研究简报

旱稻品种IRAT109辐照诱变突变体的筛选和鉴定

展开
  • 上海市农业生物基因中心, 上海 201106; #共同第一作者; *通讯联系人, E-mail: hmei@sagc.org.cn

收稿日期: 1900-01-01

  修回日期: 1900-01-01

  网络出版日期: 2010-09-10

evelopment and Identification of Mutants Induced by γray Irradiation from an Upland Rice Variety IRAT109

Expand
  • Shanghai Agrobiological Gene Center, Shanghai 201106, China; # These authors contributed equally to this paper; *Corresponding author, E-mail: hmei@sagc.org.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-09-10

摘要

利用γ射线辐照处理旱稻品种IRAT109的干种子样品,加代至M2后混收种子,分批在水田和干旱胁迫条件下种植M3群体,通过苗期、分蘖期和抽穗期的观察获得突变单株,加代繁育至M5后考查可稳定遗传的突变性状,共获得260份不同性状的突变体。介绍了其中部分突变体在叶片、茎秆、穗部、籽粒形态和抗旱性等方面表现,配制个别突变体与野生型的F2群体并初步观察分离比例。讨论了筛选抗旱性相关突变体的技术困难和该套突变体群体的应用价值。

本文引用格式

邓姗,李华娟,冯芳君,徐小艳,黄志诚, 罗利军,梅捍卫 . 旱稻品种IRAT109辐照诱变突变体的筛选和鉴定[J]. 中国水稻科学, 2010 , 24(5) : 523 -526 . DOI: 10.3969/j.issn.1001-7216.2010.05.013

Abstract

Dry seed samples of the upland rice variety IRAT109 were treated with γray irradiation. Mixed seed bulk was collected from M2 plants. Mutated individuals were collected at seedling, tillering and heading stages from M3 population under dry cultivation and traditional cultivation for several seasons. The mutant population had 260 lines with stablyinherited mutations identified in M5 generation. A proportion of the mutants were described including mutants in leaves, culms, panicles, grains, and drought tolerancerelated traits. The segregation ratios of F2 population were observed for a couple of mutants. In addtion, the problems on screening mutations for drought tolerance and the utility of this mutant population were discussed.

参考文献

[1]Kurata N, Miyoshi K, Nonomura K I, et al. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 2005, 46: 48-62.
[2]Zhang J, Li C, Wu C, et al. RMD: A rice mutant database for functional analysis of the rice genome. Nucl Acids Res, 2006, 34: 745-748.
[3]肖景华, 吴昌银, 韩斌, 等. 中国水稻功能基因组研究进展. 中国科学: C辑, 2009, 39: 909-924.
[4]Krishnan A, Guiderdoni E, An G, et al. Mutant resources in rice for functional genomics of the grasses. Plant Physiol, 2009, 149: 165-170.
[5]Wu J L, Wu C, Lei C, et al. Chemical- and irradiation-induced mutants of indica rice IR64 for forward and reverse genetics. Plant Mol Biol, 2005, 59: 85-97.
[6]朱旭东, 陈红旗, 罗达, 等. 水稻中花11辐射突变体的分离与鉴定. 中国水稻科学, 2003, 17(3): 205-210.
[7]叶俊, 吴建国, 杜婧, 等. 水稻“9311”突变体筛选和突变体库构建. 作物学报, 2006, 32(10): 1525-1529.
[8]鲍根良, 左晓旭, 王俊敏, 等. 粳稻耐旱突变体的诱发与鉴定. 核农学报, 2005, 19(5): 332-335.
[9]Xiong L Z. Gene discovery for improving drought resistance of irrigated rice by systematic genetic and functional genomics approaches//Serraj J, Bennett J, Hardy B. Drought Frontiers in Rice: Crop Improvement for Increased Rainfed Production. Singapore: World Scientific Publishing and IRRI, 2008.
[10]高康宁, 张建林, 王化琪. 旱稻干旱敏感突变体的筛选和鉴定. 中国农业大学学报, 2009, 14: 35-40.
[11]张灿军, 姚宇卿, 王育红, 等. 旱稻抗旱性鉴定方法与指标研究: Ⅰ. 鉴定方法与评价指标. 干旱地区农业研究, 2005, 23(3): 33-36.
[12]Qi J, Qian Q, Bu Q Y, et al. Mutation of the rice NARROW LEAF 1 gene, which encodes a novel protein, affects vein patterning and polar auxin transport. Plant Physiol, 2008, 147: 1947-1959.
[13]Fujino K, Matsuda Y, Ozawa K, et al. NARROW LEAF 7 controls leaf shape mediated by auxin in rice. Mol Genet Genomics, 2008, 279: 499-507.
[14]汪得凯, 刘合芹, 李克磊, 等. 一个水稻窄叶突变体的鉴定和基因定位. 科学通报, 2009, 54: 360-365.
[15]李楠, 桑贤春, 赵芳明, 等. 水稻窄叶突变体nal(t)的表型分析与基因初步定位. 植物学报, 2010, 45(2): 157-161.
文章导航

/

浙ICP备05004719号-5
公安备案号:33010302003356
地址:浙江省杭州市富阳区水稻所路28号 邮编:311400 电话:0571-63370278 E-mail:cjrs@263.net
本系统由北京玛格泰克科技发展有限公司设计开发
总访问量: 今日访问: 在线人数: