<FONT face="Verdana">利用C0t-1 DNA对高秆野生稻和宽叶野生稻基因组的比较分析</FONT>

王德彬,王阳,赵侯明,李刚,覃瑞,刘虹

doi:10.3969/j.issn.1001-7216.2010.02.05

中国水稻科学 >

2010 , Vol. 24 >Issue 2: 131 - 136

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.02.05

研究报告

利用C0t-1 DNA对高秆野生稻和宽叶野生稻基因组的比较分析

展开

中南民族大学生命科学学院/生物技术国家民委重点实验室，湖北武汉 430074; *通讯联系人, E-mail: liuhong2007@yahoo.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-03-10

收起

Comparative Analysis of Genomes of Oryza alta and Oryza latifolia by Using C0t-1 DNA

Expand

Key Laboratory of State Ethnic Affairs Commission for Biological Technology/College of Life Sciences, SouthCentral University for Nationalities, Wuhan 430074, China；*Corresponding author, E-mail: liuhong2007@yahoo.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-03-10

Fold

摘要

为了揭示同为CCDD基因组类型的高秆野生稻和宽叶野生稻的起源和进化关系，用高秆野生稻C0t1 DNA作为探针，对其自身体细胞染色体与宽叶野生稻体细胞染色体进行荧光原位杂交并对其核型进行同源性聚类和比较分析。杂交结果显示宽叶野生稻和高秆野生稻同源性非常高，说明它们的亲缘关系十分接近，但区别也非常明显，两者的杂交信号存在显著不同。C0t1 DNA具有很强的种特异性和依赖基因组型的特异性，利用它进行荧光原位杂交能够更有效地研究物种基因组间的关系，为物种的划分和鉴定提供依据。另外，通过对高秆野生稻和宽叶野生稻两个异源四倍体基因组的中高度重复序列的比较分析，探讨了稻属异源四倍体可能的起源与进化机制。

关键词： 高秆野生稻; 宽叶野生稻; 染色体重复序列; 荧光原位杂交; 核型; 基因组; 亲缘关系

本文引用格式

王德彬,王阳,赵侯明,李刚,覃瑞,刘虹 . 利用C0t-1 DNA对高秆野生稻和宽叶野生稻基因组的比较分析[J]. 中国水稻科学, 2010 , 24(2) : 131 -136 . DOI: 10.3969/j.issn.1001-7216.2010.02.05

Abstract

In order to reveal the origin and evolutionary relationship between two CCDD genome species of rice, Oryza alta and Oryza latifolia, fluorescence in situ hybridization (FISH) procedure was adopted to analyse the genomes of O. alta and O. latifolia using C0t1 DNA from O. alta as a probe. Karyotype analysis between O. alta and O. latifolia was also made comparatively based on their similar band patterns of the hybridization signals. There was a high homology and close relationship between O. alta and O. latifolia, but at the same time, the distinction between the hybridization signals was also clear. This indicates that C0t1 DNA was species and genome typespecific, and C0t1 DNAFISH could be more efficient in analysis of the genome relationship between different species. According to the comparative analysis of highly and moderately repetitive DNA sequences between the two allotetraploidy species, O. alta and O.latifolia, the possible origin and evolutionary mechanism of allotetraploidy of Oryza were discussed.

Key words： Oryza alta; Oryza latifolia; chromosomal repeat sequence; fluorescence in situ hybridization; karyotype; genome; phylogenetic relation

参考文献

[1]Lu B R. Taxonomy of the genus Oryza (Poaceae): A historical perspective and current status. Int Rice Res Notes, 1999, 24: 4-8.

[2]Aggarwal R K, Brar D S, Khush G S. Two new genomes in the Oryza complex identified on the basic of molecular divergence analysis using total genomic DNA hybridization. Mol Gen Genet, 1997, 254: 1-12.

[3]Vaughan D A. The genus Oryza L. Current status of taxonomy. IRRI Research Paper Series No. 138. Manila: IRRI, 1989: 1-21.

[4]Vaughan D A, Morishima H, Kadowaki K. Diversity in the Oryza genus. Curr Opin Plant Biol, 2003, 6: 139-146.

[5]Ge S, Sang T, Lu B R, et al. Phylogeny of rice genomes with emphasis on origins of allotetraploid species. Proc Natl Acad Sci USA, 1999, 96: 14400-14405.

[6]Liu L, Lafitte R, Guan D. Wild Oryza species as potential sources of droughtadaptive traits. Euphytica, 2004, 138: 149-161.

[7]Jena K K, Kochert G. Restriction fragment length polymorphism analysis of CCDDgenome species of the genus Oryza L. Plant Mol Biol, 1991, 16: 831-839.

[8]Gopalakrishnan R, Sampath S. Taxonomic status and the origin of American tetraploid species of the series Latifoliae Tateoka in the genus Oryza. Indian J Agric Sci, 1967, 37: 465-475.

