中国水稻科学

中国水稻科学

• 研究报告 • 上一篇    下一篇

水稻瘤矮病病毒和矮缩病病毒的核苷酸结合蛋白和核酸结合蛋白及其结合能力分析

梁建设1; 钟伯雄2，*   

  1. 1浙江大学 生物化学有限公司， 浙江 杭州 310029； 2浙江大学 动物科学学院 蚕蜂分子生物学实验室， 浙江 杭州 310029
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2004-05-10 发布日期:2004-05-10

Analysis of Nucleotide Binding Proteins and Nucleic Acid Binding Proteins of Rice Gall Dwarf Virus and Rice Dwarf Virus and Their Binding Ability

LIANG Jian-she1; ZHONG Bo-xiong 2; *   

  1. （1Biochemistry Company Limited; Zhejiang University; Hangzhou 310029; China; 2Laboratory of Molecular Biology of Silkworm and Honeybee; College of Animal Science; ）
  • Received:1900-01-01 Revised:1900-01-01 Online:2004-05-10 Published:2004-05-10

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摘要： 采用α-32P标记的三磷酸核苷酸与病毒粒子共浴的方法及Northwestern分子杂交方法，研究证明了RGDV结构蛋白中，P1蛋白可能是依赖于RNA的RNA聚合酶， P5蛋白可能具有RNA鸟苷酰转移酶功能,P6蛋白可能是核酸结合蛋白，是RGDV复制包装中的关键蛋白。与RGDV结构蛋白具有相同生物功能的RDV的核酸结合蛋白与核酸的结合能力最强，依赖于RNA的RNA聚合酶与核酸的结合能力次之，RNA鸟苷酰转移酶与核酸的结合能力最弱。结果暗示了可以根据与核酸的结合能力，辅助判断RDV或RGDV这3个结构蛋白的生物功能。

关键词: 水稻瘤矮病病毒, 水稻矮缩病病毒, 依赖于RNA的RNA聚合酶, RNA鸟苷酰转移酶, 核酸结合蛋白

Abstract: By incubation of virion with 〔α- 32P〕GTP, 〔α- 32P〕ATP, 〔α- 32P〕CTP and 〔α- 32P〕UTP and Northwestern blotting, the function of structural proteins from rice gall dwarf virus (RGDV) was determined. In the structural proteins, the P1 was the putative RNA-dependent RNA polymerase, which might play an important role in the synthesis of RGDV genomic dsRNAs;The P5 bound with GTP and UTP was a NTP binding protein with RNA guanylyltransferase activity that synthesized a cap structure (mGpppAmpGp) for mRNA of RGDV; The P6 was RNA binding protein and might play an important role in RGDV particle package, as a “key protein” for the assembly of genomic RGDV dsRNAs into the viral particle. The structural protein with the strongest binding ability to nucleic acid was the nucleic acid binding protein of the protein P7 in rice dwarf virus (RDV) which has the similar biological function with the structural protein of the protein P6 in RGDV; the second one was RNA-dependent RNA polymerase; and the lowest one was RNA guanylyltransferase. It indicated that the biological function of the three structural proteins of RDV or RGDV could be judged auxiliary by their binding ability to the nucleic acid.

Key words: rice gall dwarf virus, rice dwarf virus, RNA-dependent RNA polymerase, RNA guanyltransferase, RNA binding protein