利用染色体片段置换系定位水稻芽期耐冷性QTL

林静,朱文银,张亚东,朱镇,赵凌,陈涛,赵庆勇,周丽慧,方先文,王艳平,王才林

doi:10.3969/j.issn.1001-7216.2010.03.004

中国水稻科学 >

2010 , Vol. 24 >Issue 3: 233 - 236

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.03.004

研究报告

利用染色体片段置换系定位水稻芽期耐冷性QTL

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江苏省农业科学院粮食作物研究所/江苏省优质水稻工程技术研究中心/国家水稻改良中心南京分中心/江苏省农业种质资源保护与利用研究中心，江苏南京 210014； *通讯联系人， E-mail: clwang@jaas.ac.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-05-10

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Detection of Quantitative Trait Loci for Cold Tolerance at the Bud Bursting Stage by Using Chromosome Segment Substitution Lines in Rice (Oryza sativa)

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Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/Jiangsu High Quality Rice R & D Center/ Nanjing Branch of Chinese National Center for Rice Improvement/Research Center for Protection and Utilization of Agricultural Germplasm Resource in Jiangsu Province, Nanjing 210014, China; *Corresponding author, E-mail: clwang@jaas.ac.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-05-10

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摘要

以籼稻品种9311为受体、粳稻品种日本晴为供体构建的95个染色体片段置换系为材料，在5℃低温条件下进行芽期耐冷性鉴定。结果表明，6个置换系低温处理后的成苗率与受体亲本9311有一定差异，其耐冷性略强于9311。利用代换作图法共鉴定出4个与芽期耐冷性相关的QTL，分别位于水稻第5和第7染色体上。其中qCTB51、qCTB52和qCTB53分别被定位在第5染色体RM267与RM1237、RM2422与RM6054及RM3321与RM1054之间遗传距离分别为21.3 cM、27.4 cM和12.7 cM的置换片段上;qCTB7被定位在第7染色体RM11-RM2752区间遗传距离为6.8 cM的置换片段上。

关键词： 染色体片段置换系; 芽期耐冷性; 代换作图; 水稻

本文引用格式

林静,朱文银,张亚东,朱镇,赵凌,陈涛,赵庆勇,周丽慧,方先文,王艳平,王才林 .

利用染色体片段置换系定位水稻芽期耐冷性QTL

[J]. 中国水稻科学, 2010 , 24(3) : 233 -236 . DOI: 10.3969/j.issn.1001-7216.2010.03.004

Abstract

The cold tolerance at the bud bursting stage (CTB) were evaluated at 5℃ by using a set of 95 chromosome segment substitution lines (CSSLs) derived from indica rice 9311 and japonica rice Nipponbare. The results showed that six CSSLs had slightly stronger CTB than 9311. Total four quantitative trait loci (QTLs) for CTB were preliminary mapped on chromosomes 5 and 7 by substitution mapping. qCTB51, qCTB52 and qCTB53 were mapped in the region of RM267-RM1237,RM2422-RM6054 and RM3321-RM1054, which were 21.3 cM, 27.4 cM and 12.7 cM in genetic distance on rice chromosome 5, respectively. qCTB7 was mapped in the region of RM11-RM2752, which was 6.8 cM in genetic distance on rice chromosome 7.

Key words： chromosome segment substitution lines; cold tolerance at the bud bursting stage; substitution mapping

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