杂交粳稻亲本米质性状配合力的分子标记区段检测

doi:10．3969/j．issn．1001G7216．2015．04．006

摘要/Abstract

摘要：

选用152个SSR引物扩增11个粳稻细胞质雄性不育系和9个粳稻恢复系的分子标记基因型,并按NCⅡ遗传设计配制99个F₁组合,检测20个亲本中有关糙米率、精米率、整精米率、垩白粒率、垩白度、碱消值、胶稠度和直链淀粉含量8个米质性状的一般配合力的分子标记区段。结果发现27个分子标记区段与亲本8个米质性状一般配合力显著相关,共包含49个分子标记区段基因型。其中,31个SSR分子标记区段基因型与亲本8个米质性状优异一般配合力显著相关,涉及14条染色体臂;18个SSR分子标记区段与亲本8个米质性状不良一般配合力显著相关。49个分子标记区段基因型中,有2个分子标记区段基因型与亲本4个米质性状一般配合力共相关;2个分子标记区段基因型分别与亲本3个米质性状一般配合力共相关,8个分子标记区段基因型分别与亲本2个米质性状一般配合力共相关。第7染色体长臂上的分子标记区段基因型RM542-80/105与碱消值相关,增效37.0%;第1染色体短臂上的分子标记区段基因型RM246-95/105与直链淀粉含量有关,减效23.8%;第8染色体长臂上的分子标记区段基因型RM3754-80/90可使F₁糙米率、精米率、整精米率和碱消值分别增加1.1%、2.0%、2.4%和43.6%。这些分子标记区段基因型可直接用于改良杂交粳稻亲本米质性状一般配合力。

关键词: 杂交粳稻, 米质, SSR分子标记, 配合力, 分子标记区段基因型

Abstract:

In order to improve rice quality combining ability of parental lines more efficiently in hybrid japonica rice(Oryza sativa L.), SSR molecular marker fragment genotypes of elite combining ability for eight quality traits were identified by analyzing the data of combining ability and SSR markers in eleven cytoplasmic male sterile(CMS) lines and nine restorer lines. The eight quality traits investigated were brown rice rate (BRR), milled rice rate (MRR), head rice rate (HRR), percentage of chalky grain, chalkiness degree, alkali spreading value (ASV), gel consistency, and amylose contents. The 11 CMS lines and 9 restorer lines were genotyped using 152 pairs of SSR primers. Combining ability analysis was conducted using the data of 99 F₁s made with NCII genetic design (11 CMS lines×9 restorer lines). A total of 49 SSR molecular marker fragment genotypes involving 27 molecular marker fragments were significantly associated with general combining ability of the 8 quality traits. 31 SSR molecular marker fragment genotypes on 14 chromosome arms were associated with elite combining ability for quality traits of parents; the remaining 18 molecular marker fragment genotypes were associated with unfavorable combining ability of quality traits of parents. Among the 49 molecular marker fragment genotypes, two were co-associated with four quality traits of parents, and two were each co-associated with three quality traits of parents, and eight were each co-associated with two quality traits of parents. The molecular marker fragment genotype RM542-80/105 on the long arm of chromosome 7 increased alkali spreading value in F₁ by 37.0%, RM246-95/105 on the short arm of chromosome 1 decreased amylose contents in F₁ by 23.8%, RM3754-80/90 on the long arm of the chromosome 8 increased the traits of BRR, MRR, HRR and ASV in F₁ by 1.1%, 2.0%, 2.4% and 43.6%, respectively. The molecular marker fragment genotypes could be directly used for improving general combining ability of grain quality traits of parents in japonica rice.

Key words: hybrid japonica rice, rice quality, SSR molecular marker, combining ability, molecular marker fragment genotype

刘洋, 刘二宝, 曾思远, 普伟, 刘强明, 梁利君, 刘佩睿, 张琪梦, 洪德林. 杂交粳稻亲本米质性状配合力的分子标记区段检测[J]. 中国水稻科学, 2015, 29(4): 373-381.

Yang LIU, Er-bao LIU, Si-yuan ZENG, Wei PU, Qiang-ming LIU, Li-jun LIANG, Pei-rui LIU, Qi-meng ZHANG, De-lin HONG. Identification of Molecular Marker Fragments Associated with Combining Ability for Quality Traits in Parents of Hybrid japonica Rice (Oryza sativa L.)[J]. Chinese Journal OF Rice Science, 2015, 29(4): 373-381.

