻瘿蚊生物型1和生物型4对这些改良株系进行人工接虫鉴定，6个改良株系的标葱率为0，表现免疫，而对照标葱率均在98%以上，表现高感；由改良株系组配的杂种F1标葱率在10%~20%，表现中抗。农艺性状分析表明，除1个株系在千粒重方面与对照广恢998有显著差异外，其余改良株系性状与对照无显著差异。

关键词： 稻瘿蚊抗性; 恢复系; 分子标记辅助选择; 水稻; 育种

本文引用格式

柳武革,王丰,肖汉祥,刘振荣,李金华,廖亦龙, 朱满山,黄慧君,黄炳超,张扬 . 利用分子标记辅助选择改良水稻恢复系的稻瘿蚊抗性[J]. 中国水稻科学, 2010 , 24(6) : 581 -586 . DOI: 10.3969/j.issn.1001-7216.2010.06.004

Abstract

Gm6, a dominant gene conferring resistance to the four biotypes of Asian rice gall midge (Orseolia oryzae WoodMansion) occurred in South China from the donor KG18, was introduced into the restorer line Guanghui 998 with good grain quality and poor gall midge resistance, by using backcross breeding and molecular markerassisted selection. Six elite improved lines were developed based on the results of molecular markerassisted analysis, the recovery ratio of genetic background and the performance of agronomic traits. Evaluation of resistance to Chinese gall midge biotype 1 and biotype 4 was carried out. All the improved lines were immune to the pest with the ratio of silver shoots of 0 whereas the check Guanghui 998 were highly susceptible with the ratio of silver shoots of 98%. The hybrid F1, derived from the improved lines, showed moderate resistance against gall midge with the ratios of silver shoots ranging from 10% to 20%. The investigation on agronomic traits of the improved lines showed that there was no significant difference between the improved lines and the check except for 1000grain weight between one line and the check Guanghui 998. The improved lines could be used for breeding hybrid rice with higher gall midge resistance.

Key words： rice gall midge resistance; restorer line; molecular markerassisted selection; rice; breeding

参考文献

[1]谭玉娟, 黄炳超, 张扬, 等.中国抗稻瘿蚊育种研究进展//中国有害生物综合防治会议论文集. 北京：中国农业出版社, 1996: 345-350.

[2]黄炳超, 肖汉祥, 吕利华, 等. 抗稻瘿蚊新基因Gm6在分子标记抗性育种中的应用. 中国农业科学, 2004, 37(1): 76-80.

[3]黎国涛, 谭玉娟, 潘英, 等. 稻瘿蚊生物型的研究. 植物保护学报, 1983, 10(3): 147-152.

[4]谭玉娟, 张扬, 黄炳超, 等. 抗稻瘿蚊品种多抗1号的抗性遗传分析及抗性基因定位. 植物保护学报, 1996, 23(4): 315-320.

[5]Katiyar S K, Tan Y, Huang B, et al. Molecular mapping of gene Gm-6(t) which confers resistance against four biotypes of Asian rice gall midge in China. Theor Appl Genet, 2001, 103: 953-961.

[6]黄朝锋, 张桂权. 水稻PSM标记的发展及抗虫基因的分子定位. 分子植物育种, 2003, 1(4): 572-574.

[7]黄炳超, 张扬, 谢振文, 等. 华南抗稻瘿蚊分子标记辅助育种. 分子植物育种, 2007, 5(4): 507-514.

[8]黄炳超, 张扬, 谢振文, 等. 亚洲稻瘿蚊种群DNA指纹及水稻抗稻瘿蚊分子标记抗性育种技术研究. 昆虫知识, 2007, 44(2): 164-171.

[9]肖汉祥, 黄炳超, 张扬, 等. 与Gm6基因紧密连锁的PSM标记在水稻抗性育种中的应用.广东农业科学, 2005(3): 50-53.

[10]卢扬江, 郑康乐. 提取水稻DNA的一种简易方法. 中国水稻科学, 1992, 6(1): 47-48.

[11]Panaud O, Chen X, McCouch S R. Development of microsatellite markers and characterization of simple sequence length polymorphism(SSLP) in rice(O.sativa L.). Mol Gen Genet, 1996, 252: 597-607.

[12]McCouch S R, Teytelman L, Xu Y B, et al. Development and mapping of 2240 new SSR markers for rice (O.sativa L.). DNA Res, 2002, 9: 199-207.

[13]Hospital F, Chevalet C, Mulsant P. Using markers in gene introgression breeding programs. Genetics, 1992, 132:1199-1210.

[14]谢振文, 赵丽霞, 张扬, 等. 水稻抗稻瘿蚊基因遗传与抗虫育种技术. 广东农业科学, 1999(增): 31-32.

[15]唐启义, 冯明光. 实用统计分析及其DPS数据处理系统. 北京: 科学出版社, 2002: 53-55.

[16]黄显波, 邓则勤, 郑家团, 等. 抗稻瘿蚊和稻瘟病恢复系双抗名占的选育及抗性分析. 福建农业学报, 2007, 22(1): 1-4.

[17]彭炳生, 刘名镇, 鄢祖林, 等. 高抗稻瘿蚊两系杂交晚稻新组合安两优青占. 杂交水稻, 2004, 19(5): 66-67.

[18]郑家团，涂诗航，张建福, 等. 含白叶枯病抗性基因Xa23水稻恢复系的分子标记辅助选育. 中国水稻科学, 2009, 23(4): 437-439.

[19]柳武革, 王丰, 金素娟, 等. 利用分子标记辅助选择聚合Pi-1和Pi-2基因改良两系不育系稻瘟病抗性. 作物学报, 2008, 34(7): 1128-1136.

[20]Katiyar S, Verulkar S, Chandel G, et al. Genetic analysis and pyramiding of two gall midge resistance genes (Gm-2 and Gm-6t) in rice (Oryza sativa L.). Euphytica, 2001, 122: 327-334.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献