Chinese Journal OF Rice Science >
Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice
Received date: 2022-06-08
Revised date: 2022-08-31
Online published: 2023-03-10
【Objective】 The objective of this study is to elucidate the molecular and physiological mechanisms of the interaction between calcium ion (Ca2+) and hydrogen sulfide (H2S) alleviating aluminum (Al) toxicity in rice.【Method】 The rice cultivar kasalath was selected as test material, and treated with 0 μmol/L and 30 μmol/L AlCl3, 0.1 mmol/L and 0.5 mmol/L CaCl2, 0.2 μmol/L NaHS and 100 μmol/L H2S scavenger hypotaurine (HP). The seeds were incubated at 30℃ in the dark for 24 h, then the root elongation, total Al content, Al content in cell sap, Al content in apoplast, Al content in cell wall, pectin content, pectin methylesterase activity and the relative expression of OsSTAR2, OsNRAT1 and OsFRDL4 were measured to explore the molecular and physiological mechanism of the interaction between Ca2+ and H2S alleviating Al toxicity in rice. 【Result】 Under Al stress, compared with 0.1 mmol/L CaCl2 treatment, 0.5 mmol/L CaCl2 treatment significantly increased root elongation, H2S content, total calcium (Ca) content and cytoplasm Ca content, and significantly decreased total Al content in root, cell sap Al content, apoplastic Al content and cell wall Al content in rice. The pretreatment of NaHS significantly increased the elongation of rice roots at the two calcium concentrations, accompanied with the significant increase of root tip Al content, root total Al content, cell sap Al content, apoplastic Al content and cell wall Al content. The relative expression levels of OsSTAR2 and OsFRDL4 were significantly increased and the relative expression of OsNRAT1 was significantly decreased at the two Ca2+ concentrations after pretreated with NaHS. However, the application of HP produced an opposite result. 【Conclusion】 Under Al stress, Ca2+ reduced the accumulation of Al in rice root cell wall and the absorption of Al by rice roots through increasing the production of H2S in rice root, and finally alleviated the inhibition of Al on rice root elongation.
Key words： rice; aluminum stress; calcium ion; hydrogen sulfide
WEI Qianqian, XU Qingshan, PAN Lin, KONG Yali, ZHU Lianfeng, CAO Xiaochuang, TIAN Wenhao, LIU Jia, JIN Qianyu, XIANG Xingjia, ZHANG Junhua, ZHU Chunquan . Mechanism of Interaction Between Calcium Ion and Hydrogen Sulfide Alleviating the Inhibitory Effect of Aluminum on Root Elongation in Rice[J]. Chinese Journal OF Rice Science, 2023 , 37(2) : 142 -152 . DOI: 10.16819/j.1001-7216.2023.220603
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