Chinese Journal OF Rice Science >
Overexpression of RGG2, a Heterotrimeric G Protein γ Subunit-Encoding Gene, Improves Drought Tolerance in Rice
Received date: 2023-09-14
Revised date: 2023-12-08
Online published: 2024-05-13
【Objective】To investigate the role of RGG2, a heterotrimeric G protein γ subunit, in improving drought tolerance in rice.【Methods】The interaction between RGG2 and RGB1 was characterized using the yeast two-hybrid system and split luciferase complementation assays. The expression level of RGG2 and seed germination rates of transgenic lines were examined under exogenous ABA treatment conditions to determine whether RGG2 was involved in ABA response. The role of RGG2 in rice drought tolerance was analyzed by comparing the water loss rates of detached leaves and plant survival rates between wild-type and overexpression lines of RGG2 after drought treatment.【Results】RGG2 physically interacts with RGB1. The expression level of RGG2 was significantly induced by ABA, PEG-6000, and drought treatments. Overexpression lines of RGG2 in the backgrounds of Nipponbare and Wuyunjing 7 exhibited significantly lower seed germination and root length than the wild-type under ABA treatment condition, suggesting that RGG2 positively regulates ABA response. Compared with the wild-type, overexpression lines of RGG2 showed lower water loss of detached leaves and a higher survival ratio under drought treatment. After drought treatment, the expression levels of several ABA- and drought stress-related genes in the overexpression lines of RGG2 were higher than that in the wild-type.【Conclusion】RGG2 positively regulates ABA and drought stress responses, and overexpression of RGG2 improves drought tolerance in rice.
Key words： rice; heterotrimeric G protein; RGG2; ABA responses; drought stress
MIAO Jun, RAN Jinhui, XU Mengbin, BO Liubing, WANG Ping, LIANG Guohua, ZHOU Yong . Overexpression of RGG2, a Heterotrimeric G Protein γ Subunit-Encoding Gene, Improves Drought Tolerance in Rice[J]. Chinese Journal OF Rice Science, 2024 , 38(3) : 246 -255 . DOI: 10.16819/j.1001-7216.2024.230907
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