<FONT face="Verdana">褐飞虱对氟虫腈和新烟碱类药剂的抗性动态变化</FONT>

刘叙杆,赵兴华,王彦华,韦锦捷,沈晋良,孔健,曹明章,周威君,罗才宏

doi:DOI: 10.3969/j.issn.1001-7216.2010.01.13

中国水稻科学 >

2010 , Vol. 24 >Issue 1: 73 - 80

DOI: https://doi.org/DOI: 10.3969/j.issn.1001-7216.2010.01.13

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褐飞虱对氟虫腈和新烟碱类药剂的抗性动态变化

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1南京农业大学植物保护学院农药科学系/农业部作物病虫害监测与防控重点开放实验室, 江苏南京 210095; 2 浙江省农业科学院农产品质量标准研究所，浙江杭州 310021； 3 深圳诺普信农化股份有限公司研究所，广东深圳 518102； ﹟共同第一作者； *通讯联系人, E-mail: jlshen@njau.edu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-01-10

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Dynamic Changes of Resistance to Fipronil and Neonicotinoid Insecticides in Brown Planthopper, Nilaparvata lugens(Homoptera: Delphacidae)

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1Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University/Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing 210095, China; 2Institute of Quality and Standard for Agro products, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China;3Institute of Shenzhen Noposion Agrochemical CO. Ltd.， Shenzhen 518102, China; #These authors contributed equally to this paper; *Corresponding author, E-mail: jlshen@njau.edu.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-01-10

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摘要

于2006-2008年采用稻茎浸渍法监测了我国主要水稻种植区大田褐飞虱种群对苯基吡唑类杀虫剂(氟虫腈)、新烟碱类及昆虫生长调节剂类杀虫剂(噻嗪酮)的抗性动态变化。结果表明，2008年褐飞虱对氟虫腈的抗性水平随其迁飞途径而出现急剧上升态势，其中，从我国南方稻区迁入长江中下游流域等6省10地种群和回迁至广东韶山、深圳两种群对氟虫腈抗性分别上升至中等水平抗性（15.0~32.5倍）和高水平抗性（66.9~73.7倍），明显高于2006年5省6地（2.6~5.8倍）和2007年9省11地（3.2~8.4倍）大田褐飞虱种群的抗性水平。上述结果预示2009年、2010年两年在全国稻区可能暴发褐飞虱对氟虫腈更高水平的抗性。自从2005年10-11月褐飞虱对新烟碱类的吡虫啉产生极高水平抗性（277~811倍）以来，近3年来抗性虽有一定程度下降，但仍处于高水平-极高水平抗性阶段，其中，2008年我国8省13地褐飞虱种群的抗性仍高达210.1~381.7倍。褐飞虱对其他的新烟碱类药剂噻虫嗪、烯啶虫胺和呋虫胺的抗性水平分别为 2.0~15.8、0.7~4.8和0.6~2.8倍。褐飞虱对噻嗪酮为敏感到中等水平抗性（3.0~11.9倍）。近几年来大量、广泛使用氟虫腈防治褐飞虱和稻纵卷叶螟两类迁飞性害虫是褐飞虱对其暴发抗性的重要原因。因此，必须及时制定全国范围防治两类迁飞性害虫的交替轮换用药抗性治理对策方案，才能延缓褐飞虱抗性的再次暴发。

关键词： 褐飞虱; 杀虫剂; 抗药性监测; 抗药性治理

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刘叙杆,赵兴华,王彦华,韦锦捷,沈晋良,孔健,曹明章,周威君,罗才宏 . 褐飞虱对氟虫腈和新烟碱类药剂的抗性动态变化[J]. 中国水稻科学, 2010 , 24(1) : 73 -80 . DOI: DOI: 10.3969/j.issn.1001-7216.2010.01.13

