<FONT face="Verdana">高温对水稻灌浆期籽粒氮代谢关键酶活性及蛋白质含量的影响 </FONT>

梁成刚, 陈利平, 汪燕, 刘佳,许光利,李天

doi:10.3969/j.issn.1001-7216.2010.04.011

中国水稻科学 >

2010 , Vol. 24 >Issue 4: 398 - 402

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.04.011

研究报告

高温对水稻灌浆期籽粒氮代谢关键酶活性及蛋白质含量的影响

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四川农业大学农学院，四川雅安 625014； *通讯联系人， E-mail： lghhlt@hotmail.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-07-10

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Effects of High Temperature on Key Enzyme Activities of Nitrogen Metabolism and Protein Content During Rice Grain Filling

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Agronomy College, Sichuan Agricultural University, Ya’an 625014, China; *Corresponding author, E-mail: lghhlt@hotmail.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-07-10

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摘要

选用粳稻品种越光与籼稻品种IR72为材料，研究了高温对水稻灌浆期籽粒谷氨酰胺合成酶（GS）、谷氨酸合酶（GOGAT）、谷氨酸草酰乙酸转氨酶（GOT）、谷氨酸丙酮酸转氨酶（GPT）的活性及蛋白质与氨基酸含量的影响。结果表明，灌浆期高温下水稻籽粒GOGAT、GOT与GPT活性显著或极显著升高，但籽粒GS活性显著或极显著降低；籽粒蛋白质及氨基酸各组分含量极显著增加。高温下籽粒GS活性显著降低并未影响籽粒蛋白质的合成，认为籽粒GS不是限制水稻蛋白质合成的关键酶。

关键词： 高温; 氮代谢; 关键酶; 蛋白质含量; 氨基酸组分

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梁成刚, 陈利平, 汪燕, 刘佳,许光利,李天 . 高温对水稻灌浆期籽粒氮代谢关键酶活性及蛋白质含量的影响 [J]. 中国水稻科学, 2010 , 24(4) : 398 -402 . DOI: 10.3969/j.issn.1001-7216.2010.04.011

Abstract

The japonica rice variety Koshihikari and indica rice variety IR72 were used to research the effects of high temperature on the activities of glutamine synthetase(GS), glutamate synthase(GOGAT), glutamic oxaloacetic transaminase(GOT), glutamate pyruvate transaminase(GPT), and the contents of protein and amino acids in grains during rice grain filling. The average activities of GOGAT, GOT and GPT in grains significantly increased under high temperature during rice grain filling, while the activity of GS significantly decreased. And the contents of protein and amino acids constituents in grains significantly increased. The significant decrease of GS activity in grains under high temperature did not influence the synthesis of protein, so it is presumed that GS in rice grains was not the key enzyme to restrain the synthesis of protein.

Key words： high temperature; nitrogen metabolism; key enzyme; protein content; amino acids composition

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