图/表 4

参考文献 20

[1]	李建红, 洪德林. 新选粳稻BT型不育系主要农艺及品质性状的配合力分析. 南京农业大学学报, 2004, 27(4): 11-16.
[2]	李建红, 洪德林. 杂交粳稻主要农艺性状和同质恢复系农艺及品质性状配合力研究. 作物学报, 2005, 31(7): 851-857.
[3]	金伟栋, 张旺, 洪德林. 苏南地区晚熟粳稻杂种优势及其亲本配合力分析. 作物学报, 2005, 31(11): 1478-1484.
[4]	刘小川, 王渭霞, 陈深广, 等. 杂交稻米质性状的亲本配合力分子标记鉴定. 中国水稻科学, 2005, 19(1): 25-28.
[5]	Liu X C, Wu J L.SSR heterogenic patterns of parents for marking and predicting heterosis in rice breeding.Mol Breeding, 1998(4): 263-268.
[6]	Liu X C, Ishiki K, Wang W X.Identification of favorable AFLP markers to heterosis in hybrid rice.Breeding Sci, 2002, 52: 201-206.
[7]	Liu X C, Chen S C, Chen J S, et al.Improvement of combining ability for restorer lines with identified SSR marker in hybrid rice breeding.Breeding Sci, 2004, 54: 341-346.
[8]	黄殿成. 长江中下游杂交粳稻亲本产量和品质性状优异配合力标记基因型筛选与配合力改良研究[学位论文]. 南京: 南京农业大学, 2012: 69-103.
[9]	黄殿成, 梁奎, 孙程, 等. 杂交粳稻亲本米质性状优异配合力的标记基因型鉴定. 作物学报, 2011, 37(3): 405-414.
[10]	梁奎, 黄殿成, 赵凯铭, 等. 杂交粳稻亲本产量性状优异配合力的标记基因型筛选. 作物学报, 2010, 36(8): 1270-1279.
[11]	黄殿成, 阮方松, 刘健, 等. 杂交粳稻亲本穗长和枝梗数性状优异配合力的标记基因型筛选. 南京农业大学学报, 2011, 34(2): 1-7.
[12]	刘二宝, 刘洋, 刘晓丽, 等. 杂交粳稻亲本产量性状优异配合力的标记基因型检测. 中国水稻科学, 2013, 27(5): 473-481.
[13]	韩龙植, 魏兴华. 水稻种质资源描述规范和数据标准. 北京: 中国农业出版社, 2006: 89-90.
[14]	洪德林. 作物育种学实验技术. 2版. 北京: 科学出版社, 2010: 12-21.
[15]	朱小杨, 沈圣泉, 陈文岳, 等. 酶标仪在稻米表观直链淀粉含量测定及突变体筛选中的应用. 核农学报, 2004, 18(3): 183-185.
[16]	程保山, 万志兵, 洪德林. 35个粳稻品种SSR指纹图谱的构建及遗传相似性分析. 南京农业大学学报, 2007, 30(3): 1-8.
[17]	时宽玉, 洪德林. 6个水稻杂交组合与其亲本的SSR标记多态性及其应用. 南京农业大学学报, 2005, 28(4): 1-5.
[18]	莫惠栋. p×q交配模式的配合力分析. 江苏农学院学报, 1982, 3: 51-57.
[19]	莫惠栋. p×q交配模式的配合力分析(续). 江苏农学院学报, 1982, 3(4): 53-57.
[20]	徐辰武, 张爱红, 朱庆森, 等. 籼粳杂交稻米品质性状的遗传分析. 作物学报, 1996, 22(5): 530-534.