Abstract

The resistances to the phenyl pyrazole (such as fipronil), neonicotinoid and insect growth regulator insecticides (buprofezin) in brown planthopper, Nilaparvata lugens (Stl) were monitored by ricestem dipping method during 2006 to 2008. Results showed that N. lugens sharply developed resistance to fipronil following its immigration route in 2008. The levels of resistance to Fipronil in 10 immigration populations from 6 provinces in the Yangtze River Delta areas and the backmigration populations in Shaoguan and Shenzhen of Guangdong were moderate (resistance ratio 15.0-fold to 32.5-fold) and high (66.9-fold to 73.7-fold), respectively, higher than those of all 6 field populations from 5 provinces in 2006(2.6-fold to 5.8-fold) and 11 populations from 9 provinces in 2007 (3.2-fold to 8.4-fold). The above results suggest that N. lugens would develop a higher level of resistance to fipronil in most ricegrowing areas in China during 2009 to 2010. All populations collected in 2006-2008 showed high or extremely high level of resistance to imidacloprid, of which the resistance levels of 13 populations from 8 provinces in 2008 were 210.1-fold to 381.7-fold, though the resistance level dropped slightly in the following 3 years comparing with the extremely high level in 2005 (277fold to 811fold). For other neonicotinoid insecticides such as hiamethoxam, nitenpyram and dinotefuran, the resistance ratios were in the range of 20fold to 158fold, 07fold to 48fold, and 06fold to 28fold, respectively. All the populations were susceptible or lowlevel resistant to buprofezin (30fold to 119fold). In recent years, widespread and intensive use of fipronil for controlling two classes of migratory rice pest insects (brown planthopper/whitebacked planthopper and rice leaf roller) might be a major reason for fipronil resistance outbreaking in N. lugens. Therefore, to prevent insecticide resistance in N. lugens from outbreaking again, insecticide resistance management strategy including alternating and rotating insecticides without crossresistance need to be established.

Key words： Nilaparvata lugens; insecticides; insecticide resistance monitoring; management

参考文献

[1]程遐年, 吴进才, 马飞. 褐飞虱研究与防治. 北京：中国农业出版社， 2003: 1-37.

[2]Heinrichs E A. Impact of insecticides on the resistance and resurgence of rice planthopper//Denno R F, Perfect T J. Planthopper: Their ecology and management. New York: Chapman and Hall Press, 1994: 571-614.

[3]Matsumura M, Takeuchi H, Satoh M, et al. Speciesspecific insecticide resistance to imidacloprid and fipronil in the rice planthoppers Nilaparvata lugens and Sogatella furcifera in East and Southeast Asia. Pest Manag Sci, 2008, 64(11): 1115-1121.

[4]高希武, 彭丽年, 梁帝允. 对2005年水稻褐飞虱大发生的思考. 植物保护, 2006, 32(2)： 23-25.

[5]程家安, 祝增荣. 2005年长江流域稻区褐飞虱暴发成灾原因分析. 植物保护, 2006, 32(4)： 1-4.

[6]王彦华, 陈进, 沈晋良, 等. 防治褐飞虱的高毒农药替代药剂的室内筛选及交互抗性研究. 中国水稻科学, 2008, 22(5)：519-526.

[7]Wang Y H, Gao C F, Xu Z P, et al. Buprofezin susceptibility survey, resistance selection and preliminary determination of the resistance mechanism in Nilaparvata lugens (Homoptera: Delphacidae). Pest Manag Sci, 2008, 64(10): 1050-1056.

[8]Hirai K. Recent trends of insecticide susceptibility in the brown planthopper Nilaparvata lugens (Stl) (Homoptera: Delphacidae). Appl Entomol Zool, 1993, 28(3): 339-346.

[9]Nagata T. Insecticide resistance and chemical control of the brown planthopper, Nilaparvata lugens (Stl) (Homoptera: Delphacidae). Bull Kyushu Nat Agric Exp Sta, 1982, 22: 49-164.

[10]Wang Y H, Gao C F, Zhu Y C, et al. Imidacloprid susceptibility survey and selection risk assessment in field populations of Nilaparvata lugens (Homoptera: Delphacidae). J Econ Entomol, 2008, 101(2): 515-522.

[11]Grant D B, Chalmers A E, Wolff M A, et al. Fipronil: Action at the GABA receptor//Pesticides and the Future: Minimizing chronic exposure of humans and the environment. Rev Toxicol, 1998, 2: 147-156.

[12]Colliot F, Kukorowski K A, Hawkins D W, et al. Fipronil: A new soil and foliar broad spectrum insecticide//Proceedings of Brighton Crop Protection Conference: Pests and Diseases. Farnham, Surrey, UK: BCPC, 1992: 29-34.

