中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (1): 5-8.DOI: 10.3969/j.issn.10017216.2012.01.002

• 研究报告 • 上一篇    下一篇

OsBTF3过量表达和RNAi转基因水稻抗盐和抗低温胁迫鉴定

李广旭1, 2陈华民1吴茂森1何晨阳1， *   

  1. 1中国农业科学院 植物保护研究所/植物病虫害生物学国家重点实验室，北京100193；2辽宁省农业科学院 果树研究所， 辽宁 熊岳 115009;
  • 收稿日期:2011-08-31 修回日期:2011-10-20 出版日期:2012-01-10 发布日期:2012-01-10
  • 通讯作者: 何晨阳1， *
  • 基金资助:

    国家转基因生物新品种培育重大专项资助项目 (2009ZX08009044B, 2011ZX08001002)。

Resistance to High Salt and  Cold Stress of Transgenic Rice Seedlings with Overexpressed and RNAisilenced OsBTF3

LI Guangxu1, 2, CHEN Huamin1, WU Maosen1, HE  Chenyang1,*   

  1. 1 State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193,  China;  2 Institute of Pomology, Liaoning Academy of Agricultural Sciences, Xiongyue 115009, China;
  • Received:2011-08-31 Revised:2011-10-20 Online:2012-01-10 Published:2012-01-10
  • Contact: HE Chenyang1,*

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被引次数

11

摘要： 为评估水稻OsBTF3基因在水稻对非生物逆境胁迫应答中的功能，利用RTQPCR技术，对经高盐(200 mmol/L NaCl溶液)和低温(4℃)胁迫处理后水稻的OsBTF3基因表达动态进行了测定，并对前期获得的OsBTF3过量表达和RNAi转基因水稻株系进行了抗高盐和抗低温胁迫的鉴定。结果表明，高盐胁迫处理显著抑制了OsBTF3基因的表达，低温胁迫对其表达影响有一定的变化；转基因T2代过量表达株系对高盐和低温胁迫抗性显著增强，而RNAi转基因株系抗性减弱，说明OsBTF3可能在水稻对高盐和低温胁迫反应中起着重要的调控作用。

关键词: OsBTF3, 基因表达； 抗逆境胁迫

Abstract: To demonstrate the biological function of OsBTF3 gene in rice resistance  or tolerance to salt and cold stress, the gene transcription of OsBTF3 responsive to salt and cold treatments was quantified via realtime PCR assay, and resistance to the stress of transgenic rice seedlings with overexpressed and RNAisilenced OsBTF3 was analyzed. The  results  showed that gene expression of OsBTF3 was significantly decreased in rice plants under salt stress, while lightly reduced under  cold stress. The resistance to salt and cold stress was  enhanced highly  in the seedlings of transgenic lines with overexpressed OsBTF3, while   decreased   in the RNAisilenced OsBTF3 lines. The results revealed that  OsBTF3 might function as a key regulator for abiotic stress resistance in rice. 

Key words: OsBTF3, gene expression, abiotic stress

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