水稻条纹病毒NS3蛋白与水稻 3-磷酸甘油醛脱氢酶（GAPDH）

肖冬来,贾东升,吴建国,杜振国,谢荔岩,吴祖建,谢联辉

doi:10.3969/j.issn.1001-7216.2010.05.008

中国水稻科学 >

2010 , Vol. 24 >Issue 5: 493 - 496

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.05.008

研究报告

水稻条纹病毒NS3蛋白与水稻 3-磷酸甘油醛脱氢酶（GAPDH）

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福建农林大学植物病毒研究所/福建省植物病毒学重点实验室，福建福州 350002; *通讯联系人， E-mail: wuzujian@126.com; fjxlh@126.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-09-10

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Interaction Between Rice Stripe Virus NS3 Protein and Glyceraldehyde-3-Phosphate Dehydrogenase(GAPDH) of Rice

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Key Laboratory of Plant Virology of Fujian Province/Institute of Plant Virology, Fujian Agriculture and Forestry University, Fuzhou 350002, China; *Corresponding author, E-mail: wuzujian@126.com; fjxlh@126.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-09-10

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摘要

水稻条纹病毒（rice stripe virus，RSV）NS3蛋白为病毒的沉默抑制子。利用酵母双杂交技术，从水稻cDNA文库中筛选出一个与RSV NS3蛋白互作的基因片段。推测该基因的功能是编码水稻3磷酸甘油醛脱氢酶（glyceraldehyde-3-phosphate dehydrogenase，GAPDH）。双分子荧光互补实验进一步验证了NS3与GAPDH存在互作。瞬时表达实验表明，GAPDH与GFP基因融合蛋白在本氏烟表皮细胞细胞质中大量积累。此外，讨论了GAPDH蛋白在RSV侵染水稻过程中可能的功能。

关键词： 水稻条纹病毒; 蛋白功能; 互作; 酵母双杂交; 双分子荧光互补

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肖冬来,贾东升,吴建国,杜振国,谢荔岩,吴祖建,谢联辉 .

水稻条纹病毒NS3蛋白与水稻 3-磷酸甘油醛脱氢酶（GAPDH）

[J]. 中国水稻科学, 2010 , 24(5) : 493 -496 . DOI: 10.3969/j.issn.1001-7216.2010.05.008

Abstract

NS3 protein of rice stripe virus (RSV) is an RNA silencing suppressor. Yeast twohybrid assay was used to screen the rice cDNA library with the NS3 protein as a bait. A novel rice protein interacting with RSV NS3 was obtained. The putative function of the interacting protein was glyceraldehyde3phosphate dehydrogenase (GAPDH). Bimolecular fluorescence complementation (BiFC) also showed that RSV NS3 could interact with the GAPDH. Cellular localization studies showed that GAPDHGFP fusion protein accumulated predominantly in cytoplasm of tobacco (Nicotiana benthamiana) through transient expression assay. Possible functions of GAPDH during the infection of rice by RSV were discussed.

Key words： rice stripe virus; protein function; interaction; yeast two hybrid; bimolecular fluorescence complementation

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