<FONT face="Verdana">一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位</FONT>

李超,林冬枝,  ,董彦君叶胜海,张小明

doi:10.3969/j.issn.1001-7216.2010.03.002

中国水稻科学 >

2010 , Vol. 24 >Issue 3: 223 - 227

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.03.002

研究报告

一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位

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¹上海师范大学生命与环境科学学院植物遗传与功能基因研究室, 上海 200234; ²浙江省农业科学院作物与核技术利用研究所，浙江杭州 310021; *通讯联系人, E-mail: dzlin@shnu.edu.cn; dong@shnu.edu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-05-10

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Genetic Analysis and Mapping of a ThermoSensitive White Stripe Leaf Mutant at the Seedling Stage in Rice(Oryza sativa)

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¹Laboratory of Plant Genetics and Functional Genes, College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China; ²Institute of Crop and Nuclear Technology Utilization, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China; *Corresponding author, E-mail: dzlin@shnu.edu.cn; dong@shnu.edu.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-05-10

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摘要

通过对粳稻品种嘉花1号60Co γ射线诱变，从M2中筛选出一株低温敏感型白色条斑叶突变体(tws)。它在低温(20℃，24℃)条件下培养时，苗期第3和第4叶表现出白色条斑，而第5叶开始转为正常。低温条件下该突变体白斑叶片叶绿素含量明显下降。该突变体白色条斑叶性状具有温敏感性，且与叶龄相关。遗传分析表明，该突变性状受1对隐性核基因控制，定名为tws(thermosensitive white stripeleaf)基因。以tws突变体与籼稻9311杂交的F2分离群体作为定位群体，利用SSR标记将该基因定位在第4染色体MM3907和MM3928之间，其物理距离约为86 kb。

关键词： 水稻; 温度敏感性; 白色条斑叶

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李超,林冬枝, ,董彦君叶胜海,张小明 . 一个水稻苗期温敏感白色条斑叶突变体的遗传分析及基因定位[J]. 中国水稻科学, 2010 , 24(3) : 223 -227 . DOI: 10.3969/j.issn.1001-7216.2010.03.002

Abstract

A thermosensitive white stripeleaf mutant (tws) was selected from the M2 progeny of a japonica rice variety, Jiahua 1, treated with 60Co γradiation. In comparison with the wild type parent (Jiahua 1), the mutant displayed a phenotype of white stripe on the 3rd and 4th leaves, but began to turn a normal green phenotype on the 5th leaf when grown at low temperatures (20℃, 24℃). And also the content of total chlorophyll showed an obvious decrease in the leaves with white stripe. These results suggest that the expression of the mutant trait was thermosensitive and correlated with the leaf age of seedlings. The genetic analysis indicated that the mutant trait was controlled by a single recessive nuclear gene, designated as tws (thermosensitive white stripeleaf). In addition, by using SSR markers and an F2 segregating population derived from the cross between the tws mutant and 9311, the tws gene was mapped between MM3907 and MM3928 with a physical distance of 86 kb on chromosome 4.

Key words： rice(Oryza sativa); thermosensitivity; white stripeleaf; gene mapping

参考文献

[1]Han B, Xue Y B, Li J Y, et al. Rice functional genomics research in China. Phil Trans R Soc B, 2007, 362(1482): 1009-1021.

[2]Alberte R S, Hesketh J D, Hofstra G, et al. Composition and activity of the photosynthetic apparatus in temperature sensitive mutants of higher plants. Proc Natl Acad Sci USA, 1974, 71: 2414-2418.

[3]Markwell J, Osterman J C. Occurrence of temperature-sensitive phenotypic plasticity in chlorophyll-deficient mutants of Arabidopsis thaliana. Plant Physiol, 1992, 98: 392-394.

[4]Gálová E, Bhmová B, Sevcovicová A. Analysis of some barley chlorophyll mutants and their response to temperature stress. Photosynthetica, 2000, 38: 29-35.

[5]Soo C Y, Sung H C, Hiroki S, et al. Rice virescent3 and stripe1 encoding the large and small subunits of ribonucleotide reductase are required for chloroplast biogenesis during early leaf development. Plant Physiol, 2009, 150: 388-401.

