利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能

doi:10.3969/j.issn.10017216.2013.01.002

中国水稻科学 ›› 2013, Vol. 27 ›› Issue (1): 9-16.DOI: 10.3969/j.issn.10017216.2013.01.002

利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能

玉晓红¹ ,晁丽¹,陈昌冬¹ ,刘永胜^1，2 ,牛向丽^{1, 2, *}

¹重庆大学生命科学学院，重庆 400044; ²合肥工业大学生物与食品工程学院，安徽合肥 230009；

收稿日期:2012-03-21 修回日期:2012-05-02 出版日期:2013-01-10 发布日期:2013-01-10
通讯作者: 牛向丽1, 2, *
基金资助:
国家自然科学基金资助项目(30971752, 30970260)；重庆市科技攻关计划资助项目(2010AA1019)。

Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference

YU Xiaohong ¹, CHAO Li ¹, CHEN Changdong ¹, LIU Yongsheng ^{1, 2}, NIU Xiangli ^{1, 2, *}

¹ College of Life Sciences, Chongqing University, Chongqing 400044, China; ² School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, China;

Received:2012-03-21 Revised:2012-05-02 Online:2013-01-10 Published:2013-01-10
Contact: NIU Xiangli 1, 2, *

摘要/Abstract

摘要： 通过RNA干涉对一个水稻锌指蛋白基因OsZRL的功能进行分析。半定量RTPCR、定量PCR分析结果显示，转基因植株OsZRL基因表达水平显著下调。与野生型相比，OsZRL表达水平下调的转基因株系叶片变大，根系、茎秆更为发达，表明OsZRL的下调对水稻植株的生长有促进作用。OsZRL基因的表达模式和转基因幼苗表型显示OsZRL参与赤霉素、脱落酸信号途径。因而推测锌指蛋白OsZRL是受赤霉素、脱落酸调节的水稻生长发育负调控因子。

关键词: 水稻锌指蛋白, RNA干涉, 负调控, 赤霉素, 脱落酸

Abstract: The function of a rice zincfinger protein gene (OsZRL) was investigated by RNA interference. The results of semiquantitative RTPCR and quantitative PCR analysis indicated that OsZRL expression levels were significantly downregulated in the transgenic plants. The OsZRLRNAi transgenic lines were characterized by bigger leaves, stronger roots and stems compared to wild type plants, indicating that the suppression of OsZRL had a positive effect on rice growth. The expression pattern and transgenic seedlings phenotypes revealed that OsZRL was involved in gibberellin or abscisic acid signaling pathway. It suggests that zincfinger protein OsZRL might perform its function of negetive regulator in rice growth through the regulation of gibberellin and abscisic acid.

Key words: rice zincfinger protein, RNA interference, negative regulation, gibberellin, abscisic acid

中图分类号:

玉晓红1 ,晁丽1,陈昌冬1 ,刘永胜1，2 ,牛向丽1, 2, *. 利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能[J]. 中国水稻科学, 2013, 27(1): 9-16.

YU Xiaohong1, CHAO Li1, CHEN Changdong1, LIU Yongsheng1, 2, NIU Xiangli 1, 2, *. Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference[J]. Chinese Journal of Rice Science, 2013, 27(1): 9-16.

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利用RNA干涉研究水稻锌指蛋白基因OsZRL的功能

Function Analysis of a Rice Zinc Finger Protein Gene OsZRL by RNA Interference

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