Chinese Journal OF Rice Science >
Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9TQ and qSB-11HJX
Received date: 2022-04-24
Revised date: 2022-09-03
Online published: 2023-03-10
【Objective】 Blast and sheath blight are two major diseases in rice, causing huge yield and quality losses. Breeding resistant varieties is the most economical and effective approach to control the two diseases. 【Method】 Using molecular marker assisted selection, we introduced the broad-spectrum blast resistant gene Pigm and quantitative resistance genes qSB-9TQ, qSB-11HJX to sheath blight into Nanjing 9108, a japonica rice cultivar with good grain quality. Plant lines with different gene/gene combinations were obtained and the resistance phenotypes were identified as well as the main agronomic and quality traits. 【Result】 The inoculation results showed that Pigm introduction could increase rice resistance to seedling blast and panicle blast significantly. Both qSB-9TQ and qSB-11HJX could improve rice sheath blight resistance significantly. Moreover, pyramiding of the two quantitative resistance genes had an additive effect on sheath blight resistance. As for the main agronomic and quality traits, the lines with Pigm showed increased panicle length, number of grains per panicle and the lines with qSB-11HJX showed significantly increased grain weight. Incorporating these genes exerted no significant effect on other agronomic and quality traits. 【Conclusion】 Pyramiding the three genes could improve blast and sheath blight resistance without apparently negative effect on agronomic and quality traits, and the pyramided rice line can be used as a new germplasm for breeding disease-resistant japonica rice varieties.
Key words： blast; sheath blight; gene pyramiding; japonica rice; resistance improvement
WANG Yu, SUN Quanyi, DU Haibo, XU Zhiwen, WU Keting, YIN Li, FENG Zhiming, HU Keming, CHEN Zongxiang, ZUO Shimin . Improvement of the Resistance of Nanjing 9108 to Blast and Sheath Blight by Pyramiding Resistance Gene Pigm and Quantitative Trait Genes qSB-9TQ and qSB-11HJX[J]. Chinese Journal OF Rice Science, 2023 , 37(2) : 125 -132 . DOI: 10.16819/j.1001-7216.2023.220413
| [1] | Skamnioti P, Gurr S J. Against the grain: Safeguarding rice from rice blast disease[J]. Trends in Biotechnology, 2009, 27(3): 141-150. |
| [2] | 何峰, 张浩, 刘金灵, 王志龙, 王国梁. 水稻抗稻瘟病天然免疫机制及抗病育种新策略[J]. 遗传, 2014, 36(8): 756-765. |
| [2] | Heng F, Zhang H, Liu J L, Wang Z L, Wang G L. Recent advances in understanding the innate immune mechanisms and developing new disease resistance breeding strategies against the rice blast fungus Magnaporthe oryzae in rice[J]. Heraditas(Beijing), 2014, 36(8): 756-765. (in Chinese with English abstract) |
| [3] | Zheng A P, Lin R M, Zhang D H, Qin P G, Xu L Z, Ai P, Ding L, Wang Y R, Chen Y, Liu Y, Sun Z G, Feng H T, Liang X X, Fu R T, Tang C Q, Li Q, Zhang J, Xie Z L, Wang L X, Liu H N, Li P. The evolution and pathogenic mechanisms of the rice sheath blight pathogen[J]. Nature Communications, 2013, 4(1424): 1-10. |
| [4] | Lee N F, Rush M C. Rice sheath blight: A major rice disease[J]. Plant Disease, 1983, 6(7): 829-832. |
