中国水稻科学

中国水稻科学

• 研究简报 • 上一篇    下一篇

铜在水稻愈伤组织培养中的作用的进一步研究

杨跃生；郑贵朝；简玉瑜   

  1. 华南农业大学 生物技术学院， 广东 广州 510642
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:1999-10-10 发布日期:1999-10-10

A Further Study on the Effects of Copper in Rice Callus Culture (in English)

Yang Yuesheng; Zheng Guichao; Jian Yuyu   

  • Received:1900-01-01 Revised:1900-01-01 Online:1999-10-10 Published:1999-10-10

PDF

被引次数

67

摘要： 以籼稻栽培品种秋桂矮11的愈伤组织为材料，对铜元素的作用作了进一步的研究。在含有2,4-D和脯氨酸的培养基中添加铜元素对愈伤组织的增殖量影响极小，但转移到再分化培养基后这些愈伤组织能再生出显著更多的植株。另一方面，在含有BA、NAA和脯氨酸的培养基中添加铜元素则会由于添加量的不同而产生显著的促进或抑制愈伤组织增殖的作用。然而,不论是增殖受过铜的促进的或者是抑制的愈伤组织，均能在再分化培养基上再生出数量更多和质量更好的植株。

关键词: 组织培养, 增殖, 植株再生, 铜元素

Abstract: Calli of an indica rice cultivar Qiuguiai 11 were used as material for a further study on the effects of copper in calli cultures. Supplement of copper to a medium containing 2,4-D and proline had little effect on the proliferation rate of the calli, however, upon transfer to the plant regeneration medium, these calli would regenerate significantly more number of plants. On the other hand, supplement of copper to a medium containing proline, BA and NAA would significantly stimulate or suppressthe proliferation of the calli depending on the amount of copper supplemented. Nevertheless, all these calli, no matter the proliferation of which had been stimulated or suppressed by the copper, were able to regenerate more number of plants with higher quality on plant regeneration medium.

Key words: callus culture, proliferation, plant regeneration, copper, rice