<FONT face="Verdana">红莲型不育细胞质在杂交粳稻育种中的应用 </FONT>

汤述翥,张宏根,朱正斌,刘超,李鹏,梁国华,严长杰,刘巧泉,于恒秀,顾铭洪

doi:10.3969/j.issn.1001-7216.2010.02.03

中国水稻科学 >

2010 , Vol. 24 >Issue 2: 116 - 124

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.02.03

研究报告

红莲型不育细胞质在杂交粳稻育种中的应用

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扬州大学江苏省作物遗传生理重点实验室/教育部植物功能基因组学重点实验室，江苏扬州 225009

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-03-10

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Application of HL Type Male Sterile Cytoplasm in Japonica Hybrid Rice Breeding

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Key Laboratory of Crop Genetics and Physiology of Jiangsu Province / Key Laboratory of Plant Functional Genomics， Ministry of Education， Yangzhou University， Yangzhou 225009， China

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-03-10

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摘要

为了探讨红莲型不育细胞质应用于杂交粳稻育种的可能性，利用包台（BT）型、红莲(HL)型、茶野（CL）型、野败（WA）型等4种细胞质的同核异质不育系六千辛A与181个粳稻亲本测交，根据测交F1的小穗育性，筛选HL型粳稻恢复系。在此基础上，对筛选出的HL型粳稻恢复系与更多HL型不育系复测，并进行不同细胞质杂交粳稻的比对试验。研究结果表明： 1）HL型和CL型不育系的恢、保关系十分一致，测交F1小穗育性间的相关性达极显著水平。2）HL型和CL型粳稻不育系的可恢性虽不如BT型不育系，但明显优于WA型粳稻不育系；以测交F1小穗育性达到85%以上作为选择标准，从BT型恢复系和广亲和恢复系中筛选出25个HL型（CL型）粳稻恢复系。3）不育系的核背景对杂种育性有影响，HL型六千辛A可恢性最好，其次为HL型陵香A，HL型珍5A可恢性最差。4）与BT型不育系配制的杂种相比， HL型和CL型不育系配制的杂种育性稳定性相对较差。5）HL型不育系配制的杂种与BT型、WA型不育系配制的杂种在播种至抽穗历期、株高、产量及品质性状上均无明显差异；HL型不育系配制的杂交粳稻结实率正常，与BT型不育系配制的杂种无明显差异，明显高于WA型不育系配制的杂种。说明HL型不育细胞质应用于杂交粳稻育种是可行的。

关键词： 杂交粳稻; 红莲型不育细胞质

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汤述翥,张宏根,朱正斌,刘超,李鹏,梁国华,严长杰,刘巧泉,于恒秀,顾铭洪 . 红莲型不育细胞质在杂交粳稻育种中的应用 [J]. 中国水稻科学, 2010 , 24(2) : 116 -124 . DOI: 10.3969/j.issn.1001-7216.2010.02.03

Abstract

For investigating the feasibility of applying HL type male sterile cytoplasm to japonica hybrid rice breeding, a set of isonuclear alloplasmic cytoplasmic male sterile(CMS) lines(A lines) of Liuqianxin, including four different cytoplasms from Indian Chinsurah BoroⅡ(BT type), Hainan red awned wild rice(HL type), Hunan Chaling wild rice(CL type) and Hainan male sterile wild rice (WA type), and the maintainer line(B line), were used to make testcross F1 with 181 japonica parents including BT type restorer lines(R lines), wide compatible restorer lines(WCR lines), wide compatible varieties(WCVs), Yunnan rice landraces and other japonica varieties/lines. According to the spikelet fertility of testcross F1, japonica R lines for HL type male sterile cytoplasm had been selected. Then, the selected japonica R lines were testcrossed with three HL type CMS lines(including Liuqianxin A, Lingxiang A and Zhen 5A) and used to make japonica hybrid rice combinations. The results showed that the HL type CMS line and CL type CMS line were most similar on restorermaintainer relationship with a significant correlation between the spikelet fertility of testcross F1 of HL Liuqianxin A and that of CL Liuqianxin A. The HL type and CL type japonica A lines showed worse restored ability than BT type CMS line, but better than WA type japonica CMS line. With the standard that the spikelet fertility of testcross F1 over 85%, 25 HL type (CL type) japonica R lines were selected from BT type R lines and WCR lines. The nuclear background of CMS lines affected the fertility of F1. As to the restored ability, HL Liuqianxin A was the best, HL Lingxiang A the second, HL Zhen 5A the worst. The spikelet fertilities of F1s derived from HL type and CL type CMS lines were less stable than that from BT type CMS line. Japonica hybrid rice combinations derived from HL type, BT type, and WA type CMS lines had no significant difference in days from sowing to heading, plant height, yield and rice quality traits. On seedsetting percentage, japonica hybrid rice combinations of HL Lingxiang A/SJR48221 and BT Lingxiang A/SJR48221 had no significant difference, but HL Zhen 5A/SJR48221 was higher than WA Zhen 5A/SJR48221. Accordingly, it is feasible to apply HL type male sterile cytoplasm in japonica hybrid rice breeding.

Key words： japonica hybrid rice; HL type male sterile cytoplasm; restorer line; breeding

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