[13]曹明章, 沈晋良, 张金振, 等. 二化螟抗药性监测和对三唑磷抗性的遗传分析. 中国水稻科学, 2004, 18(1)： 73-79.

[14]李淑勇, 刘学, 高聪芬, 等. 防治白背飞虱高毒农药替代药剂的室内筛选及对吡虫啉的抗性风险评估. 中国水稻科学, 2009, 23(1)： 79-84.

[15]何月平, 邵振润, 陈文明, 等. 防治二化螟的高毒农药替代药剂的室内筛选. 中国水稻科学, 2008, 22(3)： 313-320.

[16]高忠文, 陶岭梅, 苏建亚, 等. 防治稻纵卷叶螟高毒农药替代药剂的室内筛选. 中国水稻科学, 2008, 22(6)： 631-636.

[17]Sayyed A H, Wright D J. Fipronil resistance in the diamondback moth (Lepidoptera: Plutelliadae): Inheritance and number of genes involved. J Econ Entomol, 2004, 97(6): 2043-2050.

[18]Kang C Y, Wu G, Miyata T. Synergism of enzyme inhibitors and mechanisms of insecticide resistance in Bimisia tabaci (Gennadius) (Hom, Aleyrodidae). J Appl Entomol, 2006, 130: 377-385.

[19]Ahmad M, Sayyed A H, Saleem M A, et al. Evidence for field evolved resistance to newer insecticides in Spodoptera litura (Lepidoptera: Noctuidae) from Pakistan. Crop Prot, 2008, 27(10): 1367-1372.

[20]He Y P, Gao C F, Cao M Z, et al. Survey of susceptibilities to monosultap, triazophos, fipronil, and abamectin in Chilo suppressalis (Lepidoptera: Crambidae). J Econ Entomol, 2007, 100(6): 1854-1861.

[21]Herron G A, James T M. Monitoring insecticide resistance in Australian Frankiniella occidentalis Pergnade (Thysanoptera: Thripidae) detects fipronil and spinosad resistance. Austral J Entomol, 2005: 44: 299-303.

[22]Prabhaker N, Toscano N C, Perring T M, et al. Resistance monitoring of the sweetpotato whitefly (Homoptera: Aleyrodidae) in the Imperial Valley of California. J Econ Entomol, 1992, 85（4）: 1063-1068.

[23]沈晋良, 吴益东. 棉铃虫抗药性及其治理．北京：中国农业出版社, 1995: 25-88.

[24]庄永林, 沈晋良, 陈峥. 三唑磷对不同翅型稻褐飞虱繁殖力的影响. 南京农业大学学报, 1999, 22（3）： 21-24.

[25]Maienfisch P, Huerlimann H, Rindlisbacher A, et al. The discovery of thiamethoxam: A secondgeneration neonicotinoid. Pest Manag Sci, 2001, 57(2): 165-176.

[26]程霞. 第二代新烟碱类杀虫剂噻虫嗪的开发. 世界农药, 2001, 23（4）： 17-25.

[27]孙建中, 方继朝, 夏礼如, 等. 灭虫精的杀虫活性及田间防治褐飞虱的应用研究. 昆虫学报, 1996, 39（1）： 37-45.

[28]Scott J G. Investigating mechanisms of insecticide resistance: Method, strategies and pitfalls//Roush R T, Tabashnik B E. Pesticide Resistance in Arthropods. New York ﹠ London: Chapaman and Hall, 1990: 39-57.

[29]Cole L M, Roush R T, Casida J E. Drosophila GABAgated chloride channel: Modified [3H] EBOB binding site associated with Ala→Ser or Gly mutants of Rd1 subunit. Life Sci, 1995, 56 (10): 757-765.

[30]Kristensen M, Hansen K K, Jensen K M. Crossresistance between dieldrin and fipronil in German cockroach (Dictyoptera: Blattellidae). J Econ Entomol, 2005, 98(4): 1305-1310.

[31]Liu N, Yue X. Insecticide resistance and crossresistance in the house fly (Diptera: Muscidae). J Econ Entomol, 2000, 93(4): 1269-1275.

[32]Salmeron E, Omoto C. Characterization of deltamethrin and chlorpyirifos resistance in Blattella germanica (L.) (Dictyoptera: Blattellidae) and crossresistance relationships to fipronil. Neotrop Entomol, 2003, 32(1): 177-181.

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