<FONT face="Verdana">水稻光温敏雄性核不育系广占63S不育基因<EM>PTGMS2-1</EM>的遗传分析与分子定位</FONT>

王宝和,徐建军,吴银慧,朱金燕,李生强, 周勇, 程小涛, 梁国华,

doi:10.3969/j.issn.1001-7216.2010.04.016

中国水稻科学 >

2010 , Vol. 24 >Issue 4: 429 - 432

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.04.016

研究简报

水稻光温敏雄性核不育系广占63S不育基因PTGMS2-1的遗传分析与分子定位

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¹扬州大学江苏省作物遗传生理国家重点实验室培育点/植物功能基因组学教育部重点实验室，江苏扬州 225009； ²江苏里下河地区农业科学研究所，江苏扬州 225007； *通讯联系人， E-mail: ricegb@yzu.edu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-07-10

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Genetic Analysis and Molecular Mapping of a PhotoperiodThermoSensitive Genic Male Sterile Gene (PTGMS2-1) in Rice Line Guangzhan 63S

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¹Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Function Genomics, Ministry of Education, Yangzhou University, Yangzhou 225009, China; ²Lixiahe Region Agricultural Research Institute of Jiangsu, Yangzhou 225007,China; *Corresponding author, E-mail: ricegb@yzu.edu.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-07-10

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摘要

水稻光温敏雄性核不育系广占63S的不育基因来源于光敏核不育系农垦58S，其育性转换表现为温敏型，是迄今两系杂交水稻中应用最广泛的籼型光温敏核不育系之一。对广占63S/旱1587组合的F₂分离群体进行不育基因的遗传分析，结果表明广占63S的不育性受1对隐性不育基因控制。利用SSR和InDel分子标记技术，结合BSA法，以广占63S与旱1587组合的F2分离群体为材料，将不育基因（暂命名为PTGMS2-1）定位于第2染色体标记S2-4与S2-27之间物理距离390 kb的区间内，与两标记间的遗传距离分别为0.5和1.1 cM，与标记S2-24共分离。

关键词： 光温敏雄性不育性; 遗传分析; 分子定位

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王宝和,徐建军,吴银慧,朱金燕,李生强, 周勇, 程小涛, 梁国华, . 水稻光温敏雄性核不育系广占63S不育基因PTGMS2-1的遗传分析与分子定位[J]. 中国水稻科学, 2010 , 24(4) : 429 -432 . DOI: 10.3969/j.issn.1001-7216.2010.04.016

Abstract

Guangzhan 63S, a photoperiodthermosensitive genic male sterile (PTGMS) line, was one of the most widely used PTGMS lines in twoline indica hybrid rice breeding system in China. Genetic analysis indicated that the sterility of Guangzhan 63S was controlled by a single recessive gene using the F2 population derived from a cross between the Guangzhan 63S and Han 1587. Molecular mapping was performed on the F2 mapping population using SSR and InDel markers and bulkedsegregant analysis(BSA). The PTGMS21 was restricted to a 390kb region on chromosome 2 between the markers S24 and S227, at genetic distances of 0.5 cM and 1.1 cM, respectively, and cosegregated with S224.

Key words： photoperiodthermosensitive genic male sterility; genetic analysis; molecular mapping

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