Chinese Journal OF Rice Science >
Transcriptome Analysis of Young Rice Panicles in Early Response to Exposure to Mycotoxin of Ustilaginoidea virens
Received date: 2021-09-06
Revised date: 2021-11-10
Online published: 2022-09-09
【Objective】 Rice false smut caused by Ustilaginoidea virens not only leads to rice yield loss, but also produces mycotoxins which is toxic to animals and plants. In order to elucidate the molecular mechanism behind the response to exposure to mycotoxin the young panicles of rice were mycotoxin-treated with U. virens mycotoxin, which may provide a new idea for exploring rice false smut resistance genes and molecular breeding for disease resistance. 【Method】Transcriptome sequencing was performed with mycotoxin-treated young panicles as materials. The genome of 9311 was used as the reference genome to align the sequences. The expression level of genes was calculated by the term of Transcripts Per Million (TPM). According to the database, the differentially expressed genes were screened by parameters (|LOG2 fold change|≥1 and q-value≤0.05). Combined with differential expression analysis and function annotation, the key genes in response to mycotoxin were identified in rice panicles. The differentially expressed genes were verified by qRT-PCR. 【Result】2526 differentially expressed genes (DEG) were identified in young panicles of rice after 12 h treated with mycotoxin. Through GO enrichment, KEGG metabolic pathway and KOG function analysis, the DEGs were divided into 64 terms under GO function, 32 metabolic pathways and 23 categories under KOG function, including starch and sucrose metabolism, phenylpropyl biosynthesis, carbon metabolism, glycolysis/gluconeogenesis, amino sugar and nucleoside sugar metabolism and other biological processes. There were 66 plant transcription factors in DEGs, which belonged to 7 family of plant transcription factors, including WRKY and Myb. The expression levels of OsCPS2, OSKSL4 and cytochrome P450 were up-regulated, while the expression levels of amylase, beta-fructofuranosidase and UGPase were down-regulated when we analyzed genes involved in diterpenoid biosynthesis and starch and sucrose metabolism pathways. Therefore, it is speculated that these genes play an important role in rice response to mycotoxin of U. virens stress. 【Conclusion】As an abiotic stress factor, mycotoxin of U. virens had a toxic effect on young panicles of rice. It affects the synthesis of nutrients in seeds by interfering with metabolism of starch and sucrose, and thus reduces the resistance of rice to pathogen infection.
Key words： rice; Ustilaginoidea virens; mycotoxin; transcriptome analysis
FU Rongtao, WANG Jian, CHEN Cheng, ZHAO Liyu, CHEN Xuejuan, LU Daihua . Transcriptome Analysis of Young Rice Panicles in Early Response to Exposure to Mycotoxin of Ustilaginoidea virens[J]. Chinese Journal OF Rice Science, 2022 , 36(5) : 447 -458 . DOI: 10.16819/j.1001-7216.2022.210714
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