<FONT face="Verdana">贵州地方水稻品种的SSR遗传多样性分析</FONT>

马琳,余显权,赵福胜

doi:10.3969/j.issn.1001-7216.2010.03.005

中国水稻科学 >

2010 , Vol. 24 >Issue 3: 237 - 243

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.03.005

研究报告

贵州地方水稻品种的SSR遗传多样性分析

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¹贵州大学生命科学学院，贵州贵阳 550025； ²贵州大学教学实验场，贵州贵阳 550025； ³贵州大学水稻研究所，贵州贵阳550025; *通讯联系人， E-mail: yxqgy@126.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-05-10

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SSR-Based Analysis on Genetic Diversity of Rice Landraces from Guizhou Province, China

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¹College of Life Sciences, Guizhou University, Guiyang 550025, China; ^2Teaching Experiment Farm, Guizhou University, Guiyang 550025, China; ³Rice Research Institute, Guizhou University, Guiyang 550025, China; *Corresponding author, E-mail: yxqgy@126.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-05-10

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摘要

利用21对SSR引物对74份贵州“禾”水稻品种及6份国际上常用的典型籼稻和粳稻品种进行遗传多样性研究。共检测到92个等位基因，每个位点的等位基因数变幅为2～9个，平均4.381个；平均Shannon信息指数为0.935，变幅为02793～1.8732；期望杂合度为0.5145，范围在0.0988～0.8313；品种间遗传相似系数为0.64～0.98。UPGMA聚类分析表明，在相似系数为0.682处可将“禾”品种分为4大类，大部分材料被聚类到典型粳稻的附近，品种间的亲缘关系与地理来源关系不大；主坐标分析结果与UPGMA聚类结果基本吻合。SSR检测结果表明，贵州省水稻地方品种——“禾”的多样性程度较低，且大多数属于粳稻。

关键词： 水稻; 地方品种; 微卫星标记; 遗传多样性; 主坐标分析

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马琳,余显权,赵福胜 . 贵州地方水稻品种的SSR遗传多样性分析[J]. 中国水稻科学, 2010 , 24(3) : 237 -243 . DOI: 10.3969/j.issn.1001-7216.2010.03.005

Abstract

The genetic diversity of 74 rice landraces from Guizhou Province, China was analyzed with 21 SSR markers and six standard varieties representing typical indica and japonica ecotypes. Ninetytwo alleles were detected. The average number of alleles per locus was 4.381 with a range from 2 to 9. The Shannon′s information indices varied widely from 02793 to 1.8732 with an average value of 0.935. The expected heterozygosities ranged from 0.0988 to 0.8313 with an average value of 0.5145. Cluster analysis results showed that the similarity coefficients varied from 0.64 and 0.98 and UPGMA cluster analysis showed that the rice materials tested could be divided into four groups at a genetic similarity coefficient of 0.682. Most of the materials were scattered around typical japonica ecotypes and the genetic relationship among these landraces was not in accord with their geographical origins. The results of principal coordinates analysis and UPGMA cluster analysis revealed that the genetic structure was multiplex in the rice landraces. The rice landraces from Guizhou Province showed lower genetic diversity and most were japonica ecotypes according to SSR analysis.

Key words： rice; landrace; simple sequence repeats; genetic diversity; principal coordinates analysis

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