<FONT face="Verdana">水稻幼苗耐缺氧能力的QTL分析</FONT>

王洋,郭媛,洪德林,

doi:10.3969/j.issn.1001-7216.2010.01.04

中国水稻科学 >

2010 , Vol. 24 >Issue 1: 18 - 24

DOI: https://doi.org/10.3969/j.issn.1001-7216.2010.01.04

研究报告

水稻幼苗耐缺氧能力的QTL分析

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1南京农业大学作物遗传与种质创新国家重点实验室，江苏南京 210095； 2 黑龙江大学农业资源与环境学院，黑龙江哈尔滨 150008； *通讯联系人， E-mail: delinhong@njau.edu.cn

收稿日期: 1900-01-01

修回日期: 1900-01-01

网络出版日期: 2010-01-10

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QTL Analysis of the Anoxic Tolerance at the Seedling Stage in Rice

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1State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China; 2 Department of Agricultural Resource and Environment, Heilongjiang University, Harbin 150008, China; *Corresponding author, E-mail: delinhong@njau.edu.cn

Received date: 1900-01-01

Revised date: 1900-01-01

Online published: 2010-01-10

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摘要

利用粳稻品种秀水79与粳稻恢复系C堡及其衍生的247个重组自交系 (RIL)和粳稻Nipponbare与籼稻Kasalath及Nipponbare/Kasalath//Nipponbare 衍生的98个回交重组自交系(BIL)为材料，在缺氧胁迫和正常萌发条件下调查了萌发7 d的幼苗芽鞘长度。以缺氧反应指数为衡量指标，对幼苗耐缺氧能力进行了QTL分析。RIL群体在第2染色体上检测到1个与SSR标记 RM525紧密连锁的QTL qSAT-2-R, 解释表型变异的8.7%；在第7染色体上检测到1个与RM418紧密连锁的QTL qSAT-7-R, 解释表型变异的9.8%；增效等位基因均来自C堡。BIL群体检测到6个QTL,分布在第2、3、5、8、9和12染色体上，分别解释表型变异的16.2%、11.4%、7.3%、5.8%、9.5%和14.0%；其中qSAT-2-B、qSAT-3-B和qSAT-9-B增效等位基因来自Nipponbare。与qSAT-2-B紧密连锁的RFLP标记为C747，C747对应SSR标记RM1367；qSAT-2-R与qSAT-2-B相距7.2 cM。

关键词： 水稻; 幼苗; 耐缺氧能力; 数量性状基因座; 重组自交系; 回交重组自交系

本文引用格式

王洋,郭媛,洪德林, . 水稻幼苗耐缺氧能力的QTL分析[J]. 中国水稻科学, 2010 , 24(1) : 18 -24 . DOI: 10.3969/j.issn.1001-7216.2010.01.04

Abstract

Coleoptile lengths(CL) of seedlings at 7 days after germination under anoxic stress and normal conditions were investigated in two permanently segregated populations and their parents in rice (Oryza sativa L.). By using anoxic response index, a ratio of CL under anoxic stress to CL under normal conditions, as indicator of seedling anoxic tolerance (SAT), QTLs for SAT were detected. Two loci controlling SAT, designated as qSAT-2-R and qSAT-7-R, were detected in the RIL population (247 lines) derived from a cross between Xiushui 79(japonica cultivar) and C Bao(japonica restorer line). qSAT-2-R, explaining 8.7% of phenotypic variation, was tightly linked with the SSR marker RM525. qSAT-7-R, explaining 9.8% of phenotypic variation, was tightly linked with the marker RM418. The positive alleles of the two loci came from C Bao. Six loci controlling SAT, designated as qSAT-2-B, qSAT-3-B, qSAT-5-B, qSAT-8-B, qSAT-9-B and qSAT-12-B, were detected in the BIL population (98 lines) derived from a backcross of Nipponbare (japonica) /Kasalath (indica)//Nipponbare(japonica). The positive alleles of qSAT-2-B, qSAT-3-B and qSAT-9-B, which explained 16.2%, 11.4% and 9.5% of phenotypic variation, respectively, came from Nipponbare. The positive alleles qSAT-5-B, qSAT-8-B and qSAT-12-B, which explained 7.3%, 5.8% and 14.0% of phenotypic variation, respectively, were from Kasalath. The RFLP marker C747, linked tightly with qSAT-2-B, corresponded to the SSR marker RM1367 according to physical map. Genetic distance between qSAT-2-R and qSAT-2-B was 7.2 cM.

Key words： rice (Oryza sativa); seedling; anoxic tolerance; quantitative trait locus; recombinant inbred line; backcross recombinant inbred line

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