[9]Aggarwal R K, Brar D S, Nandi S, et al. Phylogenetic relationships among Oryza species revealed by AFLP markers. Theor Appl Genet, 1999, 98: 1320-1328.

[10]Bao Y, Ge S. Identification of Oryza species with the CD genome based on RFLP analysis of nuclear ribosomal ITS sequences. Acta Bot Sin, 2003, 45(7): 762-765.

[11]Bao Y, Ge S. Origin and phylogeny of Oryza species with the CD genome based on multiplegene sequence data. Plant Syst, 2004, 249: 55-66.

[12]蓝伟侦, 覃瑞, 李刚，等. 利用C基因组C0t1 DNA比较分析稻属A, B, C, D基因组. 科学通报, 2006, 51(12): 1422-1431.

[13]Flavell R B. Repetitive DNA and chromosome evolution in plants. Philos Trans R Soc Lond B: Biol Sci, 1986, 312: 227-242.

[14]Bennetzen J L, Ma J, Devos K M. Mechanisms of recent genome size variation in flowering plants. Ann Bot, 2005, 95: 127-132.

[15]Matyasek R, Gazdova B, Fajkus J, et al. NTRS, a new family of highly repetitive DNAs specific for the T1 chromosome of tobacco. Chromosoma, 1997, 106: 369-379.

[16]Zwick M S, Hanson R E, Mcknight T D, et al. A rapid procedure for the isolation of C0t1 DNA from plants. Genome, 1997, 40: 138-142.

[17]蓝伟侦, 何光存, 吴士筠, 等. 利用水稻C0t1 DNA和基因组DNA对栽培稻、药用野生稻和疣粒野生稻基因组的比较分析.中国农业科学, 2006, 39(6): 1083-1090.

[18]Ren N, Song Y C, Bi X Z, et al.The physical location of genes cdc2 and prh1 in maize (Zea mays L.). Hereditas, 1997, 126(3): 211-217.

[19]Jiang J M, Gill B S, Wang G L, et al. Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosome. Proc Natl Acad Sci USA, 1995, 92: 4487-4491.

[20]Wei W H, Qin R, Song Y C, et al. Comparative analyses to diseases resistant and nonresistant lines from maize Zea diploperennis by GISH. Bot Bull Acad Sin, 2001, 42: 109-114.

[21]Cheng Z K, Dong F G, Langdon T, et al. Functional rice centromeres are marked by a satellite repeat and a centromerespecific retrotransposon. Plant Cell, 2002, 14: 1691-1704.

[22]Ren F G, Lu B R, Li S Q, et al. A comparative study of genetic relationships among the AAgenome Oryza species using RAPD and SSR markers. Theor Appl Genet, 2003, 108: 113-120.

[23]Schwarzacher T, Leitch A R, Bennett M D, et al. In situ hybridization of parental genomes in a wide hybrid. Ann Bot, 1989, 64: 315-324.

[24]Fukui K, Shishido R, Kinoshita T. Identification of the rice D genome chromosomes by genomic in situ hybridization. Theor Appl Genet, 1997, 95: 1239-1245.

[25]Li C B, Zhang D M, Ge S, et al. Differentiation and inter genomic relationships among C, E and D genomes in the Oryza officinalis complex (Poaceae) as revealed by multicolor genomic in situ hybridization. Theor Appl Genet, 2001, 103:197-203.

[26]Sakurako U, Hiroshi I, Nobuko O, et al. Repetitive sequences: Cause for variation in genome size and chromosome morphology in the genus Oryza. Plant Mol Biol, 1997, 35: 791-799.

[27]Nobuko O, Kijima K, Akiyama Y, et al. Quantification of total genomic DNA and selected repetitive sequences reveals concurrent changes in different DNA families in indica and japonica rice. Mol Gen Genet, 2000, 263: 388-394.

[28]Toshie M, Ken L N, Hiroko M, et al. Genome size of twenty wild species of Oryza determined by flow cytometric and chromosome analyses. Breeding Sci, 2007, 57: 73-78.

[29]Second G, Rouhan G. Humanmediated emergence as a weed and invasive radiation in the wild of the CD genome allotetraploid rice species (Oryza, Poaceae)in the Neotropics. PLoS ONE, 2008, 3(7): e2613. doi:10.1371/journal.pone.0002613

[30]Yin P, Hartemink A J.Theoretical and practical advances in genome halving. Bioinformatics, 2005, 21: 869-879.

[31]Yogeeswaran K, Frary A, York T L, et al. Comparative genome analyses of Arabidopsis spp.: Inferring chromosomal rearrangement events in the evolutionary history of A. thaliana. Genome Res, 2005, 15: 505-515.

[32]Han F, Fedak G, Guo W, et al. Rapid and repeatable elimination of a parental genomespecific DNA repeat (pGc1R1a) in newly synthesized wheat allopolyploids. Genetics, 2005, 170: 1239-1245.

[33]Ma X F, Gustafson J P. Timing and rate of genome variation in triticale following allopolyploidization. Genome, 2006, 49: 950-958.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献