Chinese Journal OF Rice Science >
Cytological Observation of a Female and Male Sterile Osfma2 Mutant in Rice and Its Map-based Cloning
#These authors contributed equally to this work;
Received date: 2021-03-23
Revised date: 2021-04-22
Online published: 2022-01-10
【Objective】 The work aims to locate and clone the rice female and male sterile gene OsFMA2 by constructing a segregated population and explore its function in the regulation of rice fertility. 【Method】 Stable sterile mutants were obtained by EMS mutagenesis of japonica rice Ningjing 4, and the phenotypic and cytological observation of the mutant was conducted. Using map-based cloning and Mut-map methods, the female and male sterile gene OsFMA2 was fine-mapped and cloned. The expression pattern of OsFMA2 in various tissues was analyzed by the real-time quantitative PCR technique. Subcellular localization of OsFMA2 protein was performed with the rice protoplast expression system. 【Result】 The cytological observation revealed that the Osfma2 mutant was male- and female-sterile, and the extreme individuals were used to locate the gene in a 448-kb interval on the long arm of chromosome 6. The gene OsFMA2 is predicted to encode a replication protein that is highly conserved in monocots. The gene OsFMA2 was tissue-specific and was highly expressed in young panicles. Subcellular localization analysis showed that the OsFMA2 protein was localized on the nucleus. 【Conclusion】The gene OsFMA2 is highly expressed in young panicles, and it may participate in the homologous recombination of the first meiotic division of male gamete. At the same time, the expression of OsFMA2 also has an adverse effect on the development of female gametes.
ZHANG Taohui, WANG Haiyu, WAN Hua, ZHANG Liping, XIE Zhenwei, CHEN Keyi, HE Xiaodong, ZHAO Zhigang, WAN Jianmin . Cytological Observation of a Female and Male Sterile Osfma2 Mutant in Rice and Its Map-based Cloning[J]. Chinese Journal OF Rice Science, 2022 , 36(1) : 13 -26 . DOI: 10.16819/j.1001-7216.2022.210312
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