Chinese Journal OF Rice Science >
Phenotypic Analysis and Gene Mapping of a Floury Endosperm Mutant we1 in Rice
Received date: 2024-05-15
Revised date: 2024-08-20
Online published: 2025-07-21
【Objective】 The study of floury endosperm mutants is helpful for elucidating the molecular mechanisms underlying rice grain quality formation. 【Method】 A stably inherited floury endosperm mutant we1 (white endosperm 1) was isolated from a 60Co-irradiated mutant pool of japonica rice cultivar Nipponbare. Phenotypic characterization and physicochemical analyses of we1 were conducted. An F2 population derived from a cross between we1 and indica variety 9311 was used for fine mapping. 【Result】 Compared with the wild type, we1 exhibited white, shrunken endosperm with irregularly shaped and loosely packed compound starch granules. The mutant showed significant reductions in 1000-grain weight, plant height, total starch content, and amylose content, along with a marked increase in lipid content. Genetic analysis indicated that the mutant phenotype was controlled by a single recessive nuclear gene. For map-based cloning, we1 was crossed with 9311 and recessive F2 individuals were selected. The WE1 locus was initially mapped to chromosome 6 and fine-mapped to a 181-kb physical interval. Twenty-three open reading frames (ORFs) were predicted in this region. qRT-PCR analysis revealed altered expression levels of starch synthesis-related genes in the we1 mutant. 【Conclusion】 WE1 likely encodes a novel gene involved in starch synthesis, providing a foundation for further elucidating its molecular regulatory mechanisms in rice.
Key words： rice; floury endosperm; starch; physicochemical property; fine mapping
ZHU Jianping, LI Xia, LI Wenqi, XU Yang, WANG Fangquan, TAO Yajun, JIANG Yanjie, CHEN Zhihui, FAN Fangjun, YANG Jie . Phenotypic Analysis and Gene Mapping of a Floury Endosperm Mutant we1 in Rice[J]. Chinese Journal OF Rice Science, 2025 , 39(4) : 543 -551 . DOI: 10.16819/j.1001-7216.2025.240508
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