亲本 Parent	糙米率 BRR	精米率 MRR	整精米率 HRR	垩白粒率 PCG	垩白度 CD	胶稠度 GC
95122A	-0.35	-0.25	-0.17	-16.86^**	-7.48^**	2.52
90167A	0.41^*	0.26	0.38	11.97^**	4.20^**	2.04
863A	-0.54^**	-0.44	-0.23	-6.41^**	-3.05^**	-0.87
1004S	0.57^**	1.15^**	1.29^**	5.64^**	1.92^*	-4.93
A171	1.44^**	1.72^**	1.63^**	10.53^**	3.85^**	-2.35
A150	-0.96^**	-1.81^**	-2.43^**	6.92^**	3.72^**	-1.37
爱之乡A Aizhixiang A	-0.52^**	0.01	0.32	-13.80^**	-4.57^**	-0.21
18A	0.37	-0.37	-0.68^*	0.92	-0.30	11.02^*
浙04A Zhe 04A	0.29	0.68^**	1.01^**	-11.25^**	-2.68^**	-5.21
春江19A Chunjiang 19A	-0.43^*	-0.15	-0.15	2.75	1.62^*	-5.82
春江18A Chunjiang 18A	-0.28	-0.81^**	-0.98^**	9.59^**	2.77^**	5.18
R4179	0.01	0.02	-0.02	-13.05^**	-3.15^**	-0.78
LC64	0.20	0.20	0.24	-6.59^**	-1.25	1.56
LC109	0.21	0.07	0.14	-6.95^**	-3.79^**	-5.53
盐恢R50 Yanhui R50	-0.52^**	-0.50^*	-0.41	-2.55	-0.27	-0.35
盐恢R8 Yanhui R8	0.29	-0.26	-0.17	4.27^*	-1.73^*	0.61
LR5	-0.79^**	-0.34	-0.51	14.55^**	4.56^**	1.40
LR27	0.58^**	0.99^**	1.10^**	-1.77	1.37	1.54
申恢254 Shenhui 254	0.84^**	0.50^*	0.49	5.82^**	1.82^*	5.54
C4115	-0.82^**	-0.66^**	-0.85^**	6.27^**	2.44^**	-3.98
亲本 Parent	碱消值 ASV	直链淀粉含量 AC	GCA总秩次 Total rank of GCA	排序 Sequence	特殊配合力方差总秩次 Total rank of V_SCA	排序 Sequence
95122A	0.16	-2.49	35	2	52	6
90167A	-0.64^**	-4.41^*	47	6	37	2
863A	0.96^**	-0.73	49	7	46	4
1004S	0.84^**	-3.82	35	2	48	5
A171	1.87^**	-1.52	37	3	56	8
A150	-1.26^**	2.95	74	11	57	9
爱之乡A AizhixiangA	-0.15	4.65^*	44	5	53	7
18A	-0.63^**	0.98	49	7	52	6
浙04A Zhe 04 A	1.21^**	-3.38	33	1	41	3
春江19A Chunjiang19A	-0.40^**	0.36	57	9	56	8
春江18A Chunjiang18A	-1.94^**	7.41^**	68	10	30	1
R4179	0.85^**	0.40	32	4	30	2
LC64	-0.99^**	-1.85	30	3	52	8
LC109	-1.26^**	1.17	41	6	51	7
盐恢R50 Yanhui R50	0.24^**	-1.91	42	7	40	3
盐恢R8 Yanhui R8	0.34^**	0.88	38	5	48	6
LR5	0.10	1.64	61	9	42	5
LR27	0.34^**	0.98	26	1	26	1
申恢254 Shenhui 254	0.18^*	-1.65	29	2	41	4
C4115	0.20^*	0.34	60	8	30	2