[6]Pasini L, Bruschini S, Bertoli A, et al. Photosynthetic performance of cold-sensitive mutants of maize at low temperature. Physiol Plant, 2005, 124: 362-370.

[7]Dwyer L M, Tollenaar M, Houwing L. A nondestructive method to monitor leaf greenness in corn. Can J Plant Sci, 1991, 71: 505-509.

[8]Watanabe S, Hatanaka Y, Inada K. Development of a digital chlorophyll meter: Ⅰ. Structure and performance. Jpn J Crop Sci, 1980, 49(special issue): 89-90.

[9]Muray M G, Thompson W F. Rapid isolation of high molecular weight plant DNA. Nucl Acids Res, 1980, 8(19): 4321-4325.

[10]Lincoln S, Daly M, Lander E. Constructing genetics maps with MAPMAKER/EXP 3.0. Whitehead Institute Technical Report. 3rd ed. Cambridge, Massachusetts: Whitehead Institute, 1992.

[11]Kurata N, Miyoshi K, Nonomura K, et al. Rice mutants and genes related to organ development, morphogenesis and physiological traits. Plant Cell Physiol, 2005, 46: 48-62.

[12]黄晓群, 赵海新, 董春林, 等. 水稻叶绿素合成缺陷突变体及其生物学研究进展. 西北植物学报, 2005, 25(8): 1685-1691.

[13]Kusumi K, Mizutani A, Nishimura M, et al. A virescent gene V1 determines the expression timing of plastid genes for transcription/translation apparatus during early leaf development in rice. Plant J, 1997, 12(6): 1241-1250.

[14]Sugimoto H, Kusumi K, Tozawa Y, et al. The virescent 2 mutation inhibits translation of plastid transcripts for the plastid genetic system at an early stage of chloroplast differentiation. Plant & Cell Physiol, 2004, 45(8): 985-996.

[15]Hiroki S, Kensuke K, Ko N, et al. The rice nuclear gene, VIRESCENT 2, is essential for chloroplast development and encodes a novel type of guanylate kinase targeted to plastids and mitochondria. Plant J, 2007, 52: 512-527.

[16]董文其, 董彦君, 石守鋆, 等. 两用核不育水稻7436S的温敏感白苗标志性状的遗传及其育性研究. 作物品种资源, 1995(1): 19-21.

[17]Dong Y J, Dong W Q, Shi S Y, et al. Identification and genetic analysis of a thermo-sensitive seedling-colour mutant in rice (Oryza sativa L.). Breeding Sci, 2001, 51: 1-4.

[18]舒庆尧, 刘贵付, 夏英武. 温敏水稻叶色突变体的研究. 核农学报, 1996, 10(1): 6-10.

[19]Dong Y J, Shi S Y, Zhang H D, et al. Temperature response and inheritance of a thermo-sensitive seedling colour mutant in rice (Oryza sativa L.). J Genet & Breeding, 1994, 48: 423-426.

[20] 董彦君, 董文其, 张小明, 等. 突变体Fan 5苗色低温敏感性状的遗传分析. 中国水稻科学, 1995, 9(4): 249-250.

[21] 崔海瑞, 夏英武, 高明尉. 温度对水稻突变体W1叶色及叶绿素生物合成的影响. 核农学报, 2001, 15(5): 269-273.

[22] Xia J C, Wang Y P, Ma B T, et al. Ultrastructure and gene mapping of the albino mutant al12 in rice (Oryza sativa L.). Acta Genet Sin, 2006, 33(12): 1112-1119.

[23]Liu W Z, Fu Y P, Hu G C, et al. Identification and fine mapping of a thermo-sensitive chlorophyll deficient mutant in rice (Oryza sativa L.). Planta, 2007, 226: 785-795.

[24]李娜, 储黄伟, 文铁桥, 等. 水稻白色中脉Oswm突变体的遗传分析与基因定位. 上海农业学报, 2007, 23(1): 1-4.

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