| [5] | Wang B H, Daniel J, Ebbole W Z, Wang Z H. The arms race between Magnaporthe oryzae and rice: Diversity and interaction of Avr and R genes[J]. Journal of Integrative Agriculture, 2017, 16(12): 2746-2760. |
| [6] | Chen S, Wang L, Que Z Q, Pan R Q. Genetic and physical mapping of pi37(t), a new gene conferring resistance to rice blast[J]. Theoretical and Applied Genetics, 2005, 111(8): 1563-1570. |
| [7] | Wang Z X, Yano M, Yamanouchi U, Iwamoto M, Monna L, Hayasaka H, Katayose Y, Sasaki T. The Pib gene for rice blast resistance belongs to the nucleotide binding and leucine-rich repeat class of plant disease resistance genes[J]. Plant Journal, 1999, 19(1): 55-64. |
| [8] | Zhou B, Qu S, Liu G, Dolan M, Sakai H, Lu G D, Bellizzi M, Wang G L. The eight amino-acid differences within three leucine-rich repeats between Pi2 and Piz-t resistance proteins determine the resistance specificity to Magnaporthe grisea[J]. Molecular Plant-Microbe Interactions, 2006, 19(11): 1216-1228. |
| [9] | Ashikawa I, Hayashi N, Yamane H, Kanamori H, Wu J Z, Matsumoto T, Ono K, Yano M. Two adjacent nucleotide-binding site-leucine-rich repeat class genes are required to confer Pikm-specific rice blast resistance[J]. Genetics, 2008, 180(4): 2267-2276. |
| [10] | Deng Y, Zhai K, Xie Z, Deng Y W, Zhai K R, Xie Z, Yang D Y, Zhu X D, Liu J Z, Wang X, Qin P, Yang Y Z, Zhang G M, Li Q, Zhang J F, Wu S Q, Milazzo J, Mao B Z, He Z H. Epigenetic regulation of antagonistic receptors confers rice blast resistance with yield balance[J]. Science, 2017, 355(6328): 962-965. |
| [11] | Bryan G T, Wu K S, Farrall L, Jia Y L, Hershey H, McAdams S, Faulk K, Donaldson G, Tarchini R, Valent B. A single amino acid difference distinguishes resistant and susceptible alleles of the rice blast resistance gene Pi-ta[J]. Plant Cell, 2000, 12(11): 2033-2045. |
| [12] | Rama Devi S J S, Singh K, Umakanth B, Vishalakshi B, Renu P, Sudhakar K V, Prasad M S, Viraktamath B C, Babu V R, Madhav M S. Development and identification of novel rice blast resistant sources and their characterization using molecular markers[J]. Rice Science, 2015, 22(6): 300-308. |
| [13] | 田红刚, 陈红旗, 胡江, 雷财林, 朱旭东, 钱前. 抗稻瘟病基因Pigm导入对寒地粳稻抗病性和产量性状的影响[J]. 沈阳农业大学学报, 2016, 47(5): 520-526. |
| [13] | Tian H G, Chen H Q, Hu J, Lei C L, Zhu X D, Qian Q. Effect of introgressed Pigm gene on rice blast resistance and yield traits of japonica rice in cold area[J]. Journal of Shenyang Agricultural University, 2016, 47(5): 520-526. (in Chinese with English abstract) |
| [14] | 张礼霞, 王林友, 范宏环, 王建军. 利用Pigm基因改良粳稻保持系的稻瘟病抗性研究[J]. 核农学报, 2017, 31(3): 424-431. |
| [14] | Zhang L X, Wang L Y, Fan H H, Wang J J. Improvement of rice blast resistance of japonica rice maintainer lines using gene Pigm[J]. Journal of Nuclear Agricultural Sciences, 2017, 31(3): 424-431. (in Chinese with English abstract) |
| [15] | 杨平, 邹国兴, 陈春莲, 黄永萍, 兰波, 熊运华, 尹建华. 利用分子标记辅助选择改良春恢350稻瘟病抗性[J]. 分子植物育种, 2015, 13(4): 741-747. |
| [15] | Yang P, Zou G X, Chen C L, Huang Y P, Lan B, Xiong Y H, Yin J H. Improvement of rice blast resistance of Chunhui 350 by using molecular-marker assisted selection[J]. Molecular Plant Breeding, 2015, 13(4): 741-747. (in Chinese with English abstract) |