亲本 Parent	糙米率 BRR	精米率 MRR	整精米率 HRR	垩白粒率 PCG	垩白度 CD	胶稠度 GC
95122A	-0.35	-0.25	-0.17	-16.86^**	-7.48^**	2.52
90167A	0.41^*	0.26	0.38	11.97^**	4.20^**	2.04
863A	-0.54^**	-0.44	-0.23	-6.41^**	-3.05^**	-0.87
1004S	0.57^**	1.15^**	1.29^**	5.64^**	1.92^*	-4.93
A171	1.44^**	1.72^**	1.63^**	10.53^**	3.85^**	-2.35
A150	-0.96^**	-1.81^**	-2.43^**	6.92^**	3.72^**	-1.37
爱之乡A Aizhixiang A	-0.52^**	0.01	0.32	-13.80^**	-4.57^**	-0.21
18A	0.37	-0.37	-0.68^*	0.92	-0.30	11.02^*
浙04A Zhe 04A	0.29	0.68^**	1.01^**	-11.25^**	-2.68^**	-5.21
春江19A Chunjiang 19A	-0.43^*	-0.15	-0.15	2.75	1.62^*	-5.82
春江18A Chunjiang 18A	-0.28	-0.81^**	-0.98^**	9.59^**	2.77^**	5.18
R4179	0.01	0.02	-0.02	-13.05^**	-3.15^**	-0.78
LC64	0.20	0.20	0.24	-6.59^**	-1.25	1.56
LC109	0.21	0.07	0.14	-6.95^**	-3.79^**	-5.53
盐恢R50 Yanhui R50	-0.52^**	-0.50^*	-0.41	-2.55	-0.27	-0.35
盐恢R8 Yanhui R8	0.29	-0.26	-0.17	4.27^*	-1.73^*	0.61
LR5	-0.79^**	-0.34	-0.51	14.55^**	4.56^**	1.40
LR27	0.58^**	0.99^**	1.10^**	-1.77	1.37	1.54
申恢254 Shenhui 254	0.84^**	0.50^*	0.49	5.82^**	1.82^*	5.54
C4115	-0.82^**	-0.66^**	-0.85^**	6.27^**	2.44^**	-3.98
亲本 Parent	碱消值 ASV	直链淀粉含量 AC	GCA总秩次 Total rank of GCA	排序 Sequence	特殊配合力方差总秩次 Total rank of V_SCA	排序 Sequence
95122A	0.16	-2.49	35	2	52	6
90167A	-0.64^**	-4.41^*	47	6	37	2
863A	0.96^**	-0.73	49	7	46	4
1004S	0.84^**	-3.82	35	2	48	5
A171	1.87^**	-1.52	37	3	56	8
A150	-1.26^**	2.95	74	11	57	9
爱之乡A AizhixiangA	-0.15	4.65^*	44	5	53	7
18A	-0.63^**	0.98	49	7	52	6
浙04A Zhe 04 A	1.21^**	-3.38	33	1	41	3
春江19A Chunjiang19A	-0.40^**	0.36	57	9	56	8
春江18A Chunjiang18A	-1.94^**	7.41^**	68	10	30	1
R4179	0.85^**	0.40	32	4	30	2
LC64	-0.99^**	-1.85	30	3	52	8
LC109	-1.26^**	1.17	41	6	51	7
盐恢R50 Yanhui R50	0.24^**	-1.91	42	7	40	3
盐恢R8 Yanhui R8	0.34^**	0.88	38	5	48	6
LR5	0.10	1.64	61	9	42	5
LR27	0.34^**	0.98	26	1	26	1
申恢254 Shenhui 254	0.18^*	-1.65	29	2	41	4
C4115	0.20^*	0.34	60	8	30	2

性状 Trait	分子标记区段基因型 Molecular marker fragment genotype	纯合组 Homozygous group			杂合组 Heterozygous group	t 值 t-value
性状 Trait	分子标记区段基因型 Molecular marker fragment genotype	F₁组合数 Number of F₁ cross		各 F₁组合平均数 Average of F₁’s		F₁组合数 Number of F₁ cross	各 F₁组合平均数 Average of F₁’s
糙米率 BRR	RM587-165/170(6S)	72	83.44	27	84.63	1.4	4.37
	RM345-160/170(6L)	78	83.60	21	84.37	0.9	2.47
	RM3754-80/90(8L)	81	83.59	18	84.53	1.1	2.85
	RM5348-190/195(10L)	63	83.52	36	84.18	0.8	2.45
精米率 MRR	RM208-190/195(2L)	64	73.51	35	74.42	1.2	2.64
	RM3586-180/190(3L)	67	74.11	32	73.26	-1.1	-2.38
	RM148-145/150(3L)	54	74.19	45	73.41	-1.0	-2.35
	RM3586-180/185(3L)	51	73.33	48	74.37	1.4	3.23
	RM587-165/170(6S)	72	73.35	27	75.14	2.5	5.34
	RM3754-80/90(8L)	81	73.56	18	75.05	2.0	3.59
	RM228-105/110(10L)	72	73.57	27	74.55	1.3	2.68
整精米率HRR	RM148-145/150(3L)	54	73.52	45	72.65	-1.2	-2.29
	RM3586-180/185(3L)	51	72.52	48	73.78	1.7	3.42
	RM3586-180/190(3L)	67	73.44	32	72.47	-1.3	-2.40
	RM587-165/170(6S)	72	72.62	27	74.48	2.6	4.72
	RM6670-110/130(8S)	59	72.72	40	73.72	1.4	2.61
	RM3754-80/90(8L)	81	72.81	18	74.56	2.4	3.71
	RM228-105/110(10L)	72	72.82	27	73.94	1.5	2.65
垩白粒率PCG	RM471-105/110(4L)	40	39.11	59	29.92	-23.5	-3.02
	RM478-190/205(7L)	63	30.54	36	39.06	27.9	2.72
	RM215-170/175(9L)	75	36.58	24	24.44	-33.2	-3.54
	RM167-160/180(11S)	74	30.19	25	43.84	45.2	4.11
	RM17-170/180(12L)	74	37.28	25	22.86	-38.7	-4.39
垩白度CD	RM5699-170/175(2S)	83	13.33	16	6.48	-51.4	-4.06
	RM208-190/195(2L)	64	10.90	35	14.65	34.4	2.77
	RM207-130/140(2L)	81	11.44	18	15.75	37.6	2.55
	RM336-180/185(7L)	78	11.44	21	15.15	32.5	2.32
	RM167-160/180(11S)	74	10.89	25	16.18	48.5	3.64
	RM17-170/180(12L)	74	13.53	25	8.36	-38.2	-3.56
胶稠度 GC	RM227-100/105(3L)	67	101.10	31	87.97	-13.0	-2.50
	RM317-155/160(4L)	73	93.86	25	105.96	12.9	2.14
	RM542-80/95(7L)	83	94.74	15	109.17	15.2	2.11
	RM467-185/190(10L)	84	94.85	14	109.54	15.5	2.08
	RM467-180/190(10L)	84	99.02	14	84.50	-14.7	-2.06
碱消值ASV	RM341-140/175(2L)	50	3.82	49	4.96	29.7	3.64
	RM208-190/195(2L)	64	4.01	35	5.08	26.8	3.25
	RM148-145/150(3L)	54	4.74	45	3.95	-16.7	-2.44
	RM85-65/70(3L)	62	4.01	37	5.02	25.3	3.10
	RM471-105/110(4L)	40	3.78	59	4.79	26.7	3.13
	RM317-155/160(4L)	74	4.63	25	3.67	-20.7	-2.58
	RM402-125/130(6L)	47	4.93	52	3.89	-21.0	-3.27
	RM542-80/105(7L)	74	4.01	25	5.49	37.0	4.22
	RM3754-80/90(8L)	81	4.06	18	5.83	43.6	4.52
	RM3700-140/150(9L)	85	4.57	14	3.25	-28.9	-2.89
	RM4B-150/170(11S)	72	4.01	27	5.39	34.3	3.98
直链淀粉含量AC	RM246-95/105(1S)	79	18.39	20	14.01	-23.8	-2.68
	RM208-190/195(2L)	64	18.84	35	15.05	-20.1	-2.78
	RM402-125/130(6L)	47	15.87	52	18.97	19.5	2.34
	RM478-195/205(7L)	75	16.61	24	20.27	22.0	2.38

