中国水稻科学

中国水稻科学

• 实验技术 • 上一篇    下一篇

水稻细菌性条斑病种子带菌检测技术研究：I． 免疫放射分析法

谢关林1；孙漱沅1；王公金2；朱献玳2；陈军昂3；叶彦弧3；冯忠民4；梁梅新4   

  1. 1 浙江省农业科学院，杭州31002l；2 江苏省农业科学院，南京210014；3 浙江省植物检疫站，杭州310OO4；4 浙江省新昌县农业局，浙江新昌312500
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1990-09-17 发布日期:1990-09-17

Studies on Rice Seed Inspection of Xanthomonas campestris pv. oryzicola:Ⅰ. Immunoradiometric Assay

Xie Guanlin; Sun Shuyuan; Wang Gongjin; Zhu Xiandai; Chen Junang; Ye Yanhu; Feng Zhongmin; Liang Meixin   

  • Received:1900-01-01 Revised:1900-01-01 Online:1990-09-17 Published:1990-09-17

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摘要： 免疫放射分析法（IRMA）集放射性同位素的灵敏性与抗原-抗体反应的特异性为一体。该方法应用于水稻细菌性条斑病种子带菌检测，经三年试验表明这一方法具有灵敏度高(最小检测浓度为10^3细菌／ml)。特异性强，稳定性好。快速(4～5小时内可得出结果)。通过402份样品的检测，经与常规的浓缩接种法比较，表明该方法应用于带菌稻种的检测前景广阔。

关键词: 水稻细菌性条斑病菌, 种子检测, 免疫放射分析法

Abstract: Immunoradiometric assay (IRMA) is a technique of superior trace analysis which integrated the specificity of serology and sensitivity of radioisotope. With the method in rice seed inspection of X. c. pv. oryzicola for 3 years. 402 seed samples were tested. The results showed that equal or above 1000 bacterial cells per ml of X.c. pv. oryicola in the seeds could be detected with the IRMA. The antiserum and labelled protein A was stable and only slight cross-reaction with X. c. pv. oryzae. The process of seed inspection for a batch of seeds could be finished in 4-5 hours if the antiserum and labelled protein A was prepared Compared with the concentration inoculation of common methods, the IRMA was more effective and reliable.

Key words: Xanthomonas campestris pv. oryzicola, Seed inspection, Immunoradiometric assay