<FONT face="Verdana">稻米直链淀粉含量的低世代筛选方法研究 </FONT>

王跃星,倪深,陈红旗,刘贵富,杨军,段彬伍,朱旭东,

doi:10.3969/j.issn.1001-7216.2010.01.16

中国水稻科学 >

2010 , Vol. 24 >Issue 1: 93 - 98

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.01.16

实验技术

稻米直链淀粉含量的低世代筛选方法研究

展开

1中国水稻研究所水稻生物学国家重点实验室，浙江杭州 310006； 2中国科学院遗传与发育生物学研究所，北京 100101； *通讯联系人， E-mail: ricezxd@126.com

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-01-10

收起

Improvement of Method for Evaluating Amylose Content in Rice at Low Generations of Breeding

Expand

1State Key Laboratory of Rice Biology, China National Rice Research Institute， Hangzhou 310006， China； 2Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; *Corresponding author, E-mail: ricezxd@126.com

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-01-10

Fold

摘要

针对稻米直链淀粉含量在育种低世代遗传改良中的问题，提出了简易的碘蓝染色法：采用一定量的半粒糙米染色，按照染色后颜色由棕红到深蓝的差异，将稻米直链淀粉含量分为4个类别，分别对应的直链淀粉含量由低到高。使用该方法测定了28份供试水稻品种，染色结果与实测值呈极显著相关（r = 0.95**）。利用低世代遗传群体的研究，并通过分子标记辅助验证了该方法对Wx基因筛选的准确性。22份恢复系与保持系育种亲本通过此法所得直链淀粉含量与稻米淀粉合成相关基因标记之间的符合率较高。

关键词： 水稻; 直链淀粉含量; 品质改良; 低世代; 染色法; 分子标记辅助选择; 育种

本文引用格式

王跃星,倪深,陈红旗,刘贵富,杨军,段彬伍,朱旭东, . 稻米直链淀粉含量的低世代筛选方法研究 [J]. 中国水稻科学, 2010 , 24(1) : 93 -98 . DOI: 10.3969/j.issn.1001-7216.2010.01.16

Abstract

A simple iodine staining method for evaluating amylose content in rice was developed. Amylose content in rice was divided into four levels from low to high, according to the colors from brown red to dark blue after staining. The evaluation on the amylose content in 28 rice varieties by using the method showed that there was a significant correlation between the staining results and standard value (r = 0.95**). The method was applied to the improvement of rice amylose content in low generations, and the accuracy of the method in Wx gene screening was verified by molecular marker assistant selection. The amylose content evaluated by this method was highly coincident with rice starch synthesisrelated gene markers in 22 breeding parents for restorer and maintainer lines of hybrid rice.

Key words： rice; amylose content; quality improvement; low generation; staining method; molecular marker assistant selection; breeding

参考文献

[1]中华人民共和国农业部部颁标准NY147-88.米质测定方法. 北京: 中国标准出版社, 1989.

[2]罗玉坤, 申岳正, 闵绍楷, 等. 单粒稻米直链淀粉含量测定方法的研究. 中国水稻科学, 1988, 2(3): 136-140.

[3]申岳正, 闵绍楷, 熊振民, 等. 稻米直链淀粉含量的遗传测定方法改进. 中国农业科学, 1990, 23(1): 60-68.

[4]黄超武, 李锐. 水稻杂种直链淀粉含量的遗传分析. 华南农业大学学报, 1990, 33(6): 1-5.

[5]何秀英, 甄海. 糙米直链淀粉单粒测定研究. 广东农业科学, 1999(1): 2-4.

[6]陈楚, 张云芳, 王守海. 单粒稻米直链淀粉含量测定法的改进. 安徽农业科学, 2005, 33(2): 196-197.

[7]杨金华, 毕晨光. 半粒稻米直链淀粉测定方法的研究及应用. 作物学报, 1992, 18(5): 366-371.

[8]王仪春, 张小明, 石春海. 稻米直链淀粉含量测定方法的探讨. 中国农学通报, 2001, 17(5): 30-32, 51.

[9]Bligh H F L, Till R I, Jones C A. A microsatellite sequence closely linked to the wx gene of Oryza sativa. Euphytica, 1995, 86: 83-85.

[10]Hirano H Y, Eiguchi M, Sano Y. A single base change altered the regulation of the waxy gene at the posttranscriptional level during the domestication of rice. Mol Biol Evol, 1998, 15: 978-987.

[11]Tan Y F, Zhang Q F. Correlation of simple sequence repeat (SSR) variants in the leader sequence of the wx gene with amylose content of the grain in rice. Acta Bot Sin, 2001, 43(2): 146-150.

[12]Yamanouchi H, Nakamura Y. Organ specificity of isoforms of starch branching enzyme (Qenzyme) in rice. Plant Cell Physiol, 1992, 33: 985-991.

[13]Mizumo K, Kawasaki T, Shimada H, et al. Alteration of the structural properties of starch components by the lack of an isoform of starch branching enzyme in rice seeds. J Biol Chem, 1993, 268: 19084-19091.

[14]李浩杰, 李平, 高方远, 等. SSR标记辅助选择改良冈46B直链淀粉含量的研究. 作物学报, 2004, 30(11): 1159-1163.

[15]张士陆, 倪大虎, 易成新, 等. 分子标记辅助选择降低籼稻057的直链淀粉含量. 中国水稻科学, 2005, 19(5): 467-470.

[16]刘巧泉, 蔡秀玲, 李钱峰, 等. 分子标记辅助选择改良特青及其杂交稻米的蒸煮与食味品质. 作物学报, 2006, 32(1): 64-69.

[17]陈圣, 倪大虎, 陆徐忠, 等. 利用分子标记技术降低协优57的直链淀粉含量. 中国水稻科学, 2008, 22(6): 597-602.

[18]黄超武, 李锐. 水稻直链淀粉含量的遗传分析//莫惠栋. 谷物作物品质性状遗传研究进展. 南京: 江苏科学技术出版社, 1990: 64-67.

[19]李欣, 顾铭洪, 潘学彪, 等. 稻米直链淀粉含量的遗传及选择效应的研究//莫惠栋. 谷物作物品质性状遗传研究进展. 南京: 江苏科学技术出版社, 1990: 68-74.

[20]胡培松, 翟虎渠, 唐绍清, 等. 稻米糊化温度和直链淀粉含量的简易测定法. 中国水稻科学, 2003, 17(3): 284-286.

Options

文章导航

模态框（Modal）标题

摘要

本文引用格式

Abstract

参考文献