| [16] | 陈涛, 孙旭超, 张善磊, 梁文化, 周丽慧, 赵庆勇, 姚姝, 赵凌, 赵春芳, 朱镇, 张亚东, 王才林. 稻瘟病广谱抗性基因Pigm特异性分子标记的开发和应用[J]. 中国水稻科学, 2020, 34(1): 28-36. |
| [16] | Chen T, Sun X C, Zhang S L, Liang W H, Zhou L H, Zhao Q Y, Yao S, Zhao L, Zhao C F, Zhu Z, Zhang Y D, Wang C L. Development and verification of specific molecular markers for Pigm gene associated with broad-spectrum resistance to rice blast[J]. Chinese Journal of Rice Science, 2020, 34(1): 28-36. (in Chinese with English abstract) |
| [17] | Zou J H, Pan X B, Chen Z X, Xu J Y, Lu J F, Zhai W X, Zhu L H. Mapping quantitative trait loci controlling sheath blight resistance in two rice cultivars[J]. Theoretical and Applied Genetics, 2000, 101(4): 569-573. |
| [18] | Pinson S R M, Capdevielle F M, Oard J H. Blight resistance in rice using recombinant inbred lines[J]. Crop Science, 2005, 45(2): 503-510. |
| [19] | Li Z, Pinson S R M, Marchetti M A, Stansel J W, Park W D. Characterization of quantitative trait loci (QTLs) in cultivated rice contributing to field resistance to sheath blight (Rhizoctonia solani)[J]. Theoretical and Applied Genetics, 1995, 91(2): 382-388. |
| [20] | 潘学彪, Rush M C. 美国的水稻纹枯病抗病遗传育种研究[J]. 江苏农学院学报, 1997, 18(1): 57-63. |
| [20] | Pan X B, Rush M C. Studies in the U.S. on genetics and breeding of resistance to rice sheath blight[J]. Journal of Jiangsu Agricultural College, 1997, 18(1): 57-63. (in Chinese with English abstract) |
| [21] | 左示敏, 张亚芳, 陈宗祥, 陈夕军, 潘学彪. 水稻抗纹枯病遗传育种研究进展[J]. 中国科学:生命科学, 2013, 40(11): 1014-1023. |
| [21] | Zuo S M, Zhang Y F, Chen Z X, Chen X J, Pan X B. Current progress on genetics and breeding in resistance to rice sheath blight[J]. Science China: Life Sciences, 2010, 40(11): 1014-1023. (in Chinese with English abstract) |
| [22] | 陈宗祥, 邹军煌, 徐敬友, 童蕴慧, 汤述翥, 王子斌, 蒋日民, 凌兵, 唐进, 潘学彪. 对水稻纹枯病抗源的初步研究[J]. 中国水稻科学, 2000, 14(1): 15-18. |
| [22] | Chen Z X, Zou J H, Xu J Y, Tong Y H, Tang S Z, Wang Z B, Jiang Y M, Ling B, Tang J, Pan X B. A preliminary study on resources of resistance to rice sheath blight[J]. Chinese Journal of Rice Science, 2000, 14(1): 15-18. (in Chinese with English abstract) |
| [23] | 殷跃军, 左示敏, 王辉, 张亚芳, 陈宗祥, 马玉银, 顾世梁, 潘学彪. 抗水稻纹枯病qSB-9Tq基因效应及作用方式分析[J]. 作物学报, 2009, 35(2): 279-285. |
| [23] | Yin Y J, Zuo S M, Wang H, Zhang Y F, Chen Z X, Ma Y Y, Gu S L, Pan X B. Effect and action analysis of qSB-9Tq conferring resistance to rice sheath blight[J]. Acta Agronomica Sinica, 2009, 35(2): 279-285. (in Chinese with English abstract) |
| [24] | Zhu Y J, Zuo S M, Chen Z X, Chen X G, Li G, Zhang Y F. Identification of two major rice sheath blight resistance QTLs, qSB1-1HJX74 and qSB11HJX74, in field trials using chromosome segment substitution lines[J]. Plant Disease, 2014, 98(8): 1112-1121. |
| [25] | 王才林, 张亚东, 朱镇, 姚姝, 赵庆勇, 陈涛, 周丽慧, 赵凌. 优良食味粳稻新品种南粳9108的选育与利用[J]. 江苏农业科学, 2013, 41(9): 86-88. |
| [25] | Wang C L, Zhang Y D, Zhu Z, Yao S, Zhao Q Y, Chen T, Zhou L H, Zhao L. Breeding and utilization of a new japonica rice variety Nanjing 9108 with good taste[J]. Jiangsu Agricultural Sciences, 2013, 41(9): 86-88. (in Chinese with English abstract) |