性状 Trait	分子标记区段基因型 Molecular marker fragment genotype	纯合组 Homozygous group			杂合组 Heterozygous group	t 值 t-value
性状 Trait	分子标记区段基因型 Molecular marker fragment genotype	F₁组合数 Number of F₁ cross		各 F₁组合平均数 Average of F₁’s		F₁组合数 Number of F₁ cross	各 F₁组合平均数 Average of F₁’s
糙米率 BRR	RM587-165/170(6S)	72	83.44	27	84.63	1.4	4.37
	RM345-160/170(6L)	78	83.60	21	84.37	0.9	2.47
	RM3754-80/90(8L)	81	83.59	18	84.53	1.1	2.85
	RM5348-190/195(10L)	63	83.52	36	84.18	0.8	2.45
精米率 MRR	RM208-190/195(2L)	64	73.51	35	74.42	1.2	2.64
	RM3586-180/190(3L)	67	74.11	32	73.26	-1.1	-2.38
	RM148-145/150(3L)	54	74.19	45	73.41	-1.0	-2.35
	RM3586-180/185(3L)	51	73.33	48	74.37	1.4	3.23
	RM587-165/170(6S)	72	73.35	27	75.14	2.5	5.34
	RM3754-80/90(8L)	81	73.56	18	75.05	2.0	3.59
	RM228-105/110(10L)	72	73.57	27	74.55	1.3	2.68
整精米率HRR	RM148-145/150(3L)	54	73.52	45	72.65	-1.2	-2.29
	RM3586-180/185(3L)	51	72.52	48	73.78	1.7	3.42
	RM3586-180/190(3L)	67	73.44	32	72.47	-1.3	-2.40
	RM587-165/170(6S)	72	72.62	27	74.48	2.6	4.72
	RM6670-110/130(8S)	59	72.72	40	73.72	1.4	2.61
	RM3754-80/90(8L)	81	72.81	18	74.56	2.4	3.71
	RM228-105/110(10L)	72	72.82	27	73.94	1.5	2.65
垩白粒率PCG	RM471-105/110(4L)	40	39.11	59	29.92	-23.5	-3.02
	RM478-190/205(7L)	63	30.54	36	39.06	27.9	2.72
	RM215-170/175(9L)	75	36.58	24	24.44	-33.2	-3.54
	RM167-160/180(11S)	74	30.19	25	43.84	45.2	4.11
	RM17-170/180(12L)	74	37.28	25	22.86	-38.7	-4.39
垩白度CD	RM5699-170/175(2S)	83	13.33	16	6.48	-51.4	-4.06
	RM208-190/195(2L)	64	10.90	35	14.65	34.4	2.77
	RM207-130/140(2L)	81	11.44	18	15.75	37.6	2.55
	RM336-180/185(7L)	78	11.44	21	15.15	32.5	2.32
	RM167-160/180(11S)	74	10.89	25	16.18	48.5	3.64
	RM17-170/180(12L)	74	13.53	25	8.36	-38.2	-3.56
胶稠度 GC	RM227-100/105(3L)	67	101.10	31	87.97	-13.0	-2.50
	RM317-155/160(4L)	73	93.86	25	105.96	12.9	2.14
	RM542-80/95(7L)	83	94.74	15	109.17	15.2	2.11
	RM467-185/190(10L)	84	94.85	14	109.54	15.5	2.08
	RM467-180/190(10L)	84	99.02	14	84.50	-14.7	-2.06
碱消值ASV	RM341-140/175(2L)	50	3.82	49	4.96	29.7	3.64
	RM208-190/195(2L)	64	4.01	35	5.08	26.8	3.25
	RM148-145/150(3L)	54	4.74	45	3.95	-16.7	-2.44
	RM85-65/70(3L)	62	4.01	37	5.02	25.3	3.10
	RM471-105/110(4L)	40	3.78	59	4.79	26.7	3.13
	RM317-155/160(4L)	74	4.63	25	3.67	-20.7	-2.58
	RM402-125/130(6L)	47	4.93	52	3.89	-21.0	-3.27
	RM542-80/105(7L)	74	4.01	25	5.49	37.0	4.22
	RM3754-80/90(8L)	81	4.06	18	5.83	43.6	4.52
	RM3700-140/150(9L)	85	4.57	14	3.25	-28.9	-2.89
	RM4B-150/170(11S)	72	4.01	27	5.39	34.3	3.98
直链淀粉含量AC	RM246-95/105(1S)	79	18.39	20	14.01	-23.8	-2.68
	RM208-190/195(2L)	64	18.84	35	15.05	-20.1	-2.78
	RM402-125/130(6L)	47	15.87	52	18.97	19.5	2.34
	RM478-195/205(7L)	75	16.61	24	20.27	22.0	2.38

