中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    

水稻结实期间叶片衰老与膜脂过氧化的关系

段 俊;梁承邺;张明永;黄毓文;刘鸿先   

  1. 中国科学院华南植物研究所，广州 510650
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1997-07-10 发布日期:1997-07-10

Relationship Between Rice Leaf Senescence and Membrane Lipid Peroxidation During Grain Growth

Duan Jun; Liang Chengye; Zhang Mingyong; Huang Yuwen; Liu Hongxian   

  • Received:1900-01-01 Revised:1900-01-01 Online:1997-07-10 Published:1997-07-10

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被引次数

39

摘要： 以结实期间的稻为材料，在抽穗开花期通过剪去穗部部分枝梗和全穗等处理后，测定作为叶片衰老指标的叶绿素和蛋白质含量及作为膜脂过氧化指标的丙二醛含量的变化，探讨了水稻结实期间叶片衰老与膜脂过氧化之间的关系。试验结果指出，在水稻抽穗开花期间，剪去穗部枝梗或整个穗子，叶片中的叶绿素和蛋白质含量下降速率在处理后的7 d内就开始明显减慢，而MDA含量增加速率只有在处理7 d后才开始减缓，SOD活性并不因剪穗处理而有所提高。表明因剪穗(或穗部枝梗)而导致的叶绿素和蛋白质含量的变化早于MDA含量的变化，与SOD活性的变化无直接关系。说明在结实期间，水稻叶片在衰老过程中表现出的叶绿素和蛋白质含量的下降，并不主要是由于SOD的活性降低而导致膜脂过氧化作用增强所造成的结果，SOD活性下降和MDA含量的增加可能与叶绿素及蛋白质含量减少一样，只是叶片衰老开始后的一种结果，而非引起叶片衰老的原因。

关键词: 叶片, 衰老, 丙二醛, 超氧物歧化酶

Abstract: After 1/3 of spike and whole spike being removed at flowering stage, some physiological and biochemical indices of rice leaf senescence and membrane lipid peroxidation were determined. The results indicated that: Removing both 1/3 of spike and whole spike significantly delayed rice leaf senescence as indicated by higher chlorophyll and protein levels just after manipulative treatments, however, the membrane lipid peroxidation level decreased as indicated by lower malondialdehyde (MDA) content only after 7 days from manipulative treatments,the activity of superoxide dismutase (SOD) changed very little after treatments. The above results indicated that the membrane lipid peroxidation was not the primary cause of leaf senescence in rice at the grain development stage.

Key words: leaf, malondialdehyde, senescence, superoxide dismutase