| [26] | 张亚东, 姚姝, 陈涛, 王军, 朱镇, 赵庆勇, 周丽慧, 赵凌, 赵春芳, 路凯, 梁文化, 王才林. 聚合Wx-mp、fgr和Pi-ta、Pi-b基因选育优质粳稻新品种[J]. 分子植物育种, 2021, https://kns.cnki.net/kcms/detail/46.1068.S.20210709.1328.025.html |
| [26] | Zhang Y D, Yao S, Chen T, Wang J, Zhu Z, Zhao Q Y, Zhou L H, Zhao L, Zhao C F, Lu K, Liang W H, Wang C L. Pyramiding Wx-mp, fgr and Pi-ta, Pi-b genes by marker-assisted selection in new japonica rice varieties with good quality[J]. Molecular Plant Breeding, 2021. https://kns.cnki.net/kcms/detail/46.1068.S.20210709.1328.025.html (in Chinese with English abstract) |
| [27] | 李明友, 王嘉楠, 王广达, 冯志明, 叶英豪, 姜伟, 左天, 张亚芳, 陈夕军, 潘学彪. 抗纹枯病数量性状基因qSB-11HJX及qSB-9TQ改良粳稻品种的抗性研究[J]. 扬州大学学报: 农业与生命科学版, 2019, 40(6): 1-7. |
| [27] | Li M Y, Wang J N, Wang G D, Feng Z M, Ye Y H, Jiang W, Zuo T, Zhang Y F, Chen X J, Pan X B. Improvement of japonica rice resistance to sheath blight disease by incorporating quantitative resistance genes qSB-11HJX and qSB-9TQ[J]. Journal of Yangzhou University: Agricultural and Life Science Edition, 2019, 40(6): 1-7. (in Chinese with English abstract) |
| [28] | 贺闽, 尹俊杰, 冯志明, 朱孝波, 赵剑华, 左示敏, 陈学伟. 水稻稻瘟病和纹枯病抗性鉴定方法[J]. 植物学报, 2020, 55(5): 577-587. |
| [28] | He M, Yin J J, Feng Z M, Zhu X B, Zhao J H, Zuo S M, Chen X W. Identification methods of resistance to rice blast and sheath blight[J]. Chinese Bulletin of Botany, 2020, 55(5): 577-587. (in Chinese with English abstract) |
| [29] | 王小秋. 江苏粳稻抗稻瘟病基因应用与稻瘟菌多样性分析[D]. 扬州: 扬州大学, 2020. |
| [29] | Wang X Q. Research on blast resistance genes utilized in japonica rice and analysis of blast isolates diversity in Jiangsu Province[D]. Yangzhou: Yangzhou University, 2020. (in Chinese with English abstract) |
| [30] | 左示敏, 张亚芳, 殷跃军, 陈宗祥, 潘学彪. 田间水稻纹枯病抗性鉴定体系的确立与完善[J]. 扬州大学学报:农业与生命科学版, 2006, 27(4): 57-61. |
| [30] | Zuo S M, Zhang Y F, Yin Y J, Chen Z X, Pan X B. Establishment and improvement of inoculation technique and rating system in researching rice sheath blight resistance in field[J]. Journal of Yangzhou University: Agricultural and Life Science Edition, 2006, 27(4): 57-61. (in Chinese with English abstract) |
| [31] | 朱大伟. 三种关键栽培措施对软米粳稻产量与品质的影响[D]. 扬州: 扬州大学, 2018. |
| [31] | Zhu D W. Effect of three key cultivation measures on yield and quality of japonica soft rice[D]. Yangzhou: Yangzhou University, 2020. (in Chinese with English abstract) |
| [32] | Liu Y, Chen L, Fu D, Lou Q J, Mei H W, Xiong L, Li M S, Xu X Y, Mei X H, Luo L J. Dissection of additive, epistatic effect and QTL × environment interaction of quantitative trait loci for sheath blight resistance in rice[J]. Hereditas, 2014, 151(2-3): 28-37. |
| [33] | Eizenga G C, Prasad B, Jackson A K, Jia M H. Identification of rice sheath blight and blast quantitative trait loci in two different O. sativa/O. nivara advanced backcross populations[J]. Molecular Breeding, 2013, 31(4): 889-907. |
| [34] | 陈宗祥, 冯志明, 王龙平, 冯凡, 张亚芳, 马玉银, 潘学彪, 左示敏. 水稻分蘖角基因TAC1的育种应用价值分析[J]. 中国水稻科学, 2017, 31(6): 590-598. |
| [34] | Chen Z X, Feng Z M, Wang L P, Feng F, Zhang Y F, Ma Y Y, Pan X B, Zuo S M. Breeding potential of rice TAC1 gene for tiller angle[J]. Chinese Journal of Rice Science, 2017, 31(6): 590-598. (in Chinese with English abstract) |
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