关键词: 转基因水稻, 位置效应, 富赖氨酸蛋白基因, 热不对称交错聚合酶链式反应, 赖氨酸含量, 蛋白质含量

Abstract: The lysine-rich protein gene sb401 had been successfully introduced into a japonica rice variety Nipponbare by Agrobacterium-mediated transformation.Out of ten independent transformed lines,nine homozygous T4 generation transgenic lines were obtained through consecutive selfing.Southern blotting analysis of T4 lines showed that the integration sites of the foreign gene were random with low copy numbers(1 to 3).TAIL-PCR analysis showed that eight T-DNA flanking sequences were located on seven chromosomes.Detection of protein and amino acids contents of T4 lines showed thatsb401 gene contributed,to some extent,to the increase of lysine and other amino acids contents in transgenic lines.The analysis combining blotting and T-DNA position showed that the expression difference was not obvious when there were low copies in transgenic rice lines.

Key words: transgenic rice, position effect, lysine-rich protein gene, thermal asymmetric interlace polymerase chain reaction, lysine content, protein content