实验技术

灰飞虱从冷冻病叶获得水稻黑条矮缩病毒方法的研究初报

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  • 江苏省农业科学院 植物保护研究所, 江苏 南京 210014; #共同第一作者; *通讯联系人, E-mail: yjzhou@jaas.ac.cn

收稿日期: 1900-01-01

  修回日期: 1900-01-01

  网络出版日期: 2010-07-10

Preliminary Report on the Transmission of Rice BlackStreaked Dwarf Virus from Frozen Infected Leaves to Rice by Insect Vector Small Brown Planthopper (Laodelphax striatellus

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  • Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: yjzhou@jaas.ac.cn

Received date: 1900-01-01

  Revised date: 1900-01-01

  Online published: 2010-07-10

摘要

研究出一种利用灰飞虱从冷冻病叶中获得水稻黑条矮缩病毒(rice blackstreaked dwarf virus, RBSDV)的方法。以-70℃条件下冷冻保存的RBSDV罹病植株叶片对灰飞虱饲毒,随后接种感病水稻品种,结果发现灰飞虱可从冷冻病叶上获得RBSDV,并传播RBSDV,且获毒能力和传毒能力与新鲜病叶没有差异。这表明从冷冻病叶上获得RBSDV的灰飞虱可以应用于品种抗性鉴定,此法可望加快RBSDV抗病品种的选育进程。

本文引用格式

周彤,吴丽娟,王英,程兆榜,季英华,范永坚,周益军 . 灰飞虱从冷冻病叶获得水稻黑条矮缩病毒方法的研究初报[J]. 中国水稻科学, 2010 , 24(4) : 425 -428 . DOI: 10.3969/j.issn.1001-7216.2010.04.015

Abstract

In order to preserve virus for identifying resistant rice varieties against rice blackstreaked dwarf disease, a simple and reliable method was developed by which virusfree small brown planthopper(SBPH) acquired rice blackstreaked dwarf virus(RBSDV) from frozen infected leaves and transmitted the virus to healthy rice plants. RBSDV acquired from infected leaves frozen at -70℃ by SBPH was transmitted to a susceptible rice variety. The experimental result showed that the SBPH could acquire the virus from frozen infected leaves and transmit it to the susceptible rice variety. For the ability of obtaining and transmitting the virus by SBPH, there was no significant difference between frozen infected leaves and fresh infected leaves. The novel method could be applied to the resistance identification of rice varieties to rice blackstreaked dwarf disease and it would facilitate the breeding process for rice blackstreaked dwarf disease resistance.

参考文献

[1]朱凤美, 肖庆璞, 王法明, 等.江南稻区新发生的几种稻病.植物保护, 1964, 2(3): 100-102.
[2]陈声祥, 张巧艳.我国水稻黑条矮缩病与玉米粗缩病研究进展.植物保护学报, 2005, 32(1): 97-103.
[3]张恒木, 陈剑平, 薛庆中, 等.水稻黑条矮缩病毒基因组片段S10的cDNA克隆及全序列分析.中国水稻科学, 2002, 16(1): 24-28.
[4]王朝辉, 周益军, 范永坚, 等.江苏水稻黑条矮缩病毒S10的cDNA克隆序列分析.中国病毒学, 2002, 17(2): 142-144.
[5]Liu H J, Wei C H, Zhong Y W, et al.Rice black-streaked dwarf virus minor core protein P8 is a nuclear dimeric protein and represses transcription in tobacco protoplasts.FEBS Lett, 2007, 581(13): 2534-2540.
[6]阮义理, 金登迪, 许如银, 等.水稻黑条矮缩病的研究.浙江农业科学, 1984(4): 185-192.
[7]周益军, 范永坚, 程兆榜, 等.江苏省玉米病毒病研究: Ⅰ.玉米粗缩病的发生与病原初步鉴定.江苏农业学报, 1998, 14 (4): 246-248.
[8]羊健, 天慧, 戴良英, 等.我国水稻黑条矮缩病和玉米粗缩病病原的研究概况.江西植保, 2007, 30(1): 2-6.
[9]张恒木, 陈剑平, 雷娟丽, 等.水稻黑条矮缩病毒基因组片段S7的cDNA克隆及全序列分析.微生物学报, 2002, 42(2): 200-207.
[10]周彤, 王磊, 程兆榜, 等.主栽品种镇稻88对水稻条纹叶枯病的抗性特征及其遗传研究. 中国农业科学, 2009, 42(1): 103-109.
[11]孙黛珍, 江玲, 张迎信, 等.8个水稻品种的条纹叶枯病抗性特征.中国水稻科学, 2006, 20(2): 219-222.
[12]Hibino H.Biology and epidemiology of rice viruses.Annu Rev Phytopathol, 1996, 34: 249-274.
[13]Zhang S X, Li L, Wang X F, et al.Transmission of rice stripe virus acquired from frozen infected leaves by the small brown planthopper (Laodelphax striatellus Fallen).J Virol Methods, 2007, 146: 359-362.
[14]龚祖埙, 沈菊英, 陈巽祯, 等.我国禾谷类病毒病的病原问题: Ⅷ.玉米粗缩病病原的研究.生物化学与生物物理学报, 1981, 13(1): 55-59.
[15]张恒木, 陈剑平, 程晔, 等.水稻黑条矮缩病毒基因组片段S9的cDNA克隆和全序列分析.生物化学与生物物理学报, 2001, 33(1): 467-471.
[16]周益军, 刘海建, 王贵珍, 等.灰飞虱携带的水稻条纹病毒免疫检测.江苏农业科学, 2004(1): 50-51.
[17]陈洁, 吴丽娋, 周彤, 等.主栽抗病粳稻品种对水稻条纹叶枯病的抗性特征. 南京农业大学学报,2010, 33(4):
[18]孙黛珍, 江玲, 张迎信, 等.水稻抗条纹叶枯病数量性状座位分析.中国水稻科学, 2007, 21(1): 95-98.
[19]Shikata E, Kitagawa Y. Rice black-streaked dwarf virus: Its properties, morphology and intracellular localization. Virology, 1977, 77(2): 826-842.
[20]熊克娟, 李天宪, 陈绳亮, 等.常见植物病毒冷冻干燥方法的改进与效果观察.华中农业大学学报, 1999, 18(2): 151-153.
[21]Hollings M, Stone M.The long term survival of some plant viruses preserved by lyophilization. Ann Appl Boil, 1970, 65: 411-418.
[22]Hollings M, Lelliott R A.Preservation of plant viruses by freeze drying.Plant Pathol, 1960, 9: 63-66.
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