相关性状个数 No. of correlated traits	分子标记区段基因型 Molecular marker fragment genotype	糙米率 BRR	精米率 MRR	整精米率 HRR	垩白粒率 PCG	垩白度 CD	胶稠度 GC	碱消值 ASV	直链淀粉含量 AC
4	RM208-190/195(2L)		1.2			34.4		26.8	-20.1
	RM3754-80/90(8L)	1.1	2.0	2.4				43.6
3	RM148-145/150(3L)		-1.0	-1.2				-16.7
	RM587-165/170(6S)	1.4	2.5	2.6
2	RM3586-180/185(3L)		1.4	1.7
	RM3586-180/190(3L)		-1.1	-1.3
	RM317-155/160(4L)						12.9	-20.7
	RM471-105/110(4L)				-23.5			26.7
	RM402-125/130(6S)							-21.0	19.5
	RM228-105/110(10L)		1.3	1.5
	RM167-160/180(11S)				45.2	48.5
	RM17-170/180(12L)				-4.4	-3.6
1	RM246-95/105(1S)								-23.8
	RM5699-170/175(2S)					-51.4
	RM207-130/140(2L)					37.6
	RM341-140/175(2L)							29.7
	RM227-100/105(3L)						-13.0
	RM85-65/70(3L)							25.3
	RM345-160/170(6L)	0.9
	RM542-80/95(7L)						15.2
	RM542-80/105(7L)							37.0
	RM478-190/205(7L)				27.9
	RM478-195/205(7L)								22.0
	RM336-180/185(7L)					32.5
	RM6670-110/130(8S)			1.4
	RM3700-140/150(9L)							-28.9
	RM215-170/175(9L)				-33.2
	RM5348-190/195(10S)	0.8
	RM467-185/190(10L)						15.5
	RM467-180/190(10L)						-14.7
	RM4B-150/170